Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study

Determination of HCV genotype is important for the disease prognosis and prediction of treatment outcomes. Several methods of genotyping are commercially available and others are done in-house. Aims for the study were to elucidate nucleotide sequence of local HCV strains and to establish an in-house...

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Main Author:	Hamzah, Hairul Aini
Format:	Monograph
Language:	English
Published:	2017
Subjects:	QR Microbiology QR355 Virology
Online Access:	http://irep.iium.edu.my/59486/ http://irep.iium.edu.my/59486/1/EDW%20End%20of%20Project%20Report.pdf

id	iium-59486
recordtype	eprints
spelling	iium-594862018-05-17T04:03:13Z http://irep.iium.edu.my/59486/ Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study Hamzah, Hairul Aini QR Microbiology QR355 Virology Determination of HCV genotype is important for the disease prognosis and prediction of treatment outcomes. Several methods of genotyping are commercially available and others are done in-house. Aims for the study were to elucidate nucleotide sequence of local HCV strains and to establish an in-house technique for HCV genotyping using DNA intercalating dye real-time PCR. HCV RNA was extracted and subjected to reverse transcription before amplification using SsoFast EvaGreen mix real-time PCR. Sequence analysis of the 5’UTR revealed two major groups of HCV genotypes (1 and 3). The GC content HCV genotype 1 and 3 were 57.14-58.44% and 55.19-57.14, respectively. HCV RNA was able to be detected with the PCR mix reaction. However, both main genotypes are indiscriminately clustered using high resolution melting analysis and melting temperature analysis. Further modification of the established method could result in better outcomes. 2017-11-16 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/59486/1/EDW%20End%20of%20Project%20Report.pdf Hamzah, Hairul Aini (2017) Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study. Project Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology QR355 Virology
spellingShingle	QR Microbiology QR355 Virology Hamzah, Hairul Aini Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
description	Determination of HCV genotype is important for the disease prognosis and prediction of treatment outcomes. Several methods of genotyping are commercially available and others are done in-house. Aims for the study were to elucidate nucleotide sequence of local HCV strains and to establish an in-house technique for HCV genotyping using DNA intercalating dye real-time PCR. HCV RNA was extracted and subjected to reverse transcription before amplification using SsoFast EvaGreen mix real-time PCR. Sequence analysis of the 5’UTR revealed two major groups of HCV genotypes (1 and 3). The GC content HCV genotype 1 and 3 were 57.14-58.44% and 55.19-57.14, respectively. HCV RNA was able to be detected with the PCR mix reaction. However, both main genotypes are indiscriminately clustered using high resolution melting analysis and melting temperature analysis. Further modification of the established method could result in better outcomes.
format	Monograph
author	Hamzah, Hairul Aini
author_facet	Hamzah, Hairul Aini
author_sort	Hamzah, Hairul Aini
title	Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
title_short	Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
title_full	Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
title_fullStr	Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
title_full_unstemmed	Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study
title_sort	development of hcv genotyping protocol based on high resolution melting analysis real-time pcr: a pilot study
publishDate	2017
url	http://irep.iium.edu.my/59486/ http://irep.iium.edu.my/59486/1/EDW%20End%20of%20Project%20Report.pdf
first_indexed	2023-09-18T21:24:16Z
last_indexed	2023-09-18T21:24:16Z
_version_	1777412086938206208

Development of HCV genotyping protocol based on high resolution melting analysis real-time PCR: a pilot study

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