Biflavonoids from the leaves and stem bark of Garcinia griffithii and their biological activities
The phytochemical and biological activity of the leaves and stem barks of Garcinia griffithii have been investigated. Extraction and chromatographic purification of the leaves and stem barks extracts have successfully afforded five biflavonoids namely amento- 4’’-methylether (1), 3,8’’-binaringe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Marmara University Press
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/ http://irep.iium.edu.my/58842/1/G%20grifithii%20Marmara.pdf http://irep.iium.edu.my/58842/8/58442-Biflavonoids%20from%20the%20leaves%20and%20stem%20bark%20of%20Garcinia%20griffithii_SCOPUS.pdf |
| Summary: | The phytochemical and biological activity of the leaves and stem
barks of Garcinia griffithii have been investigated. Extraction and
chromatographic purification of the leaves and stem barks extracts
have successfully afforded five biflavonoids namely amento-
4’’-methylether (1), 3,8’’-binaringenin (2), morelloflavone (3),
3,8’’-binaringenin-7’’-O-glucoside (4) and morelloflavone-7’’-Oglucoside
(5), together with squalene (6), canophyllol (7), friedelin
(8) and β-amyrin (9) which were characterized spectroscopically.
All extracts and phytochemicals were tested for antioxidant,
antityrosinase and antibacterial activities. The antioxidant assay
on DPPH radical scavenging showed that the n-hexane extract
of the stem barks had the highest radical scavenging activity
with IC50 value of 96.4 μg/mL, while compound (3) was found
to be the strongest antioxidant compound with IC50 value of 57.6
μg/mL. The methanol extract of the leaves showed the highest
total phenolic content with 444.1 mg/g of gallic acid equivalent
(GAE/L) and 423.1 mg/g of cathechin equivalent (CE/L). The
extracts and all compounds were found to have weak antityrosinase
activity. The antimicrobial assays of all the extracts
were carried out by minimum inhibition concentration (MIC)
and minimum bactericidal concentration (MBC). The methanol
extract of the leaves showed the most significant antimicrobial
activity towards E. faecalis and K. pneumoniae with MIC and
MBC value ranged between 225-450 μg/mL compared to the
other extracts. |
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