Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment

Abstract: Xylanase is the key enzyme that involve in the hydrolysis of xylan, the main constituent of the complex hemicellulose of the plant cell wall. In this study, forty actinomycetes that were isolated from sediment of Kuantan Mangrove Forest, Malaysia, were tested for their ability to produce e...

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Main Authors:	Mohd Omar, Suhaila, Mohd Farouk, Norsyafawati, Abdul Malek, Nurfathiah, Zainal Abidin, Zaima Azira
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/58624/ http://irep.iium.edu.my/58624/ http://irep.iium.edu.my/58624/1/58624_Verrucosispora%20sp.%20K2-04%2C%20Potential%20Xylanase.pdf

id	iium-58624
recordtype	eprints
spelling	iium-586242017-12-15T00:51:27Z http://irep.iium.edu.my/58624/ Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment Mohd Omar, Suhaila Mohd Farouk, Norsyafawati Abdul Malek, Nurfathiah Zainal Abidin, Zaima Azira QR Microbiology Abstract: Xylanase is the key enzyme that involve in the hydrolysis of xylan, the main constituent of the complex hemicellulose of the plant cell wall. In this study, forty actinomycetes that were isolated from sediment of Kuantan Mangrove Forest, Malaysia, were tested for their ability to produce extracellular xylanase. At least 15 isolates were able to degrade xylan in the primary agar-based screening using marine agar containing 0.1% (v/v) azo-xylan (Birchwood). The degradation of xylan was indicated by the formation of halo zone around the colonies and the clear zone index (CZI) was calculated as a ratio of the clearing zones to the colony size. Isolate K2-04 with CZI 3.35 ± 1.91 was identified through 16S rRNA study as Verrucosispora sp. This isolate was further grown in marine broth and incubated at 30 °C, 200 rpm for 20 days. The growth of K2-04 and the xylanase activity was measured at day 2, 4, 6, 12 and 18 respectively. The highest enzyme activity for the crude enzyme was recorded at day 18 (1.836 U/mL) and exhibited stability after 20 days storage at 4°C. This study serves as a preliminary study to characterize the properties of Verrucosispora sp. K2-04, rare actinomycete of Kuantan Mangrove Forest, Malaysia. 2017-04-11 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/58624/1/58624_Verrucosispora%20sp.%20K2-04%2C%20Potential%20Xylanase.pdf Mohd Omar, Suhaila and Mohd Farouk, Norsyafawati and Abdul Malek, Nurfathiah and Zainal Abidin, Zaima Azira (2017) Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment. In: 2017 SEOUL Conference, 11-13th April 2017, Han Yang University, Seoul, South Korea. (Unpublished) http://www.icese.org/ICESE2017-program.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohd Omar, Suhaila Mohd Farouk, Norsyafawati Abdul Malek, Nurfathiah Zainal Abidin, Zaima Azira Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
description	Abstract: Xylanase is the key enzyme that involve in the hydrolysis of xylan, the main constituent of the complex hemicellulose of the plant cell wall. In this study, forty actinomycetes that were isolated from sediment of Kuantan Mangrove Forest, Malaysia, were tested for their ability to produce extracellular xylanase. At least 15 isolates were able to degrade xylan in the primary agar-based screening using marine agar containing 0.1% (v/v) azo-xylan (Birchwood). The degradation of xylan was indicated by the formation of halo zone around the colonies and the clear zone index (CZI) was calculated as a ratio of the clearing zones to the colony size. Isolate K2-04 with CZI 3.35 ± 1.91 was identified through 16S rRNA study as Verrucosispora sp. This isolate was further grown in marine broth and incubated at 30 °C, 200 rpm for 20 days. The growth of K2-04 and the xylanase activity was measured at day 2, 4, 6, 12 and 18 respectively. The highest enzyme activity for the crude enzyme was recorded at day 18 (1.836 U/mL) and exhibited stability after 20 days storage at 4°C. This study serves as a preliminary study to characterize the properties of Verrucosispora sp. K2-04, rare actinomycete of Kuantan Mangrove Forest, Malaysia.
format	Conference or Workshop Item
author	Mohd Omar, Suhaila Mohd Farouk, Norsyafawati Abdul Malek, Nurfathiah Zainal Abidin, Zaima Azira
author_facet	Mohd Omar, Suhaila Mohd Farouk, Norsyafawati Abdul Malek, Nurfathiah Zainal Abidin, Zaima Azira
author_sort	Mohd Omar, Suhaila
title	Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
title_short	Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
title_full	Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
title_fullStr	Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
title_full_unstemmed	Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment
title_sort	verrucosispora sp. k2-04, potential xylanase producer from kuantan mangrove forest sediment
publishDate	2017
url	http://irep.iium.edu.my/58624/ http://irep.iium.edu.my/58624/ http://irep.iium.edu.my/58624/1/58624_Verrucosispora%20sp.%20K2-04%2C%20Potential%20Xylanase.pdf
first_indexed	2023-09-18T21:22:56Z
last_indexed	2023-09-18T21:22:56Z
_version_	1777412002422980608

Verrucosispora sp. K2-04, potential xylanase producer from Kuantan Mangrove Forest sediment

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