Determination of alpha-2-MRAP gene polymorphisms innephrolithiasis patients
Background The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nep...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Elsevier B.V.
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/ http://irep.iium.edu.my/58529/1/P%2091.pdf http://irep.iium.edu.my/58529/7/58529_Determination%20of%20alpha-2-MRAP%20gene_MYRA.pdf http://irep.iium.edu.my/58529/8/58529_Determination%20of%20alpha-2-MRAP%20gene_SCOPUS.pdf |
| Summary: | Background
The intron 5 insertion/deletion polymorphism of Alpha-2-macroglobulin receptor-associated protein gene (Alpha-2-MRAP) has been implicated in numerous diseases. The current study was designed to analyze the association of intron 5 insertion/deletion polymorphism of Alpha-2-MRAP with nephrolithiasis patients.
Methods
PCR was conducted on genomic DNA of patients and control to look for Alpha-2-MRAP insertion/deletion polymorphism. Besides that, serum level of Alpha-2-MRAP, oxidative stress marker myeloperoxidase, Malondialdehyde (MDA), Advanced oxidation protein products (AOPP), and uric acid were determined.
Results
The D and I allele frequencies were 57.50% and 42.50% in patients, 77.50% and 22.50% in control, individually. The result showed that II genotype was associated with nephrolithiasis patients group. A significant decrease was observed in serum Alpha-2-MRAP,myeloperoxidase and TAS,while TOS,OSI,MDA,AOPP and uric acid were substantially increased in II and ID when compared to DD genotype in patients with nephrolithiasis.
Conclusion
Our results demonstrate for the first time that patients with II genotype had an increased risk of stones. Also, the results demonstrate that I allele of the 5 insertion/deletion polymorphism in the Alpha-2-MRAP gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with II genotype of Alpha-2-MRAP. |
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