Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study

Introduction: Evidence suggests that several genes; including MYO1H, play an important role in the etiology of Class III malocclusion. Single nucleotide polymorphism (SNP) in marker rs10850110 (locus 12q24.11) within MYO1H gene has been associated with the incidence of mandibular prognathism (MP). M...

Full description

Bibliographic Details
Main Authors:	Yahya, Siti Nazirah, Abdul Razak, Nurul Syafiqah, Abu Bakar, Noraini, Mokhtar@Makhtar, Khairani Idah, Kharuddin, Azrul Fazwan
Format:	Article
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/59808/ http://irep.iium.edu.my/59808/ http://irep.iium.edu.my/59808/7/59808-Analysis%20Of%20MYO1H%20Single%20Nucleotide%20Polymorphism.pdf

id	iium-59808
recordtype	eprints
spelling	iium-598082017-12-26T07:07:06Z http://irep.iium.edu.my/59808/ Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study Yahya, Siti Nazirah Abdul Razak, Nurul Syafiqah Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan RK Dentistry Introduction: Evidence suggests that several genes; including MYO1H, play an important role in the etiology of Class III malocclusion. Single nucleotide polymorphism (SNP) in marker rs10850110 (locus 12q24.11) within MYO1H gene has been associated with the incidence of mandibular prognathism (MP). MYO is a class 1 myosin that is responsible for the synthesis of Matrilin-1; an important protein involved in the formation of cartilage's extracellular matrix, hence is implicated in the formation of mandibular condyle cartilage. This study aimed to detect the presence of MYO1H (rs10850110) SNP and to determine its genotype and allele distribution in MP patient in the local population. Materials and Methods: The sample comprises of 31 patients; 14 patients from class I malocclusion (control samples) and 17 patients from class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs10850110 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of MYO1H SNP (rs10850110) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): Our findings are in agreement with previous studies suggesting positive influence of MYO1H (rs10850110) SNP in the incidence of MP. Further studies should be developed in order to understand the exact role and mechanism of MYO1H in different classes of malocclusions. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59808/7/59808-Analysis%20Of%20MYO1H%20Single%20Nucleotide%20Polymorphism.pdf Yahya, Siti Nazirah and Abdul Razak, Nurul Syafiqah and Abu Bakar, Noraini and Mokhtar@Makhtar, Khairani Idah and Kharuddin, Azrul Fazwan (2017) Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study. The International Medical Journal of Malaysia, 16 (Supplementary Issue No 2). p. 1. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%202/ID15_IMJM-Vol16-Supp2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Yahya, Siti Nazirah Abdul Razak, Nurul Syafiqah Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
description	Introduction: Evidence suggests that several genes; including MYO1H, play an important role in the etiology of Class III malocclusion. Single nucleotide polymorphism (SNP) in marker rs10850110 (locus 12q24.11) within MYO1H gene has been associated with the incidence of mandibular prognathism (MP). MYO is a class 1 myosin that is responsible for the synthesis of Matrilin-1; an important protein involved in the formation of cartilage's extracellular matrix, hence is implicated in the formation of mandibular condyle cartilage. This study aimed to detect the presence of MYO1H (rs10850110) SNP and to determine its genotype and allele distribution in MP patient in the local population. Materials and Methods: The sample comprises of 31 patients; 14 patients from class I malocclusion (control samples) and 17 patients from class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs10850110 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of MYO1H SNP (rs10850110) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): Our findings are in agreement with previous studies suggesting positive influence of MYO1H (rs10850110) SNP in the incidence of MP. Further studies should be developed in order to understand the exact role and mechanism of MYO1H in different classes of malocclusions.
format	Article
author	Yahya, Siti Nazirah Abdul Razak, Nurul Syafiqah Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan
author_facet	Yahya, Siti Nazirah Abdul Razak, Nurul Syafiqah Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan
author_sort	Yahya, Siti Nazirah
title	Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
title_short	Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
title_full	Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
title_fullStr	Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
title_full_unstemmed	Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study
title_sort	analysis of myo1h single nucleotide polymorphism in class iii malocclusion with mandibular prognathism: a preliminary study
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/59808/ http://irep.iium.edu.my/59808/ http://irep.iium.edu.my/59808/7/59808-Analysis%20Of%20MYO1H%20Single%20Nucleotide%20Polymorphism.pdf
first_indexed	2023-09-18T21:24:45Z
last_indexed	2023-09-18T21:24:45Z
_version_	1777412117345861632

Analysis Of MYO1H single nucleotide polymorphism in class III malocclusion with mandibular prognathism: A preliminary study

Similar Items