Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition

Natural products are a vast source of potential compounds that can be developed as anti-obesity agent. One of the mechanisms of anti-obesity agents is inhibition of pancreatic lipase. Assay of 24 crude extracts for their in vitro activity against porcine pancreatic lipase (PPL) detected four extract...

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Main Authors:	Alias, Norsyuhada, Leow, Thean Chor, Mohamad Ali, Mohd. Shukuri, Ahmad Tajudin, Asilah, Salleh, Abu Bakar, Raja Abd Rahman, Raja Noor Zaliha
Format:	Article
Language:	English
Published:	MedCrave 2017
Subjects:	RM Therapeutics. Pharmacology
Online Access:	http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/1/AOWMC-06-00163.pdf

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recordtype	eprints
spelling	iium-594552018-01-11T05:29:02Z http://irep.iium.edu.my/59455/ Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition Alias, Norsyuhada Leow, Thean Chor Mohamad Ali, Mohd. Shukuri Ahmad Tajudin, Asilah Salleh, Abu Bakar Raja Abd Rahman, Raja Noor Zaliha RM Therapeutics. Pharmacology Natural products are a vast source of potential compounds that can be developed as anti-obesity agent. One of the mechanisms of anti-obesity agents is inhibition of pancreatic lipase. Assay of 24 crude extracts for their in vitro activity against porcine pancreatic lipase (PPL) detected four extracts demonstrating high (>70%) inhibition, seven extracts had medium (30-70%) inhibition and the remaining 13 extracts exhibited low (<30%) inhibition when incubated with PPL at a concentration of 500 μg/ml for 10 min at 37°C. Phyllanthus niruri extract displayed the most potent PPL inhibitor, followed by Orthosiphon stamineus, Murraya paniculata and Averrhoa bilimbi with the IC50 value of 27.7<34.7< 41.5<55.2 μg/ml, respectively. P. niruri & O. stamineus (the best two extracts) showed noncompetitive and uncompetitive inhibition, respectively. P. niruri & O. stamineus displayed total phenolic content of 431.0 ± 0.01 and 103.0 ± 0.01 mg GAE/g dry extract, while total flavonoid content of 14.8 ± 0.07 and 21.6 ± 0.03 mg QE/g dry extract, respectively. Both P. niruri & O. stamineus extracts showed high antioxidant activity, with EC50 values of 8.4 and 26.3 μg/ml, respectively. The results suggest that P. niruri & O. stamineus may be beneficial for obesity treatment via pancreatic lipase inhibition action. MedCrave 2017-04-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59455/1/AOWMC-06-00163.pdf Alias, Norsyuhada and Leow, Thean Chor and Mohamad Ali, Mohd. Shukuri and Ahmad Tajudin, Asilah and Salleh, Abu Bakar and Raja Abd Rahman, Raja Noor Zaliha (2017) Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition. Advances in Obesity, Weight Management & Control, 6 (4). pp. 1-11. ISSN 2378-3168 http://medcraveonline.com/AOWMC/AOWMC-06-00163.php 10.15406/aowmc.2017.06.00163
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RM Therapeutics. Pharmacology
spellingShingle	RM Therapeutics. Pharmacology Alias, Norsyuhada Leow, Thean Chor Mohamad Ali, Mohd. Shukuri Ahmad Tajudin, Asilah Salleh, Abu Bakar Raja Abd Rahman, Raja Noor Zaliha Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
description	Natural products are a vast source of potential compounds that can be developed as anti-obesity agent. One of the mechanisms of anti-obesity agents is inhibition of pancreatic lipase. Assay of 24 crude extracts for their in vitro activity against porcine pancreatic lipase (PPL) detected four extracts demonstrating high (>70%) inhibition, seven extracts had medium (30-70%) inhibition and the remaining 13 extracts exhibited low (<30%) inhibition when incubated with PPL at a concentration of 500 μg/ml for 10 min at 37°C. Phyllanthus niruri extract displayed the most potent PPL inhibitor, followed by Orthosiphon stamineus, Murraya paniculata and Averrhoa bilimbi with the IC50 value of 27.7<34.7< 41.5<55.2 μg/ml, respectively. P. niruri & O. stamineus (the best two extracts) showed noncompetitive and uncompetitive inhibition, respectively. P. niruri & O. stamineus displayed total phenolic content of 431.0 ± 0.01 and 103.0 ± 0.01 mg GAE/g dry extract, while total flavonoid content of 14.8 ± 0.07 and 21.6 ± 0.03 mg QE/g dry extract, respectively. Both P. niruri & O. stamineus extracts showed high antioxidant activity, with EC50 values of 8.4 and 26.3 μg/ml, respectively. The results suggest that P. niruri & O. stamineus may be beneficial for obesity treatment via pancreatic lipase inhibition action.
format	Article
author	Alias, Norsyuhada Leow, Thean Chor Mohamad Ali, Mohd. Shukuri Ahmad Tajudin, Asilah Salleh, Abu Bakar Raja Abd Rahman, Raja Noor Zaliha
author_facet	Alias, Norsyuhada Leow, Thean Chor Mohamad Ali, Mohd. Shukuri Ahmad Tajudin, Asilah Salleh, Abu Bakar Raja Abd Rahman, Raja Noor Zaliha
author_sort	Alias, Norsyuhada
title	Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
title_short	Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
title_full	Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
title_fullStr	Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
title_full_unstemmed	Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
title_sort	anti-obesity potential of selected tropical plants via pancreatic lipase inhibition
publisher	MedCrave
publishDate	2017
url	http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/ http://irep.iium.edu.my/59455/1/AOWMC-06-00163.pdf
first_indexed	2023-09-18T21:24:14Z
last_indexed	2023-09-18T21:24:14Z
_version_	1777412084465664000

Anti-obesity potential of selected tropical plants via pancreatic lipase inhibition

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