Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption. Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase...

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Main Authors:	Doolaanea, Abd Almonem, Mansor, Nur 'Izzati, Nor, Nurul Hafizah Mohd, Mohd Shafri, Mohd Affendi, Susi, Sukmasari, Mohamed, Farahidah
Format:	Article
Language:	English English
Published:	Faculty of Pharmacy, University of Benin, Benin City, Nigeria 2017
Subjects:	RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/2/Comparative%20assessment%20of%20poly%20%28D%2CL-lactide-co-glycolide%29.pdf http://irep.iium.edu.my/60006/8/Comparative%20assessment%20of%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles%20modified%20by%20either%20cetyltrimethylammonium%20bromide%20or%20chitosan%20for%20plasmid%20DNA%20adsorption.pdf

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spelling	iium-600062018-07-10T09:34:50Z http://irep.iium.edu.my/60006/ Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption Doolaanea, Abd Almonem Mansor, Nur 'Izzati Nor, Nurul Hafizah Mohd Mohd Shafri, Mohd Affendi Susi, Sukmasari Mohamed, Farahidah RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption. Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection. Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection. Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity. Faculty of Pharmacy, University of Benin, Benin City, Nigeria 2017-10 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/60006/2/Comparative%20assessment%20of%20poly%20%28D%2CL-lactide-co-glycolide%29.pdf application/pdf en http://irep.iium.edu.my/60006/8/Comparative%20assessment%20of%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles%20modified%20by%20either%20cetyltrimethylammonium%20bromide%20or%20chitosan%20for%20plasmid%20DNA%20adsorption.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Nor, Nurul Hafizah Mohd and Mohd Shafri, Mohd Affendi and Susi, Sukmasari and Mohamed, Farahidah (2017) Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption. Tropical Journal of Pharmaceutical Research, 16 (10). pp. 2315-2324. ISSN 1596-5996 E-ISSN 1596-9827 https://www.ajol.info/index.php/tjpr/article/view/162860 10.4314/tjpr.v16i10.2
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology RS403 Materia Medica-Pharmaceutical Chemistry Doolaanea, Abd Almonem Mansor, Nur 'Izzati Nor, Nurul Hafizah Mohd Mohd Shafri, Mohd Affendi Susi, Sukmasari Mohamed, Farahidah Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
description	Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption. Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection. Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection. Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.
format	Article
author	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Nor, Nurul Hafizah Mohd Mohd Shafri, Mohd Affendi Susi, Sukmasari Mohamed, Farahidah
author_facet	Doolaanea, Abd Almonem Mansor, Nur 'Izzati Nor, Nurul Hafizah Mohd Mohd Shafri, Mohd Affendi Susi, Sukmasari Mohamed, Farahidah
author_sort	Doolaanea, Abd Almonem
title	Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
title_short	Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
title_full	Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
title_fullStr	Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
title_full_unstemmed	Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
title_sort	comparative assessment of poly (d, l-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid dna adsorption
publisher	Faculty of Pharmacy, University of Benin, Benin City, Nigeria
publishDate	2017
url	http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/2/Comparative%20assessment%20of%20poly%20%28D%2CL-lactide-co-glycolide%29.pdf http://irep.iium.edu.my/60006/8/Comparative%20assessment%20of%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles%20modified%20by%20either%20cetyltrimethylammonium%20bromide%20or%20chitosan%20for%20plasmid%20DNA%20adsorption.pdf
first_indexed	2023-09-18T21:25:03Z
last_indexed	2023-09-18T21:25:03Z
_version_	1777412135903559680

Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

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