Comparative assessment of poly (D, L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption
Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption. Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Faculty of Pharmacy, University of Benin, Benin City, Nigeria
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/ http://irep.iium.edu.my/60006/2/Comparative%20assessment%20of%20poly%20%28D%2CL-lactide-co-glycolide%29.pdf http://irep.iium.edu.my/60006/8/Comparative%20assessment%20of%20poly%20%28D%2C%20L-lactide-co-glycolide%29%20nanoparticles%20modified%20by%20either%20cetyltrimethylammonium%20bromide%20or%20chitosan%20for%20plasmid%20DNA%20adsorption.pdf |
| Summary: | Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl
ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption.
Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including
CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight
chitosan. The nanoparticles were characterised by differential scanning calorimetry (DSC) and Fourier
transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and transfection.
Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1
surface. This resulted in negative zeta potential, increased cytotoxicity as well as decreased cell uptake
and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with
decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand,
chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA,
leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and
transfection. Amongst the four formulations, F4 exhibited the highest transfection.
Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB
and chitosan. Modification with chitosan yields PLGA nanoparticles with higher DNA adsorption and
transfection with lower cytotoxicity. |
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