Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions

Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone developmen...

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Main Authors:	Mokhtar@Makhtar, Khairani Idah, Abu Bakar, Noraini, Kharuddin, Azrul Fazwan
Format:	Conference or Workshop Item
Language:	English English
Published:	2017
Subjects:	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Online Access:	http://irep.iium.edu.my/58834/ http://irep.iium.edu.my/58834/ http://irep.iium.edu.my/58834/2/KIM-IIDC%202017%20POSTER-Determination%20of%20RUNX2%20SNP%20rs6930053%20in%20Class%20I%2CII%20and%20III%20malocclusions.pdf http://irep.iium.edu.my/58834/9/58834_abstract.pdf

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spelling	iium-588342018-05-17T06:36:23Z http://irep.iium.edu.my/58834/ Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone development disorder, while single nucleotide polymorphism (SNP) in RUNX2 is associated with Class II/2 malocclusion. This study aimed to determine RUNX2 SNP of DNA marker (rs6930053) in malocclusion patients from local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 Class I (control samples), 30 Class II and 30 Class III malocclusion patients. Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for marker rs6930053 and the genotyping was done by sequencing. Chi-square test was used to determine differences in allele and genotype frequencies (p<0.05). Results: No significant differences were observed in RUNX2 SNP (rs8004560) in Class I and Class III malocclusion. However, there were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within Class II alone; while significant differences was detected only in allele frequency between control and Class II malocclusion (p=0.019). Conclusion(s): There is genetic association between RUNX2 (rs6930053) in Class II malocclusion in our population. Further studies involving larger number of samples and other DNA markers of RUNX2 gene should be developed in order to understand the exact role and mechanism of RUNX2 in different classes of malocclusions. 2017-10-06 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/58834/2/KIM-IIDC%202017%20POSTER-Determination%20of%20RUNX2%20SNP%20rs6930053%20in%20Class%20I%2CII%20and%20III%20malocclusions.pdf application/pdf en http://irep.iium.edu.my/58834/9/58834_abstract.pdf Mokhtar@Makhtar, Khairani Idah and Abu Bakar, Noraini and Kharuddin, Azrul Fazwan (2017) Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions. In: 1st IUM International Dental Conference 2017 (IIDC), 6th-8th Oct. 2017, Zenith Hotel, Kuantan, Pahang. (Unpublished) http://iidc.com.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
spellingShingle	RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
description	Introduction: Runt-related transcription factor 2 (RUNX2) plays important roles in osteoblast differentiation, tooth development and chondrocyte maturation; hence its involvement in craniofacial development is paramount. Mutation in RUNX2 is implicated with cleidocranial dysplasia; a bone development disorder, while single nucleotide polymorphism (SNP) in RUNX2 is associated with Class II/2 malocclusion. This study aimed to determine RUNX2 SNP of DNA marker (rs6930053) in malocclusion patients from local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 Class I (control samples), 30 Class II and 30 Class III malocclusion patients. Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for marker rs6930053 and the genotyping was done by sequencing. Chi-square test was used to determine differences in allele and genotype frequencies (p<0.05). Results: No significant differences were observed in RUNX2 SNP (rs8004560) in Class I and Class III malocclusion. However, there were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within Class II alone; while significant differences was detected only in allele frequency between control and Class II malocclusion (p=0.019). Conclusion(s): There is genetic association between RUNX2 (rs6930053) in Class II malocclusion in our population. Further studies involving larger number of samples and other DNA markers of RUNX2 gene should be developed in order to understand the exact role and mechanism of RUNX2 in different classes of malocclusions.
format	Conference or Workshop Item
author	Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan
author_facet	Mokhtar@Makhtar, Khairani Idah Abu Bakar, Noraini Kharuddin, Azrul Fazwan
author_sort	Mokhtar@Makhtar, Khairani Idah
title	Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
title_short	Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
title_full	Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
title_fullStr	Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
title_full_unstemmed	Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions
title_sort	determination of runx2 single nucleotide polymorphism rs6930053 in class i, ii and iii malocclusions
publishDate	2017
url	http://irep.iium.edu.my/58834/ http://irep.iium.edu.my/58834/ http://irep.iium.edu.my/58834/2/KIM-IIDC%202017%20POSTER-Determination%20of%20RUNX2%20SNP%20rs6930053%20in%20Class%20I%2CII%20and%20III%20malocclusions.pdf http://irep.iium.edu.my/58834/9/58834_abstract.pdf
first_indexed	2023-09-18T21:23:15Z
last_indexed	2023-09-18T21:23:15Z
_version_	1777412022195978240

Determination of RUNX2 single nucleotide polymorphism rs6930053 in class I, II and III malocclusions

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