The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres

Mycoplasma pulmonis is one of the major causes for arthritis which can be treated with Gentamicin. The fabrication of Gentamicin with PLGA micropsheres by using multiple-emulsion (w/o/w) solvent evaporation method can be used to treat this disorder. The resultant micropsheres were characterized with...

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Main Authors:	Harun Ismail, Ahmad Fahmi, Doolane, Abd Monem, Mohamed, Farahidah
Format:	Conference or Workshop Item
Language:	English
Published:	unknown 2010
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/5953/ http://irep.iium.edu.my/5953/ http://irep.iium.edu.my/5953/2/The_study_of_the_effects_of_different_surfactants.pdf

id	iium-5953
recordtype	eprints
spelling	iium-59532012-02-21T03:51:48Z http://irep.iium.edu.my/5953/ The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres Harun Ismail, Ahmad Fahmi Doolane, Abd Monem Mohamed, Farahidah RS Pharmacy and materia medica Mycoplasma pulmonis is one of the major causes for arthritis which can be treated with Gentamicin. The fabrication of Gentamicin with PLGA micropsheres by using multiple-emulsion (w/o/w) solvent evaporation method can be used to treat this disorder. The resultant micropsheres were characterized with scanning electron microscopy (SEM) for external morphology and the size analysis was done by using laser diffraction. The encapsulation efficiency was carried out using ninhydrin assay method to quantify the Gentamicin concentration by UV spectrophotometer at 400 nm. Our aim was to investigate the effects of using different concentration of PVA, different molecular weight of PLGA and different types of surfactants on several parameters which included external morphology of the micropsheres, the size distribution, the encapsulation efficiency and also in-vitro release profile of Gentamicin. Our result showed that the concentration of PVA was inversely proportional to the size of micropsheres as 1% PVA yielded mean size distribution of micropsheres around 13.68 μm (± 0.052 μm) whereas 5% of PVA yielded 3.78 μm (± 0.008 μm). This ongoing research is aimed to transfect Gentamicin-loaded PLGA micropsheres onto Human Osteosarcoma Cell Line (Saos-2). Success of the study will indicate promising use of a novel Gentamicin delivery system for therapy in bone diseases. unknown 2010-11-29 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5953/2/The_study_of_the_effects_of_different_surfactants.pdf Harun Ismail, Ahmad Fahmi and Doolane, Abd Monem and Mohamed, Farahidah (2010) The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres. In: 3rd International Conference on Advancement in Science and Technology (iCAST) 2010, 27-29 November 2010, Vistana Hotel, Kuantan, Pahang. http://www.iium.edu.my/icast/2010/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Harun Ismail, Ahmad Fahmi Doolane, Abd Monem Mohamed, Farahidah The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
description	Mycoplasma pulmonis is one of the major causes for arthritis which can be treated with Gentamicin. The fabrication of Gentamicin with PLGA micropsheres by using multiple-emulsion (w/o/w) solvent evaporation method can be used to treat this disorder. The resultant micropsheres were characterized with scanning electron microscopy (SEM) for external morphology and the size analysis was done by using laser diffraction. The encapsulation efficiency was carried out using ninhydrin assay method to quantify the Gentamicin concentration by UV spectrophotometer at 400 nm. Our aim was to investigate the effects of using different concentration of PVA, different molecular weight of PLGA and different types of surfactants on several parameters which included external morphology of the micropsheres, the size distribution, the encapsulation efficiency and also in-vitro release profile of Gentamicin. Our result showed that the concentration of PVA was inversely proportional to the size of micropsheres as 1% PVA yielded mean size distribution of micropsheres around 13.68 μm (± 0.052 μm) whereas 5% of PVA yielded 3.78 μm (± 0.008 μm). This ongoing research is aimed to transfect Gentamicin-loaded PLGA micropsheres onto Human Osteosarcoma Cell Line (Saos-2). Success of the study will indicate promising use of a novel Gentamicin delivery system for therapy in bone diseases.
format	Conference or Workshop Item
author	Harun Ismail, Ahmad Fahmi Doolane, Abd Monem Mohamed, Farahidah
author_facet	Harun Ismail, Ahmad Fahmi Doolane, Abd Monem Mohamed, Farahidah
author_sort	Harun Ismail, Ahmad Fahmi
title	The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
title_short	The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
title_full	The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
title_fullStr	The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
title_full_unstemmed	The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres
title_sort	study of the effects of different surfactants, concentration of pva and molecular weight of plga on the development of gentamicin-loaded plga microspheres
publisher	unknown
publishDate	2010
url	http://irep.iium.edu.my/5953/ http://irep.iium.edu.my/5953/ http://irep.iium.edu.my/5953/2/The_study_of_the_effects_of_different_surfactants.pdf
first_indexed	2023-09-18T20:14:46Z
last_indexed	2023-09-18T20:14:46Z
_version_	1777407714080587776

The study of the effects of different surfactants, concentration of PVA and molecular weight of PLGA on the development of Gentamicin-loaded PLGA microspheres

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