Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics

Plant is well known as an excellent source for bioactive compounds. Metabolomics was reported as a potential tool to accelerate plant acitive compounds identification. In this research, FTIR-based metabolomics method was used to identify active compounds with α-glucosidase inhibitory and antioxidant...

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Main Authors:	Juliani, J., Yuliana, Nancy Dewi, Budijanto, Slamet, Wijaya, C. Hanny, Khatib, Alfi
Format:	Article
Language:	English
Published:	Indonesian Food Technologist Association (IAFT) 2016
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/58994/ http://irep.iium.edu.my/58994/ http://irep.iium.edu.my/58994/1/juliani%20et%20al.%20%282016%29.pdf

id	iium-58994
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spelling	iium-589942017-10-31T03:43:24Z http://irep.iium.edu.my/58994/ Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics Juliani, J. Yuliana, Nancy Dewi Budijanto, Slamet Wijaya, C. Hanny Khatib, Alfi Q Science (General) Plant is well known as an excellent source for bioactive compounds. Metabolomics was reported as a potential tool to accelerate plant acitive compounds identification. In this research, FTIR-based metabolomics method was used to identify active compounds with α-glucosidase inhibitory and antioxidant activity in aerial parts of Orthosiphon stamineus (OS) extract and its fractions. Chemical profile of OS methanolic extracts and hexane, chloroform, butanol, and water fractions were analyzed using infrared spectroscopy. OS extracts and fractions showed inhibitory activity against α-glucosidase enzymes with IC50 value 154.07±30.60-465.83±85.34 µg/mL and antioxidant activity with IC50 value 7.41±0.02-19.35±0.09 µg/mL. Butanol fraction was the fraction with the highest α-glucosidase inhibitory activity and moderate antioxidant activity with IC50 value between 154.07±30.60 µg/mL and 10.84±0.54 µg/mL, respectively. The correlation between the biological activity and chemical composition data were analyzed using Orthogonal Projections to Latent Structures (OPLS). Based on the VIP (variable influence on projection), the coefficient value of the respective OPLS models, and IR database of compounds previously identified in OS, it was suggested that methoxy flavonoid (sinensitin and 5,6,7,3’-tetramethoxy-4’-hydroxy-8-C-preny-lflavone), diterpenes (orthosiphols, orthoarisins, neoorthosiphols, staminols, and staminolactones) and triterpenes (ursolic acid, oleanolic acid, betulinic acid, hydroxybetulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsible compounds for the α-glucosidase inhibitory activity. Meanwhile phenolic (rosmarinic acid), methoxy flavonoid (eupatorin, sinensetin, 5-hydroxy-6,7,3’,4’-tetramethoxyflavone, salvigenin, 6-hydroxy-5,7,3’-trimethoxyflavone and 5,6,7,3’-tetramethoxy-4’-hydroxy-8-C-prenylflavone), diterpenes (orthosiphols, orthoarisins, neoortho-siphols, staminols, and staminolactones) and triterpenes (ursolic acid, oleanolic acid, betulinic acid, hydroxybetulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsible compounds for the antioxidant activity. Indonesian Food Technologist Association (IAFT) 2016-01-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58994/1/juliani%20et%20al.%20%282016%29.pdf Juliani, J. and Yuliana, Nancy Dewi and Budijanto, Slamet and Wijaya, C. Hanny and Khatib, Alfi (2016) Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics. Jurnal Teknologi dan Industri Pangan, 27 (1). pp. 17-30. ISSN 1979-7788 E-ISSN 2087-751X http://journal.ipb.ac.id/index.php/jtip/index
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Juliani, J. Yuliana, Nancy Dewi Budijanto, Slamet Wijaya, C. Hanny Khatib, Alfi Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
description	Plant is well known as an excellent source for bioactive compounds. Metabolomics was reported as a potential tool to accelerate plant acitive compounds identification. In this research, FTIR-based metabolomics method was used to identify active compounds with α-glucosidase inhibitory and antioxidant activity in aerial parts of Orthosiphon stamineus (OS) extract and its fractions. Chemical profile of OS methanolic extracts and hexane, chloroform, butanol, and water fractions were analyzed using infrared spectroscopy. OS extracts and fractions showed inhibitory activity against α-glucosidase enzymes with IC50 value 154.07±30.60-465.83±85.34 µg/mL and antioxidant activity with IC50 value 7.41±0.02-19.35±0.09 µg/mL. Butanol fraction was the fraction with the highest α-glucosidase inhibitory activity and moderate antioxidant activity with IC50 value between 154.07±30.60 µg/mL and 10.84±0.54 µg/mL, respectively. The correlation between the biological activity and chemical composition data were analyzed using Orthogonal Projections to Latent Structures (OPLS). Based on the VIP (variable influence on projection), the coefficient value of the respective OPLS models, and IR database of compounds previously identified in OS, it was suggested that methoxy flavonoid (sinensitin and 5,6,7,3’-tetramethoxy-4’-hydroxy-8-C-preny-lflavone), diterpenes (orthosiphols, orthoarisins, neoorthosiphols, staminols, and staminolactones) and triterpenes (ursolic acid, oleanolic acid, betulinic acid, hydroxybetulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsible compounds for the α-glucosidase inhibitory activity. Meanwhile phenolic (rosmarinic acid), methoxy flavonoid (eupatorin, sinensetin, 5-hydroxy-6,7,3’,4’-tetramethoxyflavone, salvigenin, 6-hydroxy-5,7,3’-trimethoxyflavone and 5,6,7,3’-tetramethoxy-4’-hydroxy-8-C-prenylflavone), diterpenes (orthosiphols, orthoarisins, neoortho-siphols, staminols, and staminolactones) and triterpenes (ursolic acid, oleanolic acid, betulinic acid, hydroxybetulinic acid, maslinic acid, α-amyrin and β-amyrin) were identified as responsible compounds for the antioxidant activity.
format	Article
author	Juliani, J. Yuliana, Nancy Dewi Budijanto, Slamet Wijaya, C. Hanny Khatib, Alfi
author_facet	Juliani, J. Yuliana, Nancy Dewi Budijanto, Slamet Wijaya, C. Hanny Khatib, Alfi
author_sort	Juliani, J.
title	Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
title_short	Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
title_full	Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
title_fullStr	Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
title_full_unstemmed	Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics
title_sort	alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using ftir based metabolomics
publisher	Indonesian Food Technologist Association (IAFT)
publishDate	2016
url	http://irep.iium.edu.my/58994/ http://irep.iium.edu.my/58994/ http://irep.iium.edu.my/58994/1/juliani%20et%20al.%20%282016%29.pdf
first_indexed	2023-09-18T21:23:30Z
last_indexed	2023-09-18T21:23:30Z
_version_	1777412038065127424

Alpha-glucosidase inhibitor and anti-oxidant compounds from orthosiphon stamineus benth using FTIR based metabolomics

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