Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells
Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride dihydrate (CaCl2.2H2O) of white...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Wolters Kluwer - Medknow
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/ http://irep.iium.edu.my/58835/1/Ahmed%20et%20al%202017%20ejgd.pdf http://irep.iium.edu.my/58835/7/Dentinogenic%20Differentiation%20Potential%20of%20Fast%20Set.pdf |
| Summary: | Background: Advances in endodontic biomaterials are at the forefront of endodontic research. Purpose: This study aimed to compare
the dentinogenic differentiation potential of extracts of two formulations (normal and fast set [FS] by the addition of calcium chloride
dihydrate (CaCl2.2H2O) of white Portland cements (WPCs) of a different origin (Aalborg, Malaysia, and Egypt) on dental pulp stem cells (DPSCs).
Materials and Methods: The material extracts at 12.5 mg/ml were applied on DPSCs cultured in 96‑well plates. After 1, 3, 7 and 14 days of
incubation, the RNA was extracted, cDNA was prepared, and the expression of four dentinogenic gene markers (bone gamma‑carboxyglutamate
protein, dentin sialophosphoprotein, runt‑related transcription factor 2, and secreted phosphoprotein 1 [SPP1]) was examined using the
real‑time polymerase chain reaction. One‑way analysis of variance was used for statistical analysis, and the level of significance was set at
0.05 (P = 0.05). Results: Significant differences were observed between Malaysian WPC (MAWPC) and Egyptian WPC (EGWPC) and FS
MAWPC), FS EGWPC in 7 out of 15 and 6 out of 10 comparisons, respectively. While more expressions in EGWPC group were observed in
four comparisons and three for MAWPC, all FS formulations showed higher expressions for FS MAWPC compared to FS EGWPC (P < 0.05).
The addition of CaCl2.2H2O to MAWPC and EGWPC increased the upregulation of SPP1 gene at all‑day intervals, which was not observed
with other genes. Conclusions: The dentinogenic differentiation of DPSCs shows different expression profiles to extracts of normal and fast
formulations of WPC. Extracts of WPC of different origin have different dentinogenic differentiation potential on DPSCs.
Keywords: Calcium chloride dihydrate, dentinogenic differentiation, Egypt, Malaysia, real‑time polymerase chain reaction, white Portland cement |
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