Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia

Introduction: The dopamine hypothesis has earlier dominated the theories for the development of schizophrenia based on the early pharmacologic evidence. The antipsychotic drugs, among others, is thought to interfere with the function of the dopamine D2 receptor (DRD2) resulting in clinical improveme...

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Main Authors:	Abd. Rahim, Nour El Huda, Rahim, Mohd Nabil Fikri, Ku Zaifah, Norsidah, Mohd Noor, Hanisah, Abdullah, Kartini, A. Talib, Norlelawati
Format:	Conference or Workshop Item
Language:	English
Published:	Kulliyyah of Medicine, International Islamic University Malaysia 2017
Subjects:	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Online Access:	http://irep.iium.edu.my/59600/ http://irep.iium.edu.my/59600/ http://irep.iium.edu.my/59600/1/59600_EPIGENETIC%20CHANGES.pdf

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spelling	iium-596002017-11-27T10:18:51Z http://irep.iium.edu.my/59600/ Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia Abd. Rahim, Nour El Huda Rahim, Mohd Nabil Fikri Ku Zaifah, Norsidah Mohd Noor, Hanisah Abdullah, Kartini A. Talib, Norlelawati RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Introduction: The dopamine hypothesis has earlier dominated the theories for the development of schizophrenia based on the early pharmacologic evidence. The antipsychotic drugs, among others, is thought to interfere with the function of the dopamine D2 receptor (DRD2) resulting in clinical improvement. Accumulating evidence suggest the role of epigenetic mechanisms in the pathophysiology of schizophrenia. Despite this, specific evidence linking the DRD2 DNA methylation with schizophrenia is insufficient mainly due to the poor accessibility and limited brain samples. Of late, new data has suggested the global impact of DNA methylation in the development of schizophrenia, thus methylation in the peripheral blood could infer changes in the brain. Therefore, the aim of the study is to assess the DRD2 DNA methylation in the peripheral blood of schizophrenia. Materials and method: The case control study consisted of 138 schizophrenia patients from the Psychiatry Clinic, Hospital Kuantan Ampuan Afzan, Kuantan Pahang and 132 healthy controls from Kuantan district. The genomic DNA from the peripheral blood was bisulfite converted. The DRD2 DNA methylation level was quantitatively measured by using the MethyLight Taqman® assay and normalized with the ALU reference control to give the percentage methylation ratio. The demographic data were calculated using descriptive statistics while parametric variables were compared using independent samples t-test or analysis of covariance. Results: There was a significant hypomethylation of DRD2 in schizophrenia as compared to the control group (p=0.001). The hypomethylation was also significant in males (p=0.007) and females (p=0.036) patients. Conclusion: This study strongly support the possible role of DNA methylation of DRD2 gene could contribute to the pathogenesis of schizophrenia. Kulliyyah of Medicine, International Islamic University Malaysia 2017-08-10 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/59600/1/59600_EPIGENETIC%20CHANGES.pdf Abd. Rahim, Nour El Huda and Rahim, Mohd Nabil Fikri and Ku Zaifah, Norsidah and Mohd Noor, Hanisah and Abdullah, Kartini and A. Talib, Norlelawati (2017) Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia. In: Medical Research Symposium 2017, 10th August 2017, Kuantan, Pahang Darul Makmur, Malaysia. http://iiumedic.net/imjm/v1/about-imjm/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
spellingShingle	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Abd. Rahim, Nour El Huda Rahim, Mohd Nabil Fikri Ku Zaifah, Norsidah Mohd Noor, Hanisah Abdullah, Kartini A. Talib, Norlelawati Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
description	Introduction: The dopamine hypothesis has earlier dominated the theories for the development of schizophrenia based on the early pharmacologic evidence. The antipsychotic drugs, among others, is thought to interfere with the function of the dopamine D2 receptor (DRD2) resulting in clinical improvement. Accumulating evidence suggest the role of epigenetic mechanisms in the pathophysiology of schizophrenia. Despite this, specific evidence linking the DRD2 DNA methylation with schizophrenia is insufficient mainly due to the poor accessibility and limited brain samples. Of late, new data has suggested the global impact of DNA methylation in the development of schizophrenia, thus methylation in the peripheral blood could infer changes in the brain. Therefore, the aim of the study is to assess the DRD2 DNA methylation in the peripheral blood of schizophrenia. Materials and method: The case control study consisted of 138 schizophrenia patients from the Psychiatry Clinic, Hospital Kuantan Ampuan Afzan, Kuantan Pahang and 132 healthy controls from Kuantan district. The genomic DNA from the peripheral blood was bisulfite converted. The DRD2 DNA methylation level was quantitatively measured by using the MethyLight Taqman® assay and normalized with the ALU reference control to give the percentage methylation ratio. The demographic data were calculated using descriptive statistics while parametric variables were compared using independent samples t-test or analysis of covariance. Results: There was a significant hypomethylation of DRD2 in schizophrenia as compared to the control group (p=0.001). The hypomethylation was also significant in males (p=0.007) and females (p=0.036) patients. Conclusion: This study strongly support the possible role of DNA methylation of DRD2 gene could contribute to the pathogenesis of schizophrenia.
format	Conference or Workshop Item
author	Abd. Rahim, Nour El Huda Rahim, Mohd Nabil Fikri Ku Zaifah, Norsidah Mohd Noor, Hanisah Abdullah, Kartini A. Talib, Norlelawati
author_facet	Abd. Rahim, Nour El Huda Rahim, Mohd Nabil Fikri Ku Zaifah, Norsidah Mohd Noor, Hanisah Abdullah, Kartini A. Talib, Norlelawati
author_sort	Abd. Rahim, Nour El Huda
title	Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
title_short	Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
title_full	Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
title_fullStr	Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
title_full_unstemmed	Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia
title_sort	epigenetic changes of drd2 gene in pathogenesis of schizophrenia
publisher	Kulliyyah of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/59600/ http://irep.iium.edu.my/59600/ http://irep.iium.edu.my/59600/1/59600_EPIGENETIC%20CHANGES.pdf
first_indexed	2023-09-18T21:24:27Z
last_indexed	2023-09-18T21:24:27Z
_version_	1777412097782579200

Epigenetic changes of DRD2 gene in pathogenesis of schizophrenia

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