Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities

The phytochemical and biological activity of the leaves and stem barks of Garcinia griffithii have been investigated. Extraction and chromatographic purification of the leaves and stem barks extracts have successfully afforded five biflavonoids namely amento- 4’’-methylether (1), 3,8’’-binaringe...

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Main Authors:	Wan Salleh, Wan Mohd Nuzul Hakimi, Nik Sazali, Nik Shazwani Afifah, Ahmad, Farediah, Bakhtiar, M. Taher
Format:	Article
Language:	English English
Published:	Marmara University Press 2017
Subjects:	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry
Online Access:	http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/1/G%20grifithii%20Marmara.pdf http://irep.iium.edu.my/58842/8/58442-Biflavonoids%20from%20the%20leaves%20and%20stem%20bark%20of%20Garcinia%20griffithii_SCOPUS.pdf

id	iium-58842
recordtype	eprints
spelling	iium-588422018-04-05T06:55:49Z http://irep.iium.edu.my/58842/ Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities Wan Salleh, Wan Mohd Nuzul Hakimi Nik Sazali, Nik Shazwani Afifah Ahmad, Farediah Bakhtiar, M. Taher RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry The phytochemical and biological activity of the leaves and stem barks of Garcinia griffithii have been investigated. Extraction and chromatographic purification of the leaves and stem barks extracts have successfully afforded five biflavonoids namely amento- 4’’-methylether (1), 3,8’’-binaringenin (2), morelloflavone (3), 3,8’’-binaringenin-7’’-O-glucoside (4) and morelloflavone-7’’-Oglucoside (5), together with squalene (6), canophyllol (7), friedelin (8) and β-amyrin (9) which were characterized spectroscopically. All extracts and phytochemicals were tested for antioxidant, antityrosinase and antibacterial activities. The antioxidant assay on DPPH radical scavenging showed that the n-hexane extract of the stem barks had the highest radical scavenging activity with IC50 value of 96.4 μg/mL, while compound (3) was found to be the strongest antioxidant compound with IC50 value of 57.6 μg/mL. The methanol extract of the leaves showed the highest total phenolic content with 444.1 mg/g of gallic acid equivalent (GAE/L) and 423.1 mg/g of cathechin equivalent (CE/L). The extracts and all compounds were found to have weak antityrosinase activity. The antimicrobial assays of all the extracts were carried out by minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The methanol extract of the leaves showed the most significant antimicrobial activity towards E. faecalis and K. pneumoniae with MIC and MBC value ranged between 225-450 μg/mL compared to the other extracts. Marmara University Press 2017-10 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58842/1/G%20grifithii%20Marmara.pdf application/pdf en http://irep.iium.edu.my/58842/8/58442-Biflavonoids%20from%20the%20leaves%20and%20stem%20bark%20of%20Garcinia%20griffithii_SCOPUS.pdf Wan Salleh, Wan Mohd Nuzul Hakimi and Nik Sazali, Nik Shazwani Afifah and Ahmad, Farediah and Bakhtiar, M. Taher (2017) Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities. Marmara Pharmaceutical Journal, 21 (4). pp. 889-897. ISSN 1309-0801 http://www.marmarapharmj.com/uploads/pdf/pdf_MPJ_560.pdf 10.12991/mpj.2017.27
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry
spellingShingle	RS Pharmacy and materia medica RS403 Materia Medica-Pharmaceutical Chemistry Wan Salleh, Wan Mohd Nuzul Hakimi Nik Sazali, Nik Shazwani Afifah Ahmad, Farediah Bakhtiar, M. Taher Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
description	The phytochemical and biological activity of the leaves and stem barks of Garcinia griffithii have been investigated. Extraction and chromatographic purification of the leaves and stem barks extracts have successfully afforded five biflavonoids namely amento- 4’’-methylether (1), 3,8’’-binaringenin (2), morelloflavone (3), 3,8’’-binaringenin-7’’-O-glucoside (4) and morelloflavone-7’’-Oglucoside (5), together with squalene (6), canophyllol (7), friedelin (8) and β-amyrin (9) which were characterized spectroscopically. All extracts and phytochemicals were tested for antioxidant, antityrosinase and antibacterial activities. The antioxidant assay on DPPH radical scavenging showed that the n-hexane extract of the stem barks had the highest radical scavenging activity with IC50 value of 96.4 μg/mL, while compound (3) was found to be the strongest antioxidant compound with IC50 value of 57.6 μg/mL. The methanol extract of the leaves showed the highest total phenolic content with 444.1 mg/g of gallic acid equivalent (GAE/L) and 423.1 mg/g of cathechin equivalent (CE/L). The extracts and all compounds were found to have weak antityrosinase activity. The antimicrobial assays of all the extracts were carried out by minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The methanol extract of the leaves showed the most significant antimicrobial activity towards E. faecalis and K. pneumoniae with MIC and MBC value ranged between 225-450 μg/mL compared to the other extracts.
format	Article
author	Wan Salleh, Wan Mohd Nuzul Hakimi Nik Sazali, Nik Shazwani Afifah Ahmad, Farediah Bakhtiar, M. Taher
author_facet	Wan Salleh, Wan Mohd Nuzul Hakimi Nik Sazali, Nik Shazwani Afifah Ahmad, Farediah Bakhtiar, M. Taher
author_sort	Wan Salleh, Wan Mohd Nuzul Hakimi
title	Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
title_short	Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
title_full	Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
title_fullStr	Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
title_full_unstemmed	Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
title_sort	biflavonoids from the leaves and stem bark of garcinia griffithii and their biological activities
publisher	Marmara University Press
publishDate	2017
url	http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/1/G%20grifithii%20Marmara.pdf http://irep.iium.edu.my/58842/8/58442-Biflavonoids%20from%20the%20leaves%20and%20stem%20bark%20of%20Garcinia%20griffithii_SCOPUS.pdf
first_indexed	2023-09-18T21:23:16Z
last_indexed	2023-09-18T21:23:16Z
_version_	1777412023069442048

Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities

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