Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)

Immobilization of lipase enzyme originated from wheat germ on nylon -6- grafted with PGMA was studied. Face centered central composite design (FCCCD) under response surface methodology (RSM) was used to design the experiment in order to distinguish the optimum immobilization conditions for highest e...

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Main Authors:	Alkhatib, Ma'an Fahmi Rashid, Bahrudin, Nik Adlin, Mohd. Salleh, Hamzah, Nasef, Mohamed Mahmoud
Format:	Conference or Workshop Item
Language:	English
Published:	IMDD 2017
Subjects:	TA164 Bioengineering TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/59822/ http://irep.iium.edu.my/59822/ http://irep.iium.edu.my/59822/1/59822_Response%20Surface%20Methodology.pdf

id	iium-59822
recordtype	eprints
spelling	iium-598222017-12-07T04:02:59Z http://irep.iium.edu.my/59822/ Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA) Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud TA164 Bioengineering TP155 Chemical engineering Immobilization of lipase enzyme originated from wheat germ on nylon -6- grafted with PGMA was studied. Face centered central composite design (FCCCD) under response surface methodology (RSM) was used to design the experiment in order to distinguish the optimum immobilization conditions for highest enzyme activity and to study the interactions between the various parameters. The polymer was activated with diethyl amine/ethanol to introduce amine functional group to facilitate covalent bonding with the enzyme. The immobilization conditions were 2-10 h for immobilization time; 6-8 for pH and 0.4-1.0 mg/ml for enzyme concentration. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. Kinetics studies of both immobilized and free lipase were conducted to study the effect of immobilization on enzyme behavior. Amongst the tested models, Lineweaver-Burk Plot best fitted the data with Vmax and Km values of 0.024 mM/min and 10.321 mM for free lipase and 0.012 and 7.250 for immobilized lipase, respectively. The lower value of Km for immobilized lipase enzyme compared to free enzyme indicates a stronger and more efficient binding of substrate. Vmax on the other hand exemplify how quick the enzyme catalyzes the reaction. Lower Vmax for immobilized enzyme designates lower enzyme rate. IMDD 2017-11-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59822/1/59822_Response%20Surface%20Methodology.pdf Alkhatib, Ma'an Fahmi Rashid and Bahrudin, Nik Adlin and Mohd. Salleh, Hamzah and Nasef, Mohamed Mahmoud (2017) Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA). In: 3rd International Symposium on Materials and Sustainable Development (ISMSD2017), 7th-8th November 2017, Boumerdes, Algeria. https://cimdd2017.sciencesconf.org/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering TP155 Chemical engineering
spellingShingle	TA164 Bioengineering TP155 Chemical engineering Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
description	Immobilization of lipase enzyme originated from wheat germ on nylon -6- grafted with PGMA was studied. Face centered central composite design (FCCCD) under response surface methodology (RSM) was used to design the experiment in order to distinguish the optimum immobilization conditions for highest enzyme activity and to study the interactions between the various parameters. The polymer was activated with diethyl amine/ethanol to introduce amine functional group to facilitate covalent bonding with the enzyme. The immobilization conditions were 2-10 h for immobilization time; 6-8 for pH and 0.4-1.0 mg/ml for enzyme concentration. ANOVA revealed that optimum lipase activity of 0.287 U/ml was achieved at immobilization time of 5 h, pH of 6 and 1.0 mg/ml for enzyme concentration. Kinetics studies of both immobilized and free lipase were conducted to study the effect of immobilization on enzyme behavior. Amongst the tested models, Lineweaver-Burk Plot best fitted the data with Vmax and Km values of 0.024 mM/min and 10.321 mM for free lipase and 0.012 and 7.250 for immobilized lipase, respectively. The lower value of Km for immobilized lipase enzyme compared to free enzyme indicates a stronger and more efficient binding of substrate. Vmax on the other hand exemplify how quick the enzyme catalyzes the reaction. Lower Vmax for immobilized enzyme designates lower enzyme rate.
format	Conference or Workshop Item
author	Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud
author_facet	Alkhatib, Ma'an Fahmi Rashid Bahrudin, Nik Adlin Mohd. Salleh, Hamzah Nasef, Mohamed Mahmoud
author_sort	Alkhatib, Ma'an Fahmi Rashid
title	Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
title_short	Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
title_full	Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
title_fullStr	Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
title_full_unstemmed	Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)
title_sort	response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (pgma)
publisher	IMDD
publishDate	2017
url	http://irep.iium.edu.my/59822/ http://irep.iium.edu.my/59822/ http://irep.iium.edu.my/59822/1/59822_Response%20Surface%20Methodology.pdf
first_indexed	2023-09-18T21:24:47Z
last_indexed	2023-09-18T21:24:47Z
_version_	1777412119279435776

Response surface methodology for optimization of lipase immobilization conditions on nanofibers grafted with polyglycidyl methachrylate (PGMA)

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