Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)

Introduction: Flaxseeds offer a wide range of pharmacological properties including antioxidant, antibacterial and anticancer. However its effect on mesenchymal stem cells has not been elucidated. Thus, this study aimed to determine the effects of flaxseed crude extract on stem cell from human exfoli...

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Main Authors:	Nordin, Nur Sazwi, Md. Isa, Muhammad Lokman, Lestari, Widya, Mustafa Al-Ahmad, Basma Ezzat, Arief Ichwan, Solachuddin Jauhari, Darnis, Deny Susanti, Thirumulu Ponnuraj, Kannan, Ahmad, Azlina, Mokhtar@Makhtar, Khairani Idah
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2017
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/59676/ http://irep.iium.edu.my/59676/ http://irep.iium.edu.my/59676/1/Nursazwi%20et%20al-2017%20IIDC-IMJM%20Vol%2016%20Supp%202.pdf

id	iium-59676
recordtype	eprints
spelling	iium-596762017-11-29T07:24:04Z http://irep.iium.edu.my/59676/ Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED) Nordin, Nur Sazwi Md. Isa, Muhammad Lokman Lestari, Widya Mustafa Al-Ahmad, Basma Ezzat Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah RK Dentistry Introduction: Flaxseeds offer a wide range of pharmacological properties including antioxidant, antibacterial and anticancer. However its effect on mesenchymal stem cells has not been elucidated. Thus, this study aimed to determine the effects of flaxseed crude extract on stem cell from human exfoliated deciduous teeth (SHED) in terms of cell viability, morphology and proliferation activity. Materials and Methods: Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed on SHED were assessed for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamar Blue assay. Fatty acid composition of flaxseed was analysed using gas chromatography-mass spectrometry (GCMS) instrumental technique. Results: The effects of flaxseed on SHED were observed to be dose-dependent, where higher concentration of the extract resulted in lower cell viability. The concentration of the flaxseed required to inhibit 50% of cell viability (IC50) was 10.56±0.22 mg/ml. Morphological observation demonstrated that flaxseed altered the cell morphology at concentration above 8 mg/ml. Based on alamarBlue assay, SHED treated with flaxseed at concentration 0.5, 1, 2, 4, 8, 10.56 mg/ml showed no significant difference of PDT when compared to control (p>.05). GCMS analysis revealed the presence of linolenic acid as major compound, linoleic acid and palmitic acid and oleic acid. Conclusion(s): Crude extract of flaxseed at concentration below 8 mg/ml may be applied in the future study of SHED. The linolenic acid in flaxseed may have been responsible for the cell viability and proliferation activity of SHED. Kulliyyah of Medicine, International Islamic University Malaysia 2017-11-13 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59676/1/Nursazwi%20et%20al-2017%20IIDC-IMJM%20Vol%2016%20Supp%202.pdf Nordin, Nur Sazwi and Md. Isa, Muhammad Lokman and Lestari, Widya and Mustafa Al-Ahmad, Basma Ezzat and Arief Ichwan, Solachuddin Jauhari and Darnis, Deny Susanti and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina and Mokhtar@Makhtar, Khairani Idah (2017) Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED). In: 1st IIUM International Dental Conference 2017 in Commemoration of the Kulliyyah of Dentistry 10th Anniversary (1st IIDC), 6th-8th October 2017, Kuantan, Pahang. http://iiumedic.net/imjm/v1/about-imjm/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Nordin, Nur Sazwi Md. Isa, Muhammad Lokman Lestari, Widya Mustafa Al-Ahmad, Basma Ezzat Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
description	Introduction: Flaxseeds offer a wide range of pharmacological properties including antioxidant, antibacterial and anticancer. However its effect on mesenchymal stem cells has not been elucidated. Thus, this study aimed to determine the effects of flaxseed crude extract on stem cell from human exfoliated deciduous teeth (SHED) in terms of cell viability, morphology and proliferation activity. Materials and Methods: Whole flaxseeds were ground and extracted with absolute ethanol using soxhlet extractor. The effects of flaxseed on SHED were assessed for cell viability using MTT assay, cell morphology using inverted microscope and proliferative activity describe as population doubling time (PDT) using alamar Blue assay. Fatty acid composition of flaxseed was analysed using gas chromatography-mass spectrometry (GCMS) instrumental technique. Results: The effects of flaxseed on SHED were observed to be dose-dependent, where higher concentration of the extract resulted in lower cell viability. The concentration of the flaxseed required to inhibit 50% of cell viability (IC50) was 10.56±0.22 mg/ml. Morphological observation demonstrated that flaxseed altered the cell morphology at concentration above 8 mg/ml. Based on alamarBlue assay, SHED treated with flaxseed at concentration 0.5, 1, 2, 4, 8, 10.56 mg/ml showed no significant difference of PDT when compared to control (p>.05). GCMS analysis revealed the presence of linolenic acid as major compound, linoleic acid and palmitic acid and oleic acid. Conclusion(s): Crude extract of flaxseed at concentration below 8 mg/ml may be applied in the future study of SHED. The linolenic acid in flaxseed may have been responsible for the cell viability and proliferation activity of SHED.
format	Conference or Workshop Item
author	Nordin, Nur Sazwi Md. Isa, Muhammad Lokman Lestari, Widya Mustafa Al-Ahmad, Basma Ezzat Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah
author_facet	Nordin, Nur Sazwi Md. Isa, Muhammad Lokman Lestari, Widya Mustafa Al-Ahmad, Basma Ezzat Arief Ichwan, Solachuddin Jauhari Darnis, Deny Susanti Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah
author_sort	Nordin, Nur Sazwi
title	Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
title_short	Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
title_full	Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
title_fullStr	Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
title_full_unstemmed	Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)
title_sort	study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (shed)
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/59676/ http://irep.iium.edu.my/59676/ http://irep.iium.edu.my/59676/1/Nursazwi%20et%20al-2017%20IIDC-IMJM%20Vol%2016%20Supp%202.pdf
first_indexed	2023-09-18T21:24:33Z
last_indexed	2023-09-18T21:24:33Z
_version_	1777412103813988352

Study on flaxseed crude extract on stem cells from human exfoliated deciduous teeth (SHED)

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