Palm oil mill effluent metagenome for cellulose-degrading enzymes

Abstract: Functional metagenomic approach incorporating metagenomic DNA library construction and high-throughput screening has proven to be a powerful tool for identifying novel biocatalysts (Wouters et al. 2014). Culture enrichment strategies are additional pre-screening methods employed to provide...

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Main Authors:	Ben Belgacem, Farah Fadwa, Bellag, Qualid Abdelkader, Ahmad, Abdul Aziz, Noorbatcha, Ibrahim Ali, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	Haitham Qaralleh 2017
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/59028/ http://irep.iium.edu.my/59028/ http://irep.iium.edu.my/59028/1/jbaar20172006%20Manuscript%20published.pdf

id	iium-59028
recordtype	eprints
spelling	iium-590282017-11-03T03:04:14Z http://irep.iium.edu.my/59028/ Palm oil mill effluent metagenome for cellulose-degrading enzymes Ben Belgacem, Farah Fadwa Bellag, Qualid Abdelkader Ahmad, Abdul Aziz Noorbatcha, Ibrahim Ali Mohd. Salleh, Hamzah TP Chemical technology TP248.13 Biotechnology Abstract: Functional metagenomic approach incorporating metagenomic DNA library construction and high-throughput screening has proven to be a powerful tool for identifying novel biocatalysts (Wouters et al. 2014). Culture enrichment strategies are additional pre-screening methods employed to provide an attractive means of enhancing the screening hit rate. In this work metagenomic DNA libraries were generated from Malaysian palm oil mill effluent (POME) microorganisms. Three different samples, namely fresh, ambient-cooled and anaerobic POME microorganisms were inoculated in a medium under controlled temperature, light and pH conditions, and in the presence of carboxymethylcellulose (CMC) for short incubation time in order to allow growth of microorganisms with cellulose-degrading capabilities. Quantitative and qualitative metagenomic DNA tests indicate the presence of high number of microbes in anaerobic POME compared to other samples which guided us to use this particular sample in further experiments. Titer test also showed that the number of enriched-library clones is 5 to 7 times higher than non-enriched anaerobic POME. The use of such a combination of enrichment strategy with metagenomics greatly improves the screening process for biocatalysts. Haitham Qaralleh 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59028/1/jbaar20172006%20Manuscript%20published.pdf Ben Belgacem, Farah Fadwa and Bellag, Qualid Abdelkader and Ahmad, Abdul Aziz and Noorbatcha, Ibrahim Ali and Mohd. Salleh, Hamzah (2017) Palm oil mill effluent metagenome for cellulose-degrading enzymes. Journal of basic and applied Research, 3 (3). pp. 101-106. ISSN 2413-7014 http://www.jbaar.com/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Ben Belgacem, Farah Fadwa Bellag, Qualid Abdelkader Ahmad, Abdul Aziz Noorbatcha, Ibrahim Ali Mohd. Salleh, Hamzah Palm oil mill effluent metagenome for cellulose-degrading enzymes
description	Abstract: Functional metagenomic approach incorporating metagenomic DNA library construction and high-throughput screening has proven to be a powerful tool for identifying novel biocatalysts (Wouters et al. 2014). Culture enrichment strategies are additional pre-screening methods employed to provide an attractive means of enhancing the screening hit rate. In this work metagenomic DNA libraries were generated from Malaysian palm oil mill effluent (POME) microorganisms. Three different samples, namely fresh, ambient-cooled and anaerobic POME microorganisms were inoculated in a medium under controlled temperature, light and pH conditions, and in the presence of carboxymethylcellulose (CMC) for short incubation time in order to allow growth of microorganisms with cellulose-degrading capabilities. Quantitative and qualitative metagenomic DNA tests indicate the presence of high number of microbes in anaerobic POME compared to other samples which guided us to use this particular sample in further experiments. Titer test also showed that the number of enriched-library clones is 5 to 7 times higher than non-enriched anaerobic POME. The use of such a combination of enrichment strategy with metagenomics greatly improves the screening process for biocatalysts.
format	Article
author	Ben Belgacem, Farah Fadwa Bellag, Qualid Abdelkader Ahmad, Abdul Aziz Noorbatcha, Ibrahim Ali Mohd. Salleh, Hamzah
author_facet	Ben Belgacem, Farah Fadwa Bellag, Qualid Abdelkader Ahmad, Abdul Aziz Noorbatcha, Ibrahim Ali Mohd. Salleh, Hamzah
author_sort	Ben Belgacem, Farah Fadwa
title	Palm oil mill effluent metagenome for cellulose-degrading enzymes
title_short	Palm oil mill effluent metagenome for cellulose-degrading enzymes
title_full	Palm oil mill effluent metagenome for cellulose-degrading enzymes
title_fullStr	Palm oil mill effluent metagenome for cellulose-degrading enzymes
title_full_unstemmed	Palm oil mill effluent metagenome for cellulose-degrading enzymes
title_sort	palm oil mill effluent metagenome for cellulose-degrading enzymes
publisher	Haitham Qaralleh
publishDate	2017
url	http://irep.iium.edu.my/59028/ http://irep.iium.edu.my/59028/ http://irep.iium.edu.my/59028/1/jbaar20172006%20Manuscript%20published.pdf
first_indexed	2023-09-18T21:23:33Z
last_indexed	2023-09-18T21:23:33Z
_version_	1777412041855729664

Palm oil mill effluent metagenome for cellulose-degrading enzymes

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