Fungi found in Mediterranean and North Sea sponges: how specific are they?

Fungi and other eukaryotes represent one of the last frontiers of microbial diversity in the sponge holobiont. In this study we employed pyrosequencing of 18S ribosomal RNA gene amplicons containing the V7 and V8 hypervariable regions to explore the fungal diversity of seven sponge species from the...

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Main Authors:	Mohamad, Mohd Azrul Naim, Smidt, Hauke, Sipkema, Detmer
Format:	Article
Language:	English English
Published:	PeerJ Inc. 2017
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/1/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea.pdf http://irep.iium.edu.my/60310/2/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea_SCOPUS.pdf

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recordtype	eprints
spelling	iium-603102018-04-27T08:38:03Z http://irep.iium.edu.my/60310/ Fungi found in Mediterranean and North Sea sponges: how specific are they? Mohamad, Mohd Azrul Naim Smidt, Hauke Sipkema, Detmer QR Microbiology Fungi and other eukaryotes represent one of the last frontiers of microbial diversity in the sponge holobiont. In this study we employed pyrosequencing of 18S ribosomal RNA gene amplicons containing the V7 and V8 hypervariable regions to explore the fungal diversity of seven sponge species from the North Sea and the Mediterranean Sea. For most sponges, fungi were present at a low relative abundance averaging 0.75% of the 18S rRNA gene reads. In total, 44 fungal OTUs (operational taxonomic units) were detected in sponges, and 28 of these OTUs were also found in seawater. Twenty-two of the sponge-associated OTUs were identified as yeasts (mainly Malasseziales), representing 84% of the fungal reads. Several OTUs were related to fungal sequences previously retrieved from other sponges, but all OTUs were also related to fungi from other biological sources, such as seawater, sediments, lakes and anaerobic digesters. Therefore our data, supported by currently available data, point in the direction of mostly accidental presence of fungi in sponges and do not support the existence of a sponge-specific fungal community. PeerJ Inc. 2017-09-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60310/1/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea.pdf application/pdf en http://irep.iium.edu.my/60310/2/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea_SCOPUS.pdf Mohamad, Mohd Azrul Naim and Smidt, Hauke and Sipkema, Detmer (2017) Fungi found in Mediterranean and North Sea sponges: how specific are they? PeerJ, 2017 (9). pp. 1-19. ISSN 2167-8359 https://peerj.com/articles/3722.pdf 10.7717/peerj.3722
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohamad, Mohd Azrul Naim Smidt, Hauke Sipkema, Detmer Fungi found in Mediterranean and North Sea sponges: how specific are they?
description	Fungi and other eukaryotes represent one of the last frontiers of microbial diversity in the sponge holobiont. In this study we employed pyrosequencing of 18S ribosomal RNA gene amplicons containing the V7 and V8 hypervariable regions to explore the fungal diversity of seven sponge species from the North Sea and the Mediterranean Sea. For most sponges, fungi were present at a low relative abundance averaging 0.75% of the 18S rRNA gene reads. In total, 44 fungal OTUs (operational taxonomic units) were detected in sponges, and 28 of these OTUs were also found in seawater. Twenty-two of the sponge-associated OTUs were identified as yeasts (mainly Malasseziales), representing 84% of the fungal reads. Several OTUs were related to fungal sequences previously retrieved from other sponges, but all OTUs were also related to fungi from other biological sources, such as seawater, sediments, lakes and anaerobic digesters. Therefore our data, supported by currently available data, point in the direction of mostly accidental presence of fungi in sponges and do not support the existence of a sponge-specific fungal community.
format	Article
author	Mohamad, Mohd Azrul Naim Smidt, Hauke Sipkema, Detmer
author_facet	Mohamad, Mohd Azrul Naim Smidt, Hauke Sipkema, Detmer
author_sort	Mohamad, Mohd Azrul Naim
title	Fungi found in Mediterranean and North Sea sponges: how specific are they?
title_short	Fungi found in Mediterranean and North Sea sponges: how specific are they?
title_full	Fungi found in Mediterranean and North Sea sponges: how specific are they?
title_fullStr	Fungi found in Mediterranean and North Sea sponges: how specific are they?
title_full_unstemmed	Fungi found in Mediterranean and North Sea sponges: how specific are they?
title_sort	fungi found in mediterranean and north sea sponges: how specific are they?
publisher	PeerJ Inc.
publishDate	2017
url	http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/ http://irep.iium.edu.my/60310/1/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea.pdf http://irep.iium.edu.my/60310/2/60310_Fungi%20found%20in%20Mediterranean%20and%20North%20Sea_SCOPUS.pdf
first_indexed	2023-09-18T21:25:29Z
last_indexed	2023-09-18T21:25:29Z
_version_	1777412163329064960

Fungi found in Mediterranean and North Sea sponges: how specific are they?

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