Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake

This study enhanced the production of thermostable organic solvent-tolerant (TS-OST) lipase by locally isolated thermotolerant Rhizopus sp. strain using solid-state fermentation (SSF) of palm kernel cake (PKC). The optimum conditions were achieved using a series of statistical approaches. The cultiv...

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Main Authors:	Riyadi, Fatimah Azizah, Alam, Md. Zahangir, Salleh, Md. Noor, Mohd Salleh, Hamzah
Format:	Article
Language:	English English
Published:	Springer Berlin Heidelberg 2017
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/1/Paper%20Fatimah%20optimization.pdf http://irep.iium.edu.my/59426/7/59426-Optimization%20of%20thermostable%20organic%20solvent-tolerant%20lipase_SCOPUS.pdf

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recordtype	eprints
spelling	iium-594262018-03-09T07:20:11Z http://irep.iium.edu.my/59426/ Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md. Noor Mohd Salleh, Hamzah TP248.13 Biotechnology This study enhanced the production of thermostable organic solvent-tolerant (TS-OST) lipase by locally isolated thermotolerant Rhizopus sp. strain using solid-state fermentation (SSF) of palm kernel cake (PKC). The optimum conditions were achieved using a series of statistical approaches. The cultivation parameters, which include fermentation time, moisture content, temperature, pH, inoculum size, various carbon and nitrogen sources, as well as other supplements, were initially screened by the definitive screening design, and one-factor-at-a-time using PKC as the basal medium. Three significant factors (olive oil concentration, pH, and inoculum size) were further optimized using face-centred central composite design. The results indicated a successful and significant improvement of lipase activity by almost two-fold compared to the initial screening production. The findings showed that the optimal conditions were 2% (v/w) inoculum size, 2% (v/w) olive oil, 0.6% (w/w) peptone, 2% (v/w) ethanol, 70% moisture content at initial pH 10.0 and 45 °C within 72 h of fermentation. Process optimization resulted in maximum lipase activity of 58.63 U/gram dry solids (gds). The analysis of variance showed that the statistical model was significant (p value <0.0001) and reliable with a high value of R2 (0.98) and adjusted R2 (0.96). This indicates a better correlation between the actual and predicted responses of lipase production. By considering this study, the low-cost PKC through SSF appears to be promising in the utilization of agro-industrial waste for TS-OST lipase production. This is because satisfactory enzyme activity could be attained that promises industrial applications Springer Berlin Heidelberg 2017-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59426/1/Paper%20Fatimah%20optimization.pdf application/pdf en http://irep.iium.edu.my/59426/7/59426-Optimization%20of%20thermostable%20organic%20solvent-tolerant%20lipase_SCOPUS.pdf Riyadi, Fatimah Azizah and Alam, Md. Zahangir and Salleh, Md. Noor and Mohd Salleh, Hamzah (2017) Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake. 3 Biotech, 7 (5). pp. 1-11. ISSN 2190-572X E-ISSN 2190-5738 https://link.springer.com/article/10.1007/s13205-017-0932-1 10.1007/s13205-017-0932-1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	TP248.13 Biotechnology
spellingShingle	TP248.13 Biotechnology Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md. Noor Mohd Salleh, Hamzah Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
description	This study enhanced the production of thermostable organic solvent-tolerant (TS-OST) lipase by locally isolated thermotolerant Rhizopus sp. strain using solid-state fermentation (SSF) of palm kernel cake (PKC). The optimum conditions were achieved using a series of statistical approaches. The cultivation parameters, which include fermentation time, moisture content, temperature, pH, inoculum size, various carbon and nitrogen sources, as well as other supplements, were initially screened by the definitive screening design, and one-factor-at-a-time using PKC as the basal medium. Three significant factors (olive oil concentration, pH, and inoculum size) were further optimized using face-centred central composite design. The results indicated a successful and significant improvement of lipase activity by almost two-fold compared to the initial screening production. The findings showed that the optimal conditions were 2% (v/w) inoculum size, 2% (v/w) olive oil, 0.6% (w/w) peptone, 2% (v/w) ethanol, 70% moisture content at initial pH 10.0 and 45 °C within 72 h of fermentation. Process optimization resulted in maximum lipase activity of 58.63 U/gram dry solids (gds). The analysis of variance showed that the statistical model was significant (p value <0.0001) and reliable with a high value of R2 (0.98) and adjusted R2 (0.96). This indicates a better correlation between the actual and predicted responses of lipase production. By considering this study, the low-cost PKC through SSF appears to be promising in the utilization of agro-industrial waste for TS-OST lipase production. This is because satisfactory enzyme activity could be attained that promises industrial applications
format	Article
author	Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md. Noor Mohd Salleh, Hamzah
author_facet	Riyadi, Fatimah Azizah Alam, Md. Zahangir Salleh, Md. Noor Mohd Salleh, Hamzah
author_sort	Riyadi, Fatimah Azizah
title	Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
title_short	Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
title_full	Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
title_fullStr	Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
title_full_unstemmed	Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake
title_sort	optimization of thermostable organic solvent-tolerant lipase production by thermotolerant rhizopus sp. using solid-state fermentation of palm kernel cake
publisher	Springer Berlin Heidelberg
publishDate	2017
url	http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/ http://irep.iium.edu.my/59426/1/Paper%20Fatimah%20optimization.pdf http://irep.iium.edu.my/59426/7/59426-Optimization%20of%20thermostable%20organic%20solvent-tolerant%20lipase_SCOPUS.pdf
first_indexed	2023-09-18T21:24:12Z
last_indexed	2023-09-18T21:24:12Z
_version_	1777412082721882112

Optimization of thermostable organic solvent-tolerant lipase production by thermotolerant Rhizopus sp. using solid-state fermentation of palm kernel cake

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