Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca

All carotenoids originate from a single, common precursor, phytoene. The color of carotenoids is determined by desaturation, isomerization, cyclization, hydroxylation and epoxidation of the 40-carbon phytoene. The conjugated double-bond structure and nature of end ring groups confer on the carotenoi...

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Main Authors:	Othman, Rashidi, Abu Bakar, Ainaa Eliah
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/58998/ http://irep.iium.edu.my/58998/ http://irep.iium.edu.my/58998/1/APCC%201.pdf

id	iium-58998
recordtype	eprints
spelling	iium-589982017-11-02T07:37:14Z http://irep.iium.edu.my/58998/ Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca Othman, Rashidi Abu Bakar, Ainaa Eliah GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology All carotenoids originate from a single, common precursor, phytoene. The color of carotenoids is determined by desaturation, isomerization, cyclization, hydroxylation and epoxidation of the 40-carbon phytoene. The conjugated double-bond structure and nature of end ring groups confer on the carotenoids properties such as color and antioxidant activity. Algae may become major sources of carotenoids but the extent of environmental and genetic influences on algae carotenoid biosynthesis are poorly understood. Carotenoid biosynthesis can be influenced by many aspects and is liable to geometric isomerization with the existence of oxygen, light and heat which affect color degradation and oxidation. Therefore, in this study carotenoid biogenesis is investigated in cell culture of Chlorella fusca as a potential model system for rapid initiation, extraction and HPLC analysis of carotenoids by providing stringent control of genetic, developmental and environmental factors. The value of this experimental system for investigating key factors controlling carotenoid accumulation is then tested by assessing the effects of environmental variables, such as drought stress, light intensity and nutrient strength on carotenoid accumulation. Our finding revealed that the conversion of β-carotene to lutein is due to irradiance stress condition, nutrient strength as well as drought stress. As a result, lutein concentration will increase. This reaction will restrict the supply of precursors for ABA biosynthesis and the algae cell culture responds by increasing carotenogenic metabolic flux to compensate for this restriction. In conclusion, selecting the appropriate algae species for the appropriate environmental conditions is not only important for yield production, but also for nutritional value and quality of carotenoid. 2017-10 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/58998/1/APCC%201.pdf Othman, Rashidi and Abu Bakar, Ainaa Eliah (2017) Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca. In: 8th Asian Pacific Phycological Forum, 8th-13th October 2017, Kuala Lumpur. (Unpublished) https://umconference.um.edu.my/APPF2017
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology
spellingShingle	GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology Othman, Rashidi Abu Bakar, Ainaa Eliah Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
description	All carotenoids originate from a single, common precursor, phytoene. The color of carotenoids is determined by desaturation, isomerization, cyclization, hydroxylation and epoxidation of the 40-carbon phytoene. The conjugated double-bond structure and nature of end ring groups confer on the carotenoids properties such as color and antioxidant activity. Algae may become major sources of carotenoids but the extent of environmental and genetic influences on algae carotenoid biosynthesis are poorly understood. Carotenoid biosynthesis can be influenced by many aspects and is liable to geometric isomerization with the existence of oxygen, light and heat which affect color degradation and oxidation. Therefore, in this study carotenoid biogenesis is investigated in cell culture of Chlorella fusca as a potential model system for rapid initiation, extraction and HPLC analysis of carotenoids by providing stringent control of genetic, developmental and environmental factors. The value of this experimental system for investigating key factors controlling carotenoid accumulation is then tested by assessing the effects of environmental variables, such as drought stress, light intensity and nutrient strength on carotenoid accumulation. Our finding revealed that the conversion of β-carotene to lutein is due to irradiance stress condition, nutrient strength as well as drought stress. As a result, lutein concentration will increase. This reaction will restrict the supply of precursors for ABA biosynthesis and the algae cell culture responds by increasing carotenogenic metabolic flux to compensate for this restriction. In conclusion, selecting the appropriate algae species for the appropriate environmental conditions is not only important for yield production, but also for nutritional value and quality of carotenoid.
format	Conference or Workshop Item
author	Othman, Rashidi Abu Bakar, Ainaa Eliah
author_facet	Othman, Rashidi Abu Bakar, Ainaa Eliah
author_sort	Othman, Rashidi
title	Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
title_short	Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
title_full	Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
title_fullStr	Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
title_full_unstemmed	Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
title_sort	manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca
publishDate	2017
url	http://irep.iium.edu.my/58998/ http://irep.iium.edu.my/58998/ http://irep.iium.edu.my/58998/1/APCC%201.pdf
first_indexed	2023-09-18T21:23:30Z
last_indexed	2023-09-18T21:23:30Z
_version_	1777412038367117312

Manipulation of environmental variables towards carotenoid biosynthesis in chlorella fusca

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