Wound healing properties of biotransformed asiaticoside by aspergillus niger

Biotransformation is extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmacologically-active compounds. This study investigated the effects of asiaticoside and its biotransformed product on wound healing...

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Main Authors:	Omar, Muhammad Nor, Alfarra, Helmi Yousi, Ichwan, Solachuddin Jauhari Arief
Format:	Article
Language:	English English
Published:	Penerbit UMT 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/60306/ http://irep.iium.edu.my/60306/ http://irep.iium.edu.my/60306/1/PROF%20NO%20JSSM.pdf http://irep.iium.edu.my/60306/9/60306_Wound%20healing%20properties%20of%20biotransformed%20asiaticoside_SCOPUS.pdf

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recordtype	eprints
spelling	iium-603062018-03-08T03:29:18Z http://irep.iium.edu.my/60306/ Wound healing properties of biotransformed asiaticoside by aspergillus niger Omar, Muhammad Nor Alfarra, Helmi Yousi Ichwan, Solachuddin Jauhari Arief QD Chemistry Biotransformation is extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmacologically-active compounds. This study investigated the effects of asiaticoside and its biotransformed product on wound healing activities. Asiaticoside, the bioactive constituent of Centella asiatica has been reported to possess wound healing properties. Microbial transformation of asiaticoside using Aspergillus niger was carried out to produce an asiaticoside biotransformed product and the wound healing activities of asiaticoside and its biotransformed product were investigated. Their effects on transforming growth factor-beta 1 (TGFβ1) and tissue inhibitor of metalloproteinase 1 (TIMP1) gene expression were examined to understand the mode of action and on the cell proliferation and wound healing using human keratinocytes. Results of in vitro study showed that asiaticoside concentrations between 7.5 and 120 μg/mL gave higher value of cell proliferation than the negative control. On the other hand, the biotransformed product concentrations between 0.058 and 3.75 μg/mL exhibited high cell viability but the viability was lowest at 15.0 μg/mL, suggesting cytotoxic effects on the cells. In wound healing assays, there were significant differences on wound closure in comparison to the negative control (P<0.05). Both asiaticoside and the biotransformed product increased the expression of TGFβ1 and TIMP1 respectively, with the latter showing more enhanced expressions of both genes. The biotransformed product also showed faster migration and healing rate under microscopic observation. Penerbit UMT 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60306/1/PROF%20NO%20JSSM.pdf application/pdf en http://irep.iium.edu.my/60306/9/60306_Wound%20healing%20properties%20of%20biotransformed%20asiaticoside_SCOPUS.pdf Omar, Muhammad Nor and Alfarra, Helmi Yousi and Ichwan, Solachuddin Jauhari Arief (2017) Wound healing properties of biotransformed asiaticoside by aspergillus niger. Journal of Sustainability Science and Management, 12 (2). pp. 96-102. ISSN 1823-8556 http://jssm.umt.edu.my
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	QD Chemistry
spellingShingle	QD Chemistry Omar, Muhammad Nor Alfarra, Helmi Yousi Ichwan, Solachuddin Jauhari Arief Wound healing properties of biotransformed asiaticoside by aspergillus niger
description	Biotransformation is extensively used to create useful metabolites from various natural products and as an alternative to chemical synthesis for the preparation of pharmacologically-active compounds. This study investigated the effects of asiaticoside and its biotransformed product on wound healing activities. Asiaticoside, the bioactive constituent of Centella asiatica has been reported to possess wound healing properties. Microbial transformation of asiaticoside using Aspergillus niger was carried out to produce an asiaticoside biotransformed product and the wound healing activities of asiaticoside and its biotransformed product were investigated. Their effects on transforming growth factor-beta 1 (TGFβ1) and tissue inhibitor of metalloproteinase 1 (TIMP1) gene expression were examined to understand the mode of action and on the cell proliferation and wound healing using human keratinocytes. Results of in vitro study showed that asiaticoside concentrations between 7.5 and 120 μg/mL gave higher value of cell proliferation than the negative control. On the other hand, the biotransformed product concentrations between 0.058 and 3.75 μg/mL exhibited high cell viability but the viability was lowest at 15.0 μg/mL, suggesting cytotoxic effects on the cells. In wound healing assays, there were significant differences on wound closure in comparison to the negative control (P<0.05). Both asiaticoside and the biotransformed product increased the expression of TGFβ1 and TIMP1 respectively, with the latter showing more enhanced expressions of both genes. The biotransformed product also showed faster migration and healing rate under microscopic observation.
format	Article
author	Omar, Muhammad Nor Alfarra, Helmi Yousi Ichwan, Solachuddin Jauhari Arief
author_facet	Omar, Muhammad Nor Alfarra, Helmi Yousi Ichwan, Solachuddin Jauhari Arief
author_sort	Omar, Muhammad Nor
title	Wound healing properties of biotransformed asiaticoside by aspergillus niger
title_short	Wound healing properties of biotransformed asiaticoside by aspergillus niger
title_full	Wound healing properties of biotransformed asiaticoside by aspergillus niger
title_fullStr	Wound healing properties of biotransformed asiaticoside by aspergillus niger
title_full_unstemmed	Wound healing properties of biotransformed asiaticoside by aspergillus niger
title_sort	wound healing properties of biotransformed asiaticoside by aspergillus niger
publisher	Penerbit UMT
publishDate	2017
url	http://irep.iium.edu.my/60306/ http://irep.iium.edu.my/60306/ http://irep.iium.edu.my/60306/1/PROF%20NO%20JSSM.pdf http://irep.iium.edu.my/60306/9/60306_Wound%20healing%20properties%20of%20biotransformed%20asiaticoside_SCOPUS.pdf
first_indexed	2023-09-18T21:25:29Z
last_indexed	2023-09-18T21:25:29Z
_version_	1777412163022880768

Wound healing properties of biotransformed asiaticoside by aspergillus niger

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