Electrochemical characterization of polylactic acid-block-poly (2-vinylpyridine)/gold nanoparticle composites for glucose biosensor development
Nanocomposites that consist of diblock copolymer (BCP) and gold nanoparticles (AuNPs) can be applied as a matrix to immobilize enzymes or other molecules, based on the well-defined core/shell nanostructures of these composites. In this research, polylactic acid-block-poly(2-vinylpyridine) (PLA-b-...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia (IIUM)
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/59806/ http://irep.iium.edu.my/59806/ http://irep.iium.edu.my/59806/7/59806-Electrochemical%20characterization%20of%20polylactic%20acid-block-poly.pdf http://irep.iium.edu.my/59806/13/Electrochemical%20characterization%20of%20polylactic%20acid-block-poly%20%282-vinylpyridine%29%20gold%20nanoparticle%20composites%20for%20glucose%20biosensor%20development.pdf |
| Summary: | Nanocomposites that consist of diblock copolymer (BCP) and gold
nanoparticles (AuNPs) can be applied as a matrix to immobilize enzymes or other
molecules, based on the well-defined core/shell nanostructures of these composites. In
this research, polylactic acid-block-poly(2-vinylpyridine) (PLA-b-P2VP)/hydrogen
tetrachloroaurate(III) hydrate (HAuCl4.3H2O) composites were hybridized and then
reduced in dichloromethane (DCM) solution. The hybridizations between gold
precursors and the P2VP domain were prepared with different ratios of gold to P2VP
block (1:1, 1:5, 1:10, 5:1, 10:1) by taking advantage of the association between the longpair
nitrogen of the pyridine group of P2VP. The reduction of the Au3+/PLA-b-P2VP
composite was accomplished by hydrazine solution in order to get gold
nanoparticle/PLA-b-P2VP composites, which was visually confirmed by a direct color
change from bright yellow to purple. In this work, ultraviolet–visible (UV-vis)
spectroscopy and Fourier transform infrared spectroscopy (FTIR) were used to confirm
the association between gold precursors and pyridine groups as well as the synthesis of
gold nanoparticles. The composite which labeled as R3 (Au3+: P2VP = 10:1) showed the
highest peak current based on the cyclic voltammetry (CV) measurement. Furthermore,
graphene oxide (GO) was added into R3 to prepare BCP/AuNPs/GO composite and
reduced to BCP/AuNPs/rGO through electrochemical reduction. The resulting
BCP/AuNPs/rGO showed high potential to be used in amperometric biosensor
Nanokomposit yang terdiri daripada diblok kopolimer (BCP) dan
nanopartikel emas (AuNPs) boleh digunakan sebagai matrik untuk memegun enzim atau
molekul lain berdasarkan struktur asas nano komposit. Dalam kajian ini, polilaktik asidblok-poli(2-vinylpyridine) (PLA-b-P2VP)/hidrogen tetrachloroaurate (III) hidrat
(HAuCl4.3H2O) komposit telah dihibrid dan kemudian direduksi dalam larutan
diklorometana (DCM). Proses hibridasi antara prekursor emas dan domain P2VP
dilakukan dengan nisbah yang berbeza; emas kepada blok P2VP (1:1, 1:5, 1:10, 5:1,
10:1) dengan mengambil peluang daripada gabungan antara kumpulan nitrogen dan
kumpulan P2VP piridina. Reduksi komposit Au
3+/ PLA-b-P2VP dapat dihasilkan dengan
larutan hidrazin bagi mendapatkan komposit nanopartikel emas/PLA-b-P2VP, ini
disahkan dengan perubahan warna daripada kuning kepada ungu. Dalam kajian ini,
kehadiran-ultraungu spektroskopi (UV-vis) dan perubahan Fourier inframerah (FTIR)
telah digunakan untuk mengesahkan hubungan antara prekursor emas dan kumpulan
piridina serta sintesis emas nanopartikel. Komposit yang dilabelkan sebagai R3 (Au3+ :
P2VP = 10:1) menunjukkan puncak tertinggi arus berdasarkan nilai kitaran voltammetri (CV). Tambahan, graphene oksida (GO) telah ditambah ke dalam R3 untuk menyediakan
komposit BCP/AuNPs/GO dan menukarkan kepada BCP/AuNPs/rGO melalui teknik
penurunan elektrokimia. Keputusan komposit BCP/AuNPs/rGO menunjukkan potensi
yang tinggi untuk digunakan dalam biosensor amperometrik. |
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