Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite

Introduction: The objective of this study was to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing staphylococcus aureus through an in vitro experimental setting. Our hypothesis was that orthopaedic implant coated with silver would exert both bacte...

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Main Authors:	Mohd Jan, Nurul Hafiza, Zulkifly, Ahmad Hafiz, Ibrahim, Mohd Zulfadzli, Sha'ban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	QR Microbiology RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/60163/ http://irep.iium.edu.my/60163/ http://irep.iium.edu.my/60163/1/60163_Initial%20Response%20in%20Antibacterial%20Activity.pdf

id	iium-60163
recordtype	eprints
spelling	iium-601632017-12-15T02:46:40Z http://irep.iium.edu.my/60163/ Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafiz Ibrahim, Mohd Zulfadzli Sha'ban, Munirah QR Microbiology RD701 Orthopedics Introduction: The objective of this study was to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing staphylococcus aureus through an in vitro experimental setting. Our hypothesis was that orthopaedic implant coated with silver would exert both bacteriostatic and bactericidal effects against staphylococcus aureus. Materials & methods: The silver coated plate was soaked with 200ml of phosphate buffered saline (PBS) in shaking water bath at 37˚C for silver release preparation. 5.0ml solution was collected at 1-, 3-, 24-, 48-, 168-, 336- and 504-hour time point for analyses. Bacterial inoculation was smeared on sterile nutrient agar media. Plain discs placement were loaded on the surface of nutrient agar. 20µl silver release solution in each assessment was loaded on the plain disc. 20µl of PBS also was loaded on the plain disc and used as control. All samples were incubated at 37 ˚C for 24 hours for bacterial culture under aerobic condition. The samples were incubated up to 168 hours under the same conditions for assessment. The antibacterial effect was evaluated based on the diameter of inhibition zone using disc diffusion test. Results and Discussions: Bacterial colony was noted on the surface of nutrient agar after 24 hours incubation. However, the inhibition zone with a diameter more than 20mm was noted at 168 hours of silver release after 168 hours incubation. The outcome of this study revealed positive indicator of the silver as antibacterial agent. Conclusion: This result suggested that orthopaedic metal implant coated with silver composite may provide antibacterial protection against medical device-related infection. 2017-11-21 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/60163/1/60163_Initial%20Response%20in%20Antibacterial%20Activity.pdf Mohd Jan, Nurul Hafiza and Zulkifly, Ahmad Hafiz and Ibrahim, Mohd Zulfadzli and Sha'ban, Munirah (2017) Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite. In: Fundamental Science Congress 2017 (FSC2017), 21st-22nd Nov. 2017, Serdang, Selangor. (Unpublished) http://www.fsc2017.com/index.php/2013-03-08-03-26-20/poster-presentation-list
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology RD701 Orthopedics
spellingShingle	QR Microbiology RD701 Orthopedics Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafiz Ibrahim, Mohd Zulfadzli Sha'ban, Munirah Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
description	Introduction: The objective of this study was to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing staphylococcus aureus through an in vitro experimental setting. Our hypothesis was that orthopaedic implant coated with silver would exert both bacteriostatic and bactericidal effects against staphylococcus aureus. Materials & methods: The silver coated plate was soaked with 200ml of phosphate buffered saline (PBS) in shaking water bath at 37˚C for silver release preparation. 5.0ml solution was collected at 1-, 3-, 24-, 48-, 168-, 336- and 504-hour time point for analyses. Bacterial inoculation was smeared on sterile nutrient agar media. Plain discs placement were loaded on the surface of nutrient agar. 20µl silver release solution in each assessment was loaded on the plain disc. 20µl of PBS also was loaded on the plain disc and used as control. All samples were incubated at 37 ˚C for 24 hours for bacterial culture under aerobic condition. The samples were incubated up to 168 hours under the same conditions for assessment. The antibacterial effect was evaluated based on the diameter of inhibition zone using disc diffusion test. Results and Discussions: Bacterial colony was noted on the surface of nutrient agar after 24 hours incubation. However, the inhibition zone with a diameter more than 20mm was noted at 168 hours of silver release after 168 hours incubation. The outcome of this study revealed positive indicator of the silver as antibacterial agent. Conclusion: This result suggested that orthopaedic metal implant coated with silver composite may provide antibacterial protection against medical device-related infection.
format	Conference or Workshop Item
author	Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafiz Ibrahim, Mohd Zulfadzli Sha'ban, Munirah
author_facet	Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafiz Ibrahim, Mohd Zulfadzli Sha'ban, Munirah
author_sort	Mohd Jan, Nurul Hafiza
title	Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
title_short	Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
title_full	Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
title_fullStr	Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
title_full_unstemmed	Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
title_sort	initial response in antibacterial activity of orthopaedic metal implant coated with silver composite
publishDate	2017
url	http://irep.iium.edu.my/60163/ http://irep.iium.edu.my/60163/ http://irep.iium.edu.my/60163/1/60163_Initial%20Response%20in%20Antibacterial%20Activity.pdf
first_indexed	2023-09-18T21:25:16Z
last_indexed	2023-09-18T21:25:16Z
_version_	1777412149810823168

Initial response in antibacterial activity of orthopaedic metal implant coated with silver composite

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