Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma

Introduction: Squamous cell carcinoma is reported as one of the most common types of cancer with increasing numbers of occurrence. Luvunga scandens is a plant possessing many bioactivities and general health effects, yet its anti-proliferative effect is under reported and need to be scientifically e...

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Main Authors:	Shaban, Sama, Ichwan, Solachuddin J. A., Bakhtiar, M. Taher
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RB Pathology RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/59000/ http://irep.iium.edu.my/59000/ http://irep.iium.edu.my/59000/1/IIDC2017%20EBOOK%20copy%202.pdf

id	iium-59000
recordtype	eprints
spelling	iium-590002017-11-02T06:37:04Z http://irep.iium.edu.my/59000/ Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma Shaban, Sama Ichwan, Solachuddin J. A. Bakhtiar, M. Taher RB Pathology RS Pharmacy and materia medica Introduction: Squamous cell carcinoma is reported as one of the most common types of cancer with increasing numbers of occurrence. Luvunga scandens is a plant possessing many bioactivities and general health effects, yet its anti-proliferative effect is under reported and need to be scientifically evaluated. Materials and Methods: MTT assay was used to assess the cytotoxicity of the plant against human squamous carcinoma cells in addition to the safety assessment for human dermal fibroblast cell line (HDF). The morphological changes of L. scandens treated squamous carcinoma cells has been confirmed by SEM, the apoptosis of the plant against squamous carcinoma cells has been tested using caspase 3/7 assay, followed by cell cycle analysis done using a flow-cytometer on squamous carcinoma cells treated with the IC50 dose of L. scandens plant. Results: The plant's extract possesses cytotoxic effect against squamous carcinoma cells with IC50 readings; (methanol= 37.5 mg/mL, dichloromethane= 38 mg/mL, hexane= 37.5 mg/mL), and safe on HDF cells. The SEM results demonstrate that L. scandens treated cells showed an overall change in the cell shape, alteration of surface morphology, absence of microvilli and appearance of blebs. Caspase 3/7 assay results show that L. scandens dichloromethane extract produces the highest level of apoptosis against squamous carcinoma cells. For cell cycle analysis, all the L. scandens treated squamous carcinoma cells show high readings in the sub-G1 phase. Conclusion(s): This in vitro study has proved that L. scandens plant exhibit anti-proliferative effects against Squamous carcinoma cells, hence, it can be considered as a new promising potential anti-cancer therapy. 2017-10 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/59000/1/IIDC2017%20EBOOK%20copy%202.pdf Shaban, Sama and Ichwan, Solachuddin J. A. and Bakhtiar, M. Taher (2017) Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma. In: 1st IIUM International Dental Conference 2017 in Commemoration of the Kulliyyah of Dentistry 10th Anniversary (1st IIDC), 6th-8th October 2017, Kuantan, Pahang. (Unpublished) http://iidc.com.my/wp-content/uploads/2017/09/IIDC-eBook.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RB Pathology RS Pharmacy and materia medica
spellingShingle	RB Pathology RS Pharmacy and materia medica Shaban, Sama Ichwan, Solachuddin J. A. Bakhtiar, M. Taher Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
description	Introduction: Squamous cell carcinoma is reported as one of the most common types of cancer with increasing numbers of occurrence. Luvunga scandens is a plant possessing many bioactivities and general health effects, yet its anti-proliferative effect is under reported and need to be scientifically evaluated. Materials and Methods: MTT assay was used to assess the cytotoxicity of the plant against human squamous carcinoma cells in addition to the safety assessment for human dermal fibroblast cell line (HDF). The morphological changes of L. scandens treated squamous carcinoma cells has been confirmed by SEM, the apoptosis of the plant against squamous carcinoma cells has been tested using caspase 3/7 assay, followed by cell cycle analysis done using a flow-cytometer on squamous carcinoma cells treated with the IC50 dose of L. scandens plant. Results: The plant's extract possesses cytotoxic effect against squamous carcinoma cells with IC50 readings; (methanol= 37.5 mg/mL, dichloromethane= 38 mg/mL, hexane= 37.5 mg/mL), and safe on HDF cells. The SEM results demonstrate that L. scandens treated cells showed an overall change in the cell shape, alteration of surface morphology, absence of microvilli and appearance of blebs. Caspase 3/7 assay results show that L. scandens dichloromethane extract produces the highest level of apoptosis against squamous carcinoma cells. For cell cycle analysis, all the L. scandens treated squamous carcinoma cells show high readings in the sub-G1 phase. Conclusion(s): This in vitro study has proved that L. scandens plant exhibit anti-proliferative effects against Squamous carcinoma cells, hence, it can be considered as a new promising potential anti-cancer therapy.
format	Conference or Workshop Item
author	Shaban, Sama Ichwan, Solachuddin J. A. Bakhtiar, M. Taher
author_facet	Shaban, Sama Ichwan, Solachuddin J. A. Bakhtiar, M. Taher
author_sort	Shaban, Sama
title	Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
title_short	Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
title_full	Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
title_fullStr	Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
title_full_unstemmed	Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma
title_sort	anti-cancer activity of luvunga scandens extract against squamous cell carcinoma
publishDate	2017
url	http://irep.iium.edu.my/59000/ http://irep.iium.edu.my/59000/ http://irep.iium.edu.my/59000/1/IIDC2017%20EBOOK%20copy%202.pdf
first_indexed	2023-09-18T21:23:30Z
last_indexed	2023-09-18T21:23:30Z
_version_	1777412038656524288

Anti-cancer activity of Luvunga Scandens extract against squamous cell carcinoma

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