Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers

Plants of the Rutaceae family are sources of active alkaloids with high potential to defeat microbial infectious diseases and overcome microbial resistance towards antibiotic. The anthranilic acid derived alkaloid especially quinoline, furoquinoline, acridone and 4-quinolone alkaloids are the common...

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Main Authors:	Mohd Kamal, Laina Zarisa, Mohd Hassan, Norazian, Md Taib, Nurhaya, May Khin, Soe
Format:	Conference or Workshop Item
Language:	English
Published:	2010
Subjects:	QD Chemistry QK Botany QR180 Immunology RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
Online Access:	http://irep.iium.edu.my/6014/ http://irep.iium.edu.my/6014/ http://irep.iium.edu.my/6014/1/POSTER.pdf

id	iium-6014
recordtype	eprints
spelling	iium-60142011-11-21T21:42:37Z http://irep.iium.edu.my/6014/ Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya May Khin, Soe QD Chemistry QK Botany QR180 Immunology RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Plants of the Rutaceae family are sources of active alkaloids with high potential to defeat microbial infectious diseases and overcome microbial resistance towards antibiotic. The anthranilic acid derived alkaloid especially quinoline, furoquinoline, acridone and 4-quinolone alkaloids are the common types of antimicrobial active alkaloids found in Rutaceae. In this study bioautography-directed fractionation of the crude alkaloidal extract from Ruta angustifolia (L.) Pers. was carried out to focus on the isolation of the antimicrobial active alkaloids. The isolated alkaloids were further identified by spectroscopic method of NMR. The activity of each isolated antimicrobial active alkaloid was quantified by using broth microdilution assay for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 25933, Pseudomonas aeruginosa ATCC 27853, Helicobacter pylori ATCC 43504, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 90028. Two promising antimicrobial alkaloids which are an acridone, arborinine and a 4-quinolone, graveoline were isolated. Arborinine showed an activity against S. aureus, E. coli, P. aeruginosa and C. albicans while graveoline exhibits an activity against all of the bacteria except E. faecalis. MIC values for the two alkaloids against the susceptible microbes ranged between 0.01 mg/mL and 0.025 mg/mL while the MBC values were more than 0.01 mg/mL. These results suggest that the two compounds could be considered as a source of antimicrobial agent which might be studied in more details. 2010-10-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/6014/1/POSTER.pdf Mohd Kamal, Laina Zarisa and Mohd Hassan, Norazian and Md Taib, Nurhaya and May Khin, Soe (2010) Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers. In: 3rd International Conference on Biotechnology for the Wellness Industry & Exhibition, 8-9 October 2010, Putra World Trade Centre, Kuala Lumpur, Malaysia. http://www.cepp.utm.my/icbwi10
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry QK Botany QR180 Immunology RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine
spellingShingle	QD Chemistry QK Botany QR180 Immunology RS Pharmacy and materia medica RV Botanic, Thomsonian, and eclectic medicine Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya May Khin, Soe Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
description	Plants of the Rutaceae family are sources of active alkaloids with high potential to defeat microbial infectious diseases and overcome microbial resistance towards antibiotic. The anthranilic acid derived alkaloid especially quinoline, furoquinoline, acridone and 4-quinolone alkaloids are the common types of antimicrobial active alkaloids found in Rutaceae. In this study bioautography-directed fractionation of the crude alkaloidal extract from Ruta angustifolia (L.) Pers. was carried out to focus on the isolation of the antimicrobial active alkaloids. The isolated alkaloids were further identified by spectroscopic method of NMR. The activity of each isolated antimicrobial active alkaloid was quantified by using broth microdilution assay for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 25933, Pseudomonas aeruginosa ATCC 27853, Helicobacter pylori ATCC 43504, Enterococcus faecalis ATCC 29212 and Candida albicans ATCC 90028. Two promising antimicrobial alkaloids which are an acridone, arborinine and a 4-quinolone, graveoline were isolated. Arborinine showed an activity against S. aureus, E. coli, P. aeruginosa and C. albicans while graveoline exhibits an activity against all of the bacteria except E. faecalis. MIC values for the two alkaloids against the susceptible microbes ranged between 0.01 mg/mL and 0.025 mg/mL while the MBC values were more than 0.01 mg/mL. These results suggest that the two compounds could be considered as a source of antimicrobial agent which might be studied in more details.
format	Conference or Workshop Item
author	Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya May Khin, Soe
author_facet	Mohd Kamal, Laina Zarisa Mohd Hassan, Norazian Md Taib, Nurhaya May Khin, Soe
author_sort	Mohd Kamal, Laina Zarisa
title	Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
title_short	Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
title_full	Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
title_fullStr	Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
title_full_unstemmed	Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers
title_sort	bioassay-guided isolation of antimicrobial active alkaloids from ruta angustifolia (l.) pers
publishDate	2010
url	http://irep.iium.edu.my/6014/ http://irep.iium.edu.my/6014/ http://irep.iium.edu.my/6014/1/POSTER.pdf
first_indexed	2023-09-18T20:14:50Z
last_indexed	2023-09-18T20:14:50Z
_version_	1777407717995970560

Bioassay-guided isolation of antimicrobial active alkaloids from Ruta angustifolia (L.) Pers

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