Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake

Lipase possessing thermal stability and organic solvent tolerant are believed to distinctively enhance lipase-catalyzed reactions of industrial importance. This study sought to explore the potential of palm kernel cake (PKC) to act as an oil and lipid rich environment for fungal growth, as well as a...

Full description

Bibliographic Details
Main Authors: Riadi, Fatimah Azizah, Alam, Md. Zahangir, Salleh, Md. Noor, Mohd. Salleh, Hamzah
Format: Article
Language:English
English
Published: Malaysian Society for Molecular Biology and Biotechnology 2017
Subjects:
Online Access:http://irep.iium.edu.my/59596/
http://irep.iium.edu.my/59596/
http://irep.iium.edu.my/59596/1/59596_Thermostable%20and%20Organic%20Solvent%20Tolerant%20Lipase%20Producing.pdf
http://irep.iium.edu.my/59596/7/59596_Thermostable%20and%20organic%20solvent%20tolerant%20lipase_scopus.pdf
id iium-59596
recordtype eprints
spelling iium-595962019-08-21T08:50:09Z http://irep.iium.edu.my/59596/ Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake Riadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md. Noor Mohd. Salleh, Hamzah TP248.13 Biotechnology Lipase possessing thermal stability and organic solvent tolerant are believed to distinctively enhance lipase-catalyzed reactions of industrial importance. This study sought to explore the potential of palm kernel cake (PKC) to act as an oil and lipid rich environment for fungal growth, as well as a substrate to produce thermostable and organic solvent tolerant lipase through solid-state bioconversion. Four thermal and solvent tolerant fungi have successfully isolated with lipase producing capabilities. The isolates were screened based on the clearing zone methods on phenol red as well as on tween 80 agar media and quantified using solid-state bioconversion. Among the four isolates, PKC12B2 displayed highest clearance zone on the plate and lipase activities with 5.83 U/gram dry PKC. Morphological identification of PKC12B2 showed the characteristic of genus Rhizopus sp. Malaysian Society for Molecular Biology and Biotechnology 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59596/1/59596_Thermostable%20and%20Organic%20Solvent%20Tolerant%20Lipase%20Producing.pdf application/pdf en http://irep.iium.edu.my/59596/7/59596_Thermostable%20and%20organic%20solvent%20tolerant%20lipase_scopus.pdf Riadi, Fatimah Azizah and Alam, Md. Zahangir and Salleh, Md. Noor and Mohd. Salleh, Hamzah (2017) Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake. Asia Pacific Journal of Molecular Biology and Biotechnology, 25 (1). pp. 98-105. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html251/251i.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Riadi, Fatimah Azizah
Alam, Md. Zahangir
Salleh, Md. Noor
Mohd. Salleh, Hamzah
Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
description Lipase possessing thermal stability and organic solvent tolerant are believed to distinctively enhance lipase-catalyzed reactions of industrial importance. This study sought to explore the potential of palm kernel cake (PKC) to act as an oil and lipid rich environment for fungal growth, as well as a substrate to produce thermostable and organic solvent tolerant lipase through solid-state bioconversion. Four thermal and solvent tolerant fungi have successfully isolated with lipase producing capabilities. The isolates were screened based on the clearing zone methods on phenol red as well as on tween 80 agar media and quantified using solid-state bioconversion. Among the four isolates, PKC12B2 displayed highest clearance zone on the plate and lipase activities with 5.83 U/gram dry PKC. Morphological identification of PKC12B2 showed the characteristic of genus Rhizopus sp.
format Article
author Riadi, Fatimah Azizah
Alam, Md. Zahangir
Salleh, Md. Noor
Mohd. Salleh, Hamzah
author_facet Riadi, Fatimah Azizah
Alam, Md. Zahangir
Salleh, Md. Noor
Mohd. Salleh, Hamzah
author_sort Riadi, Fatimah Azizah
title Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_short Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_full Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_fullStr Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_full_unstemmed Thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
title_sort thermostable and organic solvent tolerant lipase producing fungi in solid state bioconversion of palm kernel cake
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2017
url http://irep.iium.edu.my/59596/
http://irep.iium.edu.my/59596/
http://irep.iium.edu.my/59596/1/59596_Thermostable%20and%20Organic%20Solvent%20Tolerant%20Lipase%20Producing.pdf
http://irep.iium.edu.my/59596/7/59596_Thermostable%20and%20organic%20solvent%20tolerant%20lipase_scopus.pdf
first_indexed 2023-09-18T21:24:26Z
last_indexed 2023-09-18T21:24:26Z
_version_ 1777412097185939456