Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells

Porcupine bezoar (PB) previously reported to possess various medical properties, however there is no information on its potential anticancer effect on human cancer cells. In this study we investigate potential apoptotic effects induced by PB extracts in A549 cells and elucidate the underlying mec...

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Main Authors:	Firus Khan, Al'aina Yuhainis, Abdullah Asuhaimi, Faizah, Abdull Rasad, Mohammad Syaiful Bahari, Mohamad, Mardhiah, Hashim, Ridzwan, Mamat, Suhana, Johan, Muhammad Farid, Abdul Wahab, Ridhwan
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/60467/ http://irep.iium.edu.my/60467/1/pan%20asian.pdf

id	iium-60467
recordtype	eprints
spelling	iium-604672018-01-22T07:12:56Z http://irep.iium.edu.my/60467/ Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells Firus Khan, Al'aina Yuhainis Abdullah Asuhaimi, Faizah Abdull Rasad, Mohammad Syaiful Bahari Mohamad, Mardhiah Hashim, Ridzwan Mamat, Suhana Johan, Muhammad Farid Abdul Wahab, Ridhwan RM Therapeutics. Pharmacology Porcupine bezoar (PB) previously reported to possess various medical properties, however there is no information on its potential anticancer effect on human cancer cells. In this study we investigate potential apoptotic effects induced by PB extracts in A549 cells and elucidate the underlying mechanism. Our study revealed that PB reduced the viability of A549 cells in dose dependent manner. PB treatment shown induction morphological changes of apoptosis feature in our phase contrast, hoechst 33342 and rhodamine phalloidin counter staining analysis. Flow cytometric analysis revealed that PB led to cell cycle arrest at G0/G1 through promoting p21 and inhibiting cyclin D/CDK1 complex gene expressions. Apoptotic gene expressions results indicated that PB mediated apoptosis of A549 cells by mitochondria pathway through receptor. Evidenced up-regulation of caspase 8 and BCL-2 interacting domain (BID) supported which resulted in Cytochrome C released. Additionally, upregulation of Bax result in down regulation of Bcl-2. The activities of caspase-8 and caspase-9 were enhanced by PB promoting caspase-3 activation, leading to DNA fragmentation. In conclusion, PB induced apoptosis occurs via mitochondria dependent pathway through receptor. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/60467/1/pan%20asian.pdf Firus Khan, Al'aina Yuhainis and Abdullah Asuhaimi, Faizah and Abdull Rasad, Mohammad Syaiful Bahari and Mohamad, Mardhiah and Hashim, Ridzwan and Mamat, Suhana and Johan, Muhammad Farid and Abdul Wahab, Ridhwan (2016) Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells. In: 3rd Pan-Asian Biomedical Science Conference 2016 (3rd PABSC), 7th–8th December 2016, Shah Alam, Selangor. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Firus Khan, Al'aina Yuhainis Abdullah Asuhaimi, Faizah Abdull Rasad, Mohammad Syaiful Bahari Mohamad, Mardhiah Hashim, Ridzwan Mamat, Suhana Johan, Muhammad Farid Abdul Wahab, Ridhwan Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
description	Porcupine bezoar (PB) previously reported to possess various medical properties, however there is no information on its potential anticancer effect on human cancer cells. In this study we investigate potential apoptotic effects induced by PB extracts in A549 cells and elucidate the underlying mechanism. Our study revealed that PB reduced the viability of A549 cells in dose dependent manner. PB treatment shown induction morphological changes of apoptosis feature in our phase contrast, hoechst 33342 and rhodamine phalloidin counter staining analysis. Flow cytometric analysis revealed that PB led to cell cycle arrest at G0/G1 through promoting p21 and inhibiting cyclin D/CDK1 complex gene expressions. Apoptotic gene expressions results indicated that PB mediated apoptosis of A549 cells by mitochondria pathway through receptor. Evidenced up-regulation of caspase 8 and BCL-2 interacting domain (BID) supported which resulted in Cytochrome C released. Additionally, upregulation of Bax result in down regulation of Bcl-2. The activities of caspase-8 and caspase-9 were enhanced by PB promoting caspase-3 activation, leading to DNA fragmentation. In conclusion, PB induced apoptosis occurs via mitochondria dependent pathway through receptor.
format	Conference or Workshop Item
author	Firus Khan, Al'aina Yuhainis Abdullah Asuhaimi, Faizah Abdull Rasad, Mohammad Syaiful Bahari Mohamad, Mardhiah Hashim, Ridzwan Mamat, Suhana Johan, Muhammad Farid Abdul Wahab, Ridhwan
author_facet	Firus Khan, Al'aina Yuhainis Abdullah Asuhaimi, Faizah Abdull Rasad, Mohammad Syaiful Bahari Mohamad, Mardhiah Hashim, Ridzwan Mamat, Suhana Johan, Muhammad Farid Abdul Wahab, Ridhwan
author_sort	Firus Khan, Al'aina Yuhainis
title	Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
title_short	Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
title_full	Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
title_fullStr	Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
title_full_unstemmed	Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells
title_sort	porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /cdk1 complex and apoptosis mitochondria mediated dependent pathway in a549 cells
publishDate	2016
url	http://irep.iium.edu.my/60467/ http://irep.iium.edu.my/60467/1/pan%20asian.pdf
first_indexed	2023-09-18T21:25:44Z
last_indexed	2023-09-18T21:25:44Z
_version_	1777412178644566016

Porcupine bezoar exhibit cell cycle arrest through inhibiting cyclin /CDK1 complex and apoptosis mitochondria mediated dependent pathway in A549 cells

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