Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients

Background The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nep...

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Main Authors:	Mehde, Atheer Awad, Mehdi, Wesen Adel, Yusof, Faridah, Ahmad Raus, Raha, Zainal Abidin, Zaima Azira, Ghazali, Hamid, Abd Rahman, Azlina
Format:	Article
Language:	English English English
Published:	Elsevier B.V. 2017
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/1/P%2091.pdf http://irep.iium.edu.my/58529/7/58529_Determination%20of%20alpha-2-MRAP%20gene_MYRA.pdf http://irep.iium.edu.my/58529/8/58529_Determination%20of%20alpha-2-MRAP%20gene_SCOPUS.pdf

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spelling	iium-585292018-03-19T04:31:07Z http://irep.iium.edu.my/58529/ Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Ahmad Raus, Raha Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina R Medicine (General) Background The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients. Methods PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined. Results The D and I allele frequencies were 57.50% and 42.50% in patients, 77.50% and 22.50% in control, individually. The result showed that II genotype was associated with nephrolithiasis patients group. A significant decrease was observed in serum Alpha-2-MRAP,myeloperoxidase and TAS,while TOS,OSI,MDA,AOPP and uric acid were substantially increased in II and ID when compared to DD genotype in patients with nephrolithiasis. Conclusion Our results demonstrate for the first time that patients with II genotype had an increased risk of stones. Also, the results demonstrate that I allele of the 5 insertion/deletion polymorphism in the Alpha-2-MRAP gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with II genotype of Alpha-2-MRAP. Elsevier B.V. 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58529/1/P%2091.pdf application/pdf en http://irep.iium.edu.my/58529/7/58529_Determination%20of%20alpha-2-MRAP%20gene_MYRA.pdf application/pdf en http://irep.iium.edu.my/58529/8/58529_Determination%20of%20alpha-2-MRAP%20gene_SCOPUS.pdf Mehde, Atheer Awad and Mehdi, Wesen Adel and Yusof, Faridah and Ahmad Raus, Raha and Zainal Abidin, Zaima Azira and Ghazali, Hamid and Abd Rahman, Azlina (2017) Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients. International Journal of Biological Macromolecules, 105 (Part 1). pp. 1324-1327. ISSN 0141-8130 https://www.sciencedirect.com/science/article/pii/S0141813017310115?via%3Dihub 10.1016/j.ijbiomac.2017.07.167
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Ahmad Raus, Raha Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
description	Background The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients. Methods PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined. Results The D and I allele frequencies were 57.50% and 42.50% in patients, 77.50% and 22.50% in control, individually. The result showed that II genotype was associated with nephrolithiasis patients group. A significant decrease was observed in serum Alpha-2-MRAP,myeloperoxidase and TAS,while TOS,OSI,MDA,AOPP and uric acid were substantially increased in II and ID when compared to DD genotype in patients with nephrolithiasis. Conclusion Our results demonstrate for the first time that patients with II genotype had an increased risk of stones. Also, the results demonstrate that I allele of the 5 insertion/deletion polymorphism in the Alpha-2-MRAP gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with II genotype of Alpha-2-MRAP.
format	Article
author	Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Ahmad Raus, Raha Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina
author_facet	Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Ahmad Raus, Raha Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina
author_sort	Mehde, Atheer Awad
title	Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
title_short	Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
title_full	Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
title_fullStr	Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
title_full_unstemmed	Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
title_sort	determination of alpha-2-mrap gene polymorphisms innephrolithiasis patients
publisher	Elsevier B.V.
publishDate	2017
url	http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/1/P%2091.pdf http://irep.iium.edu.my/58529/7/58529_Determination%20of%20alpha-2-MRAP%20gene_MYRA.pdf http://irep.iium.edu.my/58529/8/58529_Determination%20of%20alpha-2-MRAP%20gene_SCOPUS.pdf
first_indexed	2023-09-18T21:22:46Z
last_indexed	2023-09-18T21:22:46Z
_version_	1777411992130158592

Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients

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