Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells

Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride dihydrate (CaCl2.2H2O) of white...

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Main Authors:	Ahmed, Hany Mohammed Aly, Luddin, Norhayati, Kannan, Thirumulu Ponnuraj, Mokhtar@Makhtar, Khairani Idah, Ahmad, Azlina
Format:	Article
Language:	English English
Published:	Wolters Kluwer - Medknow 2017
Subjects:	RK Dentistry
Online Access:	http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/1/Ahmed%20et%20al%202017%20ejgd.pdf http://irep.iium.edu.my/58835/7/Dentinogenic%20Differentiation%20Potential%20of%20Fast%20Set.pdf

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recordtype	eprints
spelling	iium-588352018-04-17T08:40:38Z http://irep.iium.edu.my/58835/ Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells Ahmed, Hany Mohammed Aly Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina RK Dentistry Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride dihydrate (CaCl2.2H2O) of white Portland cements (WPCs) of a different origin (Aalborg, Malaysia, and Egypt) on dental pulp stem cells (DPSCs). Materials and Methods: The material extracts at 12.5 mg/ml were applied on DPSCs cultured in 96‑well plates. After 1, 3, 7 and 14 days of incubation, the RNA was extracted, cDNA was prepared, and the expression of four dentinogenic gene markers (bone gamma‑carboxyglutamate protein, dentin sialophosphoprotein, runt‑related transcription factor 2, and secreted phosphoprotein 1 [SPP1]) was examined using the real‑time polymerase chain reaction. One‑way analysis of variance was used for statistical analysis, and the level of significance was set at 0.05 (P = 0.05). Results: Significant differences were observed between Malaysian WPC (MAWPC) and Egyptian WPC (EGWPC) and FS MAWPC), FS EGWPC in 7 out of 15 and 6 out of 10 comparisons, respectively. While more expressions in EGWPC group were observed in four comparisons and three for MAWPC, all FS formulations showed higher expressions for FS MAWPC compared to FS EGWPC (P < 0.05). The addition of CaCl2.2H2O to MAWPC and EGWPC increased the upregulation of SPP1 gene at all‑day intervals, which was not observed with other genes. Conclusions: The dentinogenic differentiation of DPSCs shows different expression profiles to extracts of normal and fast formulations of WPC. Extracts of WPC of different origin have different dentinogenic differentiation potential on DPSCs. Keywords: Calcium chloride dihydrate, dentinogenic differentiation, Egypt, Malaysia, real‑time polymerase chain reaction, white Portland cement Wolters Kluwer - Medknow 2017-09-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58835/1/Ahmed%20et%20al%202017%20ejgd.pdf application/pdf en http://irep.iium.edu.my/58835/7/Dentinogenic%20Differentiation%20Potential%20of%20Fast%20Set.pdf Ahmed, Hany Mohammed Aly and Luddin, Norhayati and Kannan, Thirumulu Ponnuraj and Mokhtar@Makhtar, Khairani Idah and Ahmad, Azlina (2017) Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells. European Journal of General Dentistry, 6 (3). pp. 115-122. ISSN 2278-9626 E-ISSN 2320-4753 http://www.ejgd.org/article.asp?issn=2278-9626;year=2017;volume=6;issue=3;spage=115;epage=122;aulast=Ahmed 10.4103/ejgd.ejgd_42_17
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK Dentistry
spellingShingle	RK Dentistry Ahmed, Hany Mohammed Aly Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
description	Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride dihydrate (CaCl2.2H2O) of white Portland cements (WPCs) of a different origin (Aalborg, Malaysia, and Egypt) on dental pulp stem cells (DPSCs). Materials and Methods: The material extracts at 12.5 mg/ml were applied on DPSCs cultured in 96‑well plates. After 1, 3, 7 and 14 days of incubation, the RNA was extracted, cDNA was prepared, and the expression of four dentinogenic gene markers (bone gamma‑carboxyglutamate protein, dentin sialophosphoprotein, runt‑related transcription factor 2, and secreted phosphoprotein 1 [SPP1]) was examined using the real‑time polymerase chain reaction. One‑way analysis of variance was used for statistical analysis, and the level of significance was set at 0.05 (P = 0.05). Results: Significant differences were observed between Malaysian WPC (MAWPC) and Egyptian WPC (EGWPC) and FS MAWPC), FS EGWPC in 7 out of 15 and 6 out of 10 comparisons, respectively. While more expressions in EGWPC group were observed in four comparisons and three for MAWPC, all FS formulations showed higher expressions for FS MAWPC compared to FS EGWPC (P < 0.05). The addition of CaCl2.2H2O to MAWPC and EGWPC increased the upregulation of SPP1 gene at all‑day intervals, which was not observed with other genes. Conclusions: The dentinogenic differentiation of DPSCs shows different expression profiles to extracts of normal and fast formulations of WPC. Extracts of WPC of different origin have different dentinogenic differentiation potential on DPSCs. Keywords: Calcium chloride dihydrate, dentinogenic differentiation, Egypt, Malaysia, real‑time polymerase chain reaction, white Portland cement
format	Article
author	Ahmed, Hany Mohammed Aly Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_facet	Ahmed, Hany Mohammed Aly Luddin, Norhayati Kannan, Thirumulu Ponnuraj Mokhtar@Makhtar, Khairani Idah Ahmad, Azlina
author_sort	Ahmed, Hany Mohammed Aly
title	Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
title_short	Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
title_full	Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
title_fullStr	Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
title_full_unstemmed	Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
title_sort	dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
publisher	Wolters Kluwer - Medknow
publishDate	2017
url	http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/1/Ahmed%20et%20al%202017%20ejgd.pdf http://irep.iium.edu.my/58835/7/Dentinogenic%20Differentiation%20Potential%20of%20Fast%20Set.pdf
first_indexed	2023-09-18T21:23:15Z
last_indexed	2023-09-18T21:23:15Z
_version_	1777412022331244544

Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells

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