Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract

Context: Syzygium polyanthum (Wight) Walp leaves are traditionally used by Malays for treating hypertension. Our previous study showed that aqueous extract of S. polyanthum (AESP) and methanolic extract of S. polyanthum (MESP) leaves significantly reduced blood pressure of normotensive Wistar‑Kyoto...

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Main Authors:	Ismail, Azlini, Wan Ahmad, Wan Amir Nizam
Format:	Article
Language:	English English
Published:	Wolters Kluwer - Medknow Publications 2017
Subjects:	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/1/PhcogRes_2017_9_5_9_220645.pdf http://irep.iium.edu.my/60211/7/60211_Autonomic%20receptors%20and%20nitric%E2%80%91oxide%20involvements%20in.scopus.pdf

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recordtype	eprints
spelling	iium-602112018-04-05T01:26:41Z http://irep.iium.edu.my/60211/ Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract Ismail, Azlini Wan Ahmad, Wan Amir Nizam RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica Context: Syzygium polyanthum (Wight) Walp leaves are traditionally used by Malays for treating hypertension. Our previous study showed that aqueous extract of S. polyanthum (AESP) and methanolic extract of S. polyanthum (MESP) leaves significantly reduced blood pressure of normotensive Wistar‑Kyoto (WKY) and spontaneously hypertensive rats (SHR). Aims: This study aimed to investigate their vasorelaxation potential and the possible involvement of autonomic receptors and nitric oxide in mediating their effect. Settings and Design: Both extracts will be tested on isolated thoracic aorta rings of WKY and SHR. The involvement of autonomic receptors and nitric oxide will be elucidated using respective blockers. Materials and Methods: Isolated thoracic aorta rings from WKY and SHR were mounted onto myograph chambers to measure changes in the aorta tension. Increasing concentrations of AESP and MESP, from 1 µg/ml to 10 mg/ml were added onto the myograph chambers. Blockers such as atropine (1 µM), phentolamine (1 µM), propranolol (1 µM), and Nω‑nitro‑l‑arginine methyl ester (100 µM) were preincubated before addition of extracts to check for involvement of muscarinic, α‑ and β‑adrenergic receptors (AR) as well as nitric oxide, respectively. Statistical Analysis Used: Two‑way ANOVA, followed by post hoc Bonferroni test was used, where P < 0.05 (two‑tailed) was considered statistically significant. Results: AESP and MESP caused significant vasorelaxations through nitric oxide pathway. The former was mediated through α‑AR while the latter was mediated by β‑adrenergic and muscarinic receptors. Conclusion: Vasorelaxation effect by AESP and MESP involved nitric oxide pathway which is possibly mediated by the autonomic receptors. Wolters Kluwer - Medknow Publications 2017-12-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/60211/1/PhcogRes_2017_9_5_9_220645.pdf application/pdf en http://irep.iium.edu.my/60211/7/60211_Autonomic%20receptors%20and%20nitric%E2%80%91oxide%20involvements%20in.scopus.pdf Ismail, Azlini and Wan Ahmad, Wan Amir Nizam (2017) Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract. Pharmacognosy Research, 9 (Supp 1). S9-S14. ISSN 0976-4836 E-ISSN 0974-8490 http://www.phcogres.com/article.asp?issn=0974-8490;year=2017;volume=9;issue=5;spage=9;epage=14;aulast=Ismail 10.4103/pr.pr_69_17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica
spellingShingle	RM Therapeutics. Pharmacology RM300 Drugs and their action RS Pharmacy and materia medica Ismail, Azlini Wan Ahmad, Wan Amir Nizam Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
description	Context: Syzygium polyanthum (Wight) Walp leaves are traditionally used by Malays for treating hypertension. Our previous study showed that aqueous extract of S. polyanthum (AESP) and methanolic extract of S. polyanthum (MESP) leaves significantly reduced blood pressure of normotensive Wistar‑Kyoto (WKY) and spontaneously hypertensive rats (SHR). Aims: This study aimed to investigate their vasorelaxation potential and the possible involvement of autonomic receptors and nitric oxide in mediating their effect. Settings and Design: Both extracts will be tested on isolated thoracic aorta rings of WKY and SHR. The involvement of autonomic receptors and nitric oxide will be elucidated using respective blockers. Materials and Methods: Isolated thoracic aorta rings from WKY and SHR were mounted onto myograph chambers to measure changes in the aorta tension. Increasing concentrations of AESP and MESP, from 1 µg/ml to 10 mg/ml were added onto the myograph chambers. Blockers such as atropine (1 µM), phentolamine (1 µM), propranolol (1 µM), and Nω‑nitro‑l‑arginine methyl ester (100 µM) were preincubated before addition of extracts to check for involvement of muscarinic, α‑ and β‑adrenergic receptors (AR) as well as nitric oxide, respectively. Statistical Analysis Used: Two‑way ANOVA, followed by post hoc Bonferroni test was used, where P < 0.05 (two‑tailed) was considered statistically significant. Results: AESP and MESP caused significant vasorelaxations through nitric oxide pathway. The former was mediated through α‑AR while the latter was mediated by β‑adrenergic and muscarinic receptors. Conclusion: Vasorelaxation effect by AESP and MESP involved nitric oxide pathway which is possibly mediated by the autonomic receptors.
format	Article
author	Ismail, Azlini Wan Ahmad, Wan Amir Nizam
author_facet	Ismail, Azlini Wan Ahmad, Wan Amir Nizam
author_sort	Ismail, Azlini
title	Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
title_short	Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
title_full	Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
title_fullStr	Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
title_full_unstemmed	Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
title_sort	autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract
publisher	Wolters Kluwer - Medknow Publications
publishDate	2017
url	http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/ http://irep.iium.edu.my/60211/1/PhcogRes_2017_9_5_9_220645.pdf http://irep.iium.edu.my/60211/7/60211_Autonomic%20receptors%20and%20nitric%E2%80%91oxide%20involvements%20in.scopus.pdf
first_indexed	2023-09-18T21:25:21Z
last_indexed	2023-09-18T21:25:21Z
_version_	1777412154826162176

Autonomic receptors and nitric‑oxide involvements in mediating vasorelaxation effect induced by syzygium polyanthum leaves extract

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