The development of in vitro human fibroblast feeder line

Human embryonic stem cells (hESCs) need feeder cells for their maintenance in an undifferentiated state. In conventional culture systems, mouse embryonic fibroblasts (MEFs) serve as feeder cells to maintain hESCs. These cells are pluripotent, immortal, and they retain their developmental potential a...

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Main Authors:	Mat Yusof, Afzan, Md Isa, Muhammad Lokman
Format:	Monograph
Language:	English
Published:	2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/59071/ http://irep.iium.edu.my/59071/1/End%20Report%20RIGS-2017.pdf

id	iium-59071
recordtype	eprints
spelling	iium-590712018-05-17T04:15:10Z http://irep.iium.edu.my/59071/ The development of in vitro human fibroblast feeder line Mat Yusof, Afzan Md Isa, Muhammad Lokman Q Science (General) Human embryonic stem cells (hESCs) need feeder cells for their maintenance in an undifferentiated state. In conventional culture systems, mouse embryonic fibroblasts (MEFs) serve as feeder cells to maintain hESCs. These cells are pluripotent, immortal, and they retain their developmental potential after prolonged culture and maintain normal karyotypes after continuous culture. Given the outstanding potential of human ES cell lines, their availability offers a unique and novel research tool with prospective widespread clinical applications. Human ES cells may be utilized in the future in various research areas, such as human developmental biology, teratology, and cell-based therapies. Contrary to human ES, mouse ES cells can be grown directly on gelatin-coated plates with the addition of leukemia inhibitory factor. Handling the simultaneous growth of both ES cells and MEF requires meticulous care and may prove to be rather expensive. In addition, the dual growth of these cells exposes the human ES cells to mouse retroviruses, which may prevent their future use in cell-based therapy, thus limits the potential of hESCs in cell replacement therapy. Consequently, the use of human feeder cells would be an important step forward in this in vitro technology. Therefore, objectives of this research were to prepare feeder culture by using human fibroblast, to stabilize human fibroblast feeder culture from being contaminated and to observe the changes that occur on human fibroblast feeder culture after undergoing 5-10 passages. The biopsy tissue was obtained from IVF Centre IIUM of normal patient undergo IVF treatment with consent. It was found there was no contamination in term of pathogens after human fibroblast feeder culture is being stabilized. It is also found that there were no significant changes on human fibroblast feeder culture after undergoing 5-10 passages. 2017-11-01 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/59071/1/End%20Report%20RIGS-2017.pdf Mat Yusof, Afzan and Md Isa, Muhammad Lokman (2017) The development of in vitro human fibroblast feeder line. Research Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Mat Yusof, Afzan Md Isa, Muhammad Lokman The development of in vitro human fibroblast feeder line
description	Human embryonic stem cells (hESCs) need feeder cells for their maintenance in an undifferentiated state. In conventional culture systems, mouse embryonic fibroblasts (MEFs) serve as feeder cells to maintain hESCs. These cells are pluripotent, immortal, and they retain their developmental potential after prolonged culture and maintain normal karyotypes after continuous culture. Given the outstanding potential of human ES cell lines, their availability offers a unique and novel research tool with prospective widespread clinical applications. Human ES cells may be utilized in the future in various research areas, such as human developmental biology, teratology, and cell-based therapies. Contrary to human ES, mouse ES cells can be grown directly on gelatin-coated plates with the addition of leukemia inhibitory factor. Handling the simultaneous growth of both ES cells and MEF requires meticulous care and may prove to be rather expensive. In addition, the dual growth of these cells exposes the human ES cells to mouse retroviruses, which may prevent their future use in cell-based therapy, thus limits the potential of hESCs in cell replacement therapy. Consequently, the use of human feeder cells would be an important step forward in this in vitro technology. Therefore, objectives of this research were to prepare feeder culture by using human fibroblast, to stabilize human fibroblast feeder culture from being contaminated and to observe the changes that occur on human fibroblast feeder culture after undergoing 5-10 passages. The biopsy tissue was obtained from IVF Centre IIUM of normal patient undergo IVF treatment with consent. It was found there was no contamination in term of pathogens after human fibroblast feeder culture is being stabilized. It is also found that there were no significant changes on human fibroblast feeder culture after undergoing 5-10 passages.
format	Monograph
author	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_facet	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_sort	Mat Yusof, Afzan
title	The development of in vitro human fibroblast feeder line
title_short	The development of in vitro human fibroblast feeder line
title_full	The development of in vitro human fibroblast feeder line
title_fullStr	The development of in vitro human fibroblast feeder line
title_full_unstemmed	The development of in vitro human fibroblast feeder line
title_sort	development of in vitro human fibroblast feeder line
publishDate	2017
url	http://irep.iium.edu.my/59071/ http://irep.iium.edu.my/59071/1/End%20Report%20RIGS-2017.pdf
first_indexed	2023-09-18T21:23:38Z
last_indexed	2023-09-18T21:23:38Z
_version_	1777412046407598080

The development of in vitro human fibroblast feeder line

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