Autologous serum supplement favours in vitro regenerative paracrine factors synthesis

Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The...

Full description

Bibliographic Details
Main Authors:	Haque, Nazmul, Abu Kasim, Noor Hayaty, Abu Kassim, Noor Lide, Rahman, Mohammad Tariqur
Format:	Article
Language:	English English English
Published:	Wiley-Blackwell 2017
Subjects:	RC Internal medicine RE Ophthalmology RK Dentistry
Online Access:	http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf

id	iium-59216
recordtype	eprints
spelling	iium-592162018-06-21T00:50:45Z http://irep.iium.edu.my/59216/ Autologous serum supplement favours in vitro regenerative paracrine factors synthesis Haque, Nazmul Abu Kasim, Noor Hayaty Abu Kassim, Noor Lide Rahman, Mohammad Tariqur RC Internal medicine RE Ophthalmology RK Dentistry Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated. Paracrine factors present in the secretomes (n = 6) were analysed using ProcartaPlex Human Cytokine panel (17 plex). Ingenuity Pathway Analysis (IPA) was performed to predict activation or inhibition of biological functions related to tissue regeneration. Results: The viability of PBMC that were cultured with FBS supplement was significantly reduced at 96 h compared to those at 0 and 24 h (P <.05). While the reduction of the viability of PBMC that were cultured with AuHS supplement was not significantly different compared to those at 0 and 24 h. The FBS secretomes prepared at 24 h was found to contain significantly higher amount of EGF (P <.05) compared to that in AuHS or BM secretome. The AuHS secretomes contained significantly higher amount of HGF at 24 (P <.05) and 96 h (P <.01), and VEGF-A at 24 h (P <.05) compared to those in the FBS secretomes. SDF-1 was not detected in the FBS secretomes prepared at either 24 or 96 hours. Double immunocytochemical staining revealed a marked increase in co-localization of SDF-1 and its receptor in PBMC that were cultured with AuHS supplement compared to that cultured with FBS supplement. Conclusion: In secretome preparation, AuHS supplement favours synthesis of paracrine factors that are needed for regenerative therapy. Wiley-Blackwell 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf application/pdf en http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf application/pdf en http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf Haque, Nazmul and Abu Kasim, Noor Hayaty and Abu Kassim, Noor Lide and Rahman, Mohammad Tariqur (2017) Autologous serum supplement favours in vitro regenerative paracrine factors synthesis. Cell Proliferation, 50 (4). e12354-1. ISSN 0960-7722 E-ISSN 1365-2184 http://onlinelibrary.wiley.com/doi/10.1111/cpr.12354/abstract 10.1111/cpr.12354
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RC Internal medicine RE Ophthalmology RK Dentistry
spellingShingle	RC Internal medicine RE Ophthalmology RK Dentistry Haque, Nazmul Abu Kasim, Noor Hayaty Abu Kassim, Noor Lide Rahman, Mohammad Tariqur Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
description	Objectives: Foetal bovine serum (FBS) is often the serum supplement of choice for in vitro human cell culture. This study compares the effect of FBS and autologous human serum (AuHS) supplement in human peripheral blood mononuclear cell (PBMC) culture to prepare secretome. Materials and methods: The PBMC (n = 7) were cultured either in RPMI-1640 containing L-glutamine and 50 units/ml Penicillin-Streptomycin (BM) or in BM with either AuHS or FBS. Viability, proliferation and differentiation of PBMC were evaluated. Paracrine factors present in the secretomes (n = 6) were analysed using ProcartaPlex Human Cytokine panel (17 plex). Ingenuity Pathway Analysis (IPA) was performed to predict activation or inhibition of biological functions related to tissue regeneration. Results: The viability of PBMC that were cultured with FBS supplement was significantly reduced at 96 h compared to those at 0 and 24 h (P <.05). While the reduction of the viability of PBMC that were cultured with AuHS supplement was not significantly different compared to those at 0 and 24 h. The FBS secretomes prepared at 24 h was found to contain significantly higher amount of EGF (P <.05) compared to that in AuHS or BM secretome. The AuHS secretomes contained significantly higher amount of HGF at 24 (P <.05) and 96 h (P <.01), and VEGF-A at 24 h (P <.05) compared to those in the FBS secretomes. SDF-1 was not detected in the FBS secretomes prepared at either 24 or 96 hours. Double immunocytochemical staining revealed a marked increase in co-localization of SDF-1 and its receptor in PBMC that were cultured with AuHS supplement compared to that cultured with FBS supplement. Conclusion: In secretome preparation, AuHS supplement favours synthesis of paracrine factors that are needed for regenerative therapy.
format	Article
author	Haque, Nazmul Abu Kasim, Noor Hayaty Abu Kassim, Noor Lide Rahman, Mohammad Tariqur
author_facet	Haque, Nazmul Abu Kasim, Noor Hayaty Abu Kassim, Noor Lide Rahman, Mohammad Tariqur
author_sort	Haque, Nazmul
title	Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_short	Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_full	Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_fullStr	Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_full_unstemmed	Autologous serum supplement favours in vitro regenerative paracrine factors synthesis
title_sort	autologous serum supplement favours in vitro regenerative paracrine factors synthesis
publisher	Wiley-Blackwell
publishDate	2017
url	http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/ http://irep.iium.edu.my/59216/1/59216_Autologous%20serum%20supplement%20favours_article.pdf http://irep.iium.edu.my/59216/2/59216_Autologous%20serum%20supplement%20favours_scopus.pdf http://irep.iium.edu.my/59216/13/59216_Autologous%20serum%20supplement%20favours_ABSTRACT.pdf
first_indexed	2023-09-18T21:23:51Z
last_indexed	2023-09-18T21:23:51Z
_version_	1777412060720660480

Autologous serum supplement favours in vitro regenerative paracrine factors synthesis

Similar Items