Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts

Anisophyllea disticha has been utilized as traditional medicine to cure diseases such as diarrhea, dysentery, fever, and jaundice. The objectives of this study was to screen the phytochemicals present in A. disticha and to determine the bioactive compounds of A. disticha by using GC-MS. Roots, stems...

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Main Author:	Sahena, Ferdosh
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/60280/ http://irep.iium.edu.my/60280/ http://irep.iium.edu.my/60280/1/Australia%20abstract.pdf

id	iium-60280
recordtype	eprints
spelling	iium-602802017-12-22T06:37:06Z http://irep.iium.edu.my/60280/ Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts Sahena, Ferdosh Q Science (General) Anisophyllea disticha has been utilized as traditional medicine to cure diseases such as diarrhea, dysentery, fever, and jaundice. The objectives of this study was to screen the phytochemicals present in A. disticha and to determine the bioactive compounds of A. disticha by using GC-MS. Roots, stems, and leaves of A. disticha were extracted by Soxhlet extraction using two solvents, methanol, and hexane. Methanol extraction gave a high yield of crude extract compared to hexane extraction. Crude extract from methanol extraction was further used for qualitative phytochemical analysis. Saponin, phenol, tannin and flavonoid were present in the root, stem, and leaves of A. disticha. The steroid was only identified in root and stem of A. disticha. Terpenoid and alkaloid both were absent in roots, stems and leaves of A. disticha. Bioactive compounds of A. disticha were identified by GC-MS. The root of A. disticha consists high amount of Benzenepropanoic acid, 3, 5-bis (1, 1-dimethylethyl)-4-hydroxy-, methylester, followed by 1, 2-Octadecanediol, 1-Nonadecene, 3",8,8"-Trimethoxy-3- Piperidyl-2,2"-binaphthalane-1,1",4,4"-tetrone and 2-Hexadecanol. 1-Octadecanol, behenic alcohol, and 1-Hexadecanol, was major compounds identified in the stem of A. disticha. The leaves of A. disticha was consists mainly 1,6 - Methanonaphthalene-1(2H)-ol,octahydro-4,8a,9,9-tetramethyl-(1a,4β,4aα,6β,8aα) / Patchoulic alcohol, Ethanol, 2-(9-Octadecenyloxy)-(Z), and Hexadecanoic acid, methyl ester / Palauytic ester . Based on antimicrobial assay by disc diffusion method (200, 400 and 600 µg/disc), leaf extracted using methanol exhibited potent growth inhibitory activity against Staphylococcus epidermidis with a diameter of 7.67±0.58 mm.The presence of bioactive compounds of A. disticha proves the potential to treat bacterial diseases. 2017-06-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/60280/1/Australia%20abstract.pdf Sahena, Ferdosh (2017) Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts. In: International Conference on Pharmacology and Regulatory Affairs (ICPR 2017), 12th-14th June 2017, Sydney, Australia. (Unpublished) https://www.cenetrionline.org/speaker/sahena
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sahena, Ferdosh Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
description	Anisophyllea disticha has been utilized as traditional medicine to cure diseases such as diarrhea, dysentery, fever, and jaundice. The objectives of this study was to screen the phytochemicals present in A. disticha and to determine the bioactive compounds of A. disticha by using GC-MS. Roots, stems, and leaves of A. disticha were extracted by Soxhlet extraction using two solvents, methanol, and hexane. Methanol extraction gave a high yield of crude extract compared to hexane extraction. Crude extract from methanol extraction was further used for qualitative phytochemical analysis. Saponin, phenol, tannin and flavonoid were present in the root, stem, and leaves of A. disticha. The steroid was only identified in root and stem of A. disticha. Terpenoid and alkaloid both were absent in roots, stems and leaves of A. disticha. Bioactive compounds of A. disticha were identified by GC-MS. The root of A. disticha consists high amount of Benzenepropanoic acid, 3, 5-bis (1, 1-dimethylethyl)-4-hydroxy-, methylester, followed by 1, 2-Octadecanediol, 1-Nonadecene, 3",8,8"-Trimethoxy-3- Piperidyl-2,2"-binaphthalane-1,1",4,4"-tetrone and 2-Hexadecanol. 1-Octadecanol, behenic alcohol, and 1-Hexadecanol, was major compounds identified in the stem of A. disticha. The leaves of A. disticha was consists mainly 1,6 - Methanonaphthalene-1(2H)-ol,octahydro-4,8a,9,9-tetramethyl-(1a,4β,4aα,6β,8aα) / Patchoulic alcohol, Ethanol, 2-(9-Octadecenyloxy)-(Z), and Hexadecanoic acid, methyl ester / Palauytic ester . Based on antimicrobial assay by disc diffusion method (200, 400 and 600 µg/disc), leaf extracted using methanol exhibited potent growth inhibitory activity against Staphylococcus epidermidis with a diameter of 7.67±0.58 mm.The presence of bioactive compounds of A. disticha proves the potential to treat bacterial diseases.
format	Conference or Workshop Item
author	Sahena, Ferdosh
author_facet	Sahena, Ferdosh
author_sort	Sahena, Ferdosh
title	Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
title_short	Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
title_full	Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
title_fullStr	Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
title_full_unstemmed	Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts
title_sort	phytochemical analysis and antibacterial activity of anisophylliea disticha extracts
publishDate	2017
url	http://irep.iium.edu.my/60280/ http://irep.iium.edu.my/60280/ http://irep.iium.edu.my/60280/1/Australia%20abstract.pdf
first_indexed	2023-09-18T21:25:27Z
last_indexed	2023-09-18T21:25:27Z
_version_	1777412160926777344

Phytochemical analysis and antibacterial activity of Anisophylliea disticha extracts

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