Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges

Sponges are sources of various useful natural products. The natural compounds derived from sponges are mostly complex polyketide. The polyketide synthase genes (PKS) is responsible for the biosynthesis of complex polyketides. During this research, we detected ketosynthase (KS) gene domain from 10 sp...

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Main Authors:	Atikana, Akhirta, Mohamad, Mohd Azrul Naim, Sipkema, Detmer
Format:	Article
Language:	English
Published:	Indonesian Institute of Sciences (LIPI) 2013
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/60315/ http://irep.iium.edu.my/60315/ http://irep.iium.edu.my/60315/1/78-183-1-PB.pdf

id	iium-60315
recordtype	eprints
spelling	iium-603152017-12-22T03:57:33Z http://irep.iium.edu.my/60315/ Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges Atikana, Akhirta Mohamad, Mohd Azrul Naim Sipkema, Detmer QR Microbiology Sponges are sources of various useful natural products. The natural compounds derived from sponges are mostly complex polyketide. The polyketide synthase genes (PKS) is responsible for the biosynthesis of complex polyketides. During this research, we detected ketosynthase (KS) gene domain from 10 species of marine sponges, collected from Mediterranean Sea (Aplysina aerophoba, Petrocia ficiformis, Axinella damicornis, Axinella veronicas, Crambe crambe) and North Sea (Halichondria panicea, Haliclona xena, Haliclona oculata, Suberites massa, Ephydatia fluviatilis). We also detected KS gene domain from 12 bacterial sponge isolated from H. panacea. We used a PCR-based approach to detect the ketosynthase (KS) gene domain of Polyketosynthase (PKS) genes. Our result showed that the sponges and the bacterial sponges are mostly KS-positive (700 bp). The presence of the PKS gene indicates the ability of the sponges to produce bioactive compound and potential natural product. The presence of the KS domain in both of the sponges and the bacterial sponges might also indicate that the bacterial sponges involved in the biosintesis of secondary metabolite of the sponges Indonesian Institute of Sciences (LIPI) 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60315/1/78-183-1-PB.pdf Atikana, Akhirta and Mohamad, Mohd Azrul Naim and Sipkema, Detmer (2013) Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges. Annales Bogoriensis, 17 (2). pp. 27-33. ISSN 0517-8452 E-ISSN 2407-7518 http://jurnal.biotek.lipi.go.id/index.php/annales/issue/view/24
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Atikana, Akhirta Mohamad, Mohd Azrul Naim Sipkema, Detmer Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
description	Sponges are sources of various useful natural products. The natural compounds derived from sponges are mostly complex polyketide. The polyketide synthase genes (PKS) is responsible for the biosynthesis of complex polyketides. During this research, we detected ketosynthase (KS) gene domain from 10 species of marine sponges, collected from Mediterranean Sea (Aplysina aerophoba, Petrocia ficiformis, Axinella damicornis, Axinella veronicas, Crambe crambe) and North Sea (Halichondria panicea, Haliclona xena, Haliclona oculata, Suberites massa, Ephydatia fluviatilis). We also detected KS gene domain from 12 bacterial sponge isolated from H. panacea. We used a PCR-based approach to detect the ketosynthase (KS) gene domain of Polyketosynthase (PKS) genes. Our result showed that the sponges and the bacterial sponges are mostly KS-positive (700 bp). The presence of the PKS gene indicates the ability of the sponges to produce bioactive compound and potential natural product. The presence of the KS domain in both of the sponges and the bacterial sponges might also indicate that the bacterial sponges involved in the biosintesis of secondary metabolite of the sponges
format	Article
author	Atikana, Akhirta Mohamad, Mohd Azrul Naim Sipkema, Detmer
author_facet	Atikana, Akhirta Mohamad, Mohd Azrul Naim Sipkema, Detmer
author_sort	Atikana, Akhirta
title	Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
title_short	Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
title_full	Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
title_fullStr	Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
title_full_unstemmed	Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges
title_sort	detection of keto synthase (ks) gene domain in sponges and bacterial sponges
publisher	Indonesian Institute of Sciences (LIPI)
publishDate	2013
url	http://irep.iium.edu.my/60315/ http://irep.iium.edu.my/60315/ http://irep.iium.edu.my/60315/1/78-183-1-PB.pdf
first_indexed	2023-09-18T21:25:30Z
last_indexed	2023-09-18T21:25:30Z
_version_	1777412163622666240

Detection of Keto Synthase (KS) gene domain in sponges and bacterial sponges

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