In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study

Porcupine bezoar (PB) was reported to possess medicinal properties in old medical manuscript. However, its potential as anticancer agent on human lung cancer cells (A549) is not yet studied. In present study, porcupine bezoar was tested to observe its ability in inhibiting cell growth of cancer cell...

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Main Authors:	Firus Khan, Al'aina Yuhainis, Mohd Nasir, Mohd Hamzah, Jalal, Tara Kamal, Razali, Shakirah, Hassan, Habibah, Abdullah Asuhaimi, Faizah, Abdul Wahab, Ridhwan
Format:	Article
Language:	English
Published:	Juniper Publishers 2017
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/1/a549.pdf

id	iium-60815
recordtype	eprints
spelling	iium-608152018-01-05T03:16:03Z http://irep.iium.edu.my/60815/ In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study Firus Khan, Al'aina Yuhainis Mohd Nasir, Mohd Hamzah Jalal, Tara Kamal Razali, Shakirah Hassan, Habibah Abdullah Asuhaimi, Faizah Abdul Wahab, Ridhwan RM Therapeutics. Pharmacology Porcupine bezoar (PB) was reported to possess medicinal properties in old medical manuscript. However, its potential as anticancer agent on human lung cancer cells (A549) is not yet studied. In present study, porcupine bezoar was tested to observe its ability in inhibiting cell growth of cancer cell (A549) and its cytotoxicity on Normal Human Gingival Fibroblast Cell (HGF-1). A549 cells morphology was observed after treated with bezoars for 72 hours. The ability of bezoars to induce DNA damage and apoptosis was analyzed by staining cells with Hoechst 33428(nucleus) and Rhodamine Phalloidin (f-actin). The A549 IC50 is 13.6±1.58μg/ml A549 growths was inhibited in dose-dependent pattern, but no inhibition found on normal HGF-1 cells. Treated A549 morphology shows sign of apoptosis such as DNA fragmentation, cytoplasm shrunk and vacuolation. The finding in this study suggests PB extracts able to inhibit cell growth, induce DNA damage and apoptosis, further analysis need to be done to verify the mechanism. Juniper Publishers 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60815/1/a549.pdf Firus Khan, Al'aina Yuhainis and Mohd Nasir, Mohd Hamzah and Jalal, Tara Kamal and Razali, Shakirah and Hassan, Habibah and Abdullah Asuhaimi, Faizah and Abdul Wahab, Ridhwan (2017) In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study. Advances in Biotechnology and Microbiology, 5 (1). pp. 1-8. ISSN 2474-7637 http://juniperpublishers.com/aibm/pdf/AIBM.MS.ID.555651.pdf 10.19080/AIBM.2017.05.555651
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Firus Khan, Al'aina Yuhainis Mohd Nasir, Mohd Hamzah Jalal, Tara Kamal Razali, Shakirah Hassan, Habibah Abdullah Asuhaimi, Faizah Abdul Wahab, Ridhwan In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
description	Porcupine bezoar (PB) was reported to possess medicinal properties in old medical manuscript. However, its potential as anticancer agent on human lung cancer cells (A549) is not yet studied. In present study, porcupine bezoar was tested to observe its ability in inhibiting cell growth of cancer cell (A549) and its cytotoxicity on Normal Human Gingival Fibroblast Cell (HGF-1). A549 cells morphology was observed after treated with bezoars for 72 hours. The ability of bezoars to induce DNA damage and apoptosis was analyzed by staining cells with Hoechst 33428(nucleus) and Rhodamine Phalloidin (f-actin). The A549 IC50 is 13.6±1.58μg/ml A549 growths was inhibited in dose-dependent pattern, but no inhibition found on normal HGF-1 cells. Treated A549 morphology shows sign of apoptosis such as DNA fragmentation, cytoplasm shrunk and vacuolation. The finding in this study suggests PB extracts able to inhibit cell growth, induce DNA damage and apoptosis, further analysis need to be done to verify the mechanism.
format	Article
author	Firus Khan, Al'aina Yuhainis Mohd Nasir, Mohd Hamzah Jalal, Tara Kamal Razali, Shakirah Hassan, Habibah Abdullah Asuhaimi, Faizah Abdul Wahab, Ridhwan
author_facet	Firus Khan, Al'aina Yuhainis Mohd Nasir, Mohd Hamzah Jalal, Tara Kamal Razali, Shakirah Hassan, Habibah Abdullah Asuhaimi, Faizah Abdul Wahab, Ridhwan
author_sort	Firus Khan, Al'aina Yuhainis
title	In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
title_short	In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
title_full	In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
title_fullStr	In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
title_full_unstemmed	In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study
title_sort	in vitro evaluation of porcupine bezoar extracts as anticancer agent on a549 -a preliminary study
publisher	Juniper Publishers
publishDate	2017
url	http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/ http://irep.iium.edu.my/60815/1/a549.pdf
first_indexed	2023-09-18T21:26:12Z
last_indexed	2023-09-18T21:26:12Z
_version_	1777412208548904960

In vitro evaluation of porcupine bezoar extracts as anticancer agent on A549 -A preliminary study

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