Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...

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Main Authors:	Samsudin, Nurhusna, Hashim, Yumi Zuhanis Has-Yun, Arifin, Mohd Azmir, Mel, Maizirwan, Mohd. Salleh, Hamzah, Nordin, Norshariza, Sopyan, Iis, Jimat, Dzun Noraini
Format:	Article
Language:	English English
Published:	Penerbit UTM Press 2017
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/59803/ http://irep.iium.edu.my/59803/ http://irep.iium.edu.my/59803/1/JurTek%202017.pdf http://irep.iium.edu.my/59803/7/59803-Statistical%20optimization%20of%20gelatin%20immobilisation.pdf

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spelling	iium-598032018-05-17T07:20:39Z http://irep.iium.edu.my/59803/ Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini TA164 Bioengineering Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 μg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105cells/ml) and UV/O3 treated PCL microcarrier (4.25×105cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. Penerbit UTM Press 2017-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59803/1/JurTek%202017.pdf application/pdf en http://irep.iium.edu.my/59803/7/59803-Statistical%20optimization%20of%20gelatin%20immobilisation.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Nordin, Norshariza and Sopyan, Iis and Jimat, Dzun Noraini (2017) Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility. Jurnal Teknologi, 79 (6). pp. 167-175. ISSN 2180-3722 http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/10417/6222
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TA164 Bioengineering
spellingShingle	TA164 Bioengineering Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
description	Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 μg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105cells/ml) and UV/O3 treated PCL microcarrier (4.25×105cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier.
format	Article
author	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini
author_facet	Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Nordin, Norshariza Sopyan, Iis Jimat, Dzun Noraini
author_sort	Samsudin, Nurhusna
title	Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_short	Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_full	Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_fullStr	Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_full_unstemmed	Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility
title_sort	statistical optimization of gelatin immobilization on modified surface pcl microcarrier to improve pcl microcarrier compatibility
publisher	Penerbit UTM Press
publishDate	2017
url	http://irep.iium.edu.my/59803/ http://irep.iium.edu.my/59803/ http://irep.iium.edu.my/59803/1/JurTek%202017.pdf http://irep.iium.edu.my/59803/7/59803-Statistical%20optimization%20of%20gelatin%20immobilisation.pdf
first_indexed	2023-09-18T21:24:45Z
last_indexed	2023-09-18T21:24:45Z
_version_	1777412116591935488

Statistical optimization of gelatin immobilization on modified surface PCL microcarrier to improve PCL microcarrier compatibility

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