Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate

A laboratory-scale lignin peroxidase (LiP) or ligninase production from palm oil mill effluent (POME) by Phanerochaete chrysosporium in liquid state bioconversion (LSB) was studied. The effect of the concentration of substrate and co-substrate, inoculum size, initial pH of the medium, and the agitat...

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Main Authors:	Alam, Md. Zahangir, Abdul Karim, Mohamed Ismail, Mespuan, M T
Format:	Conference or Workshop Item
Language:	English
Published:	2006
Subjects:	TP Chemical technology TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5851/ http://irep.iium.edu.my/5851/1/Somche-2.pdf

id	iium-5851
recordtype	eprints
spelling	iium-58512011-11-21T22:45:04Z http://irep.iium.edu.my/5851/ Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Mespuan, M T TP Chemical technology TP248.13 Biotechnology A laboratory-scale lignin peroxidase (LiP) or ligninase production from palm oil mill effluent (POME) by Phanerochaete chrysosporium in liquid state bioconversion (LSB) was studied. The effect of the concentration of substrate and co-substrate, inoculum size, initial pH of the medium, and the agitation speed were observed for maximum production of LiP. The statistical optimization of the physicochemical conditions by polynomial regression model through the effect of linear, quadratic and polynomial interaction were carried out for the enzyme production. The experimental design and optimization was done by using the fractional factorial design formulated by a statistical optimization software MINITAB. The lignin peroxidase activity was observed periodically by the enzyme assaying and other responses such as COD, TSS, as well as reducing sugar determination. The results showed that temperature of 40°C, substrate concentration of 2%, co-substrate concentration of 2%, pH 6, inoculum size of 2%, and agitation of 250 rpm maximize the LiP production in liquid state bioconversion of POME. 2006-12-19 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/5851/1/Somche-2.pdf Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Mespuan, M T (2006) Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate. In: 20th Symposium of Malaysian Chemical Engineers (SOMChE 2006), 19-21 December 2006, Shah Alam.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP Chemical technology TP248.13 Biotechnology
spellingShingle	TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Mespuan, M T Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
description	A laboratory-scale lignin peroxidase (LiP) or ligninase production from palm oil mill effluent (POME) by Phanerochaete chrysosporium in liquid state bioconversion (LSB) was studied. The effect of the concentration of substrate and co-substrate, inoculum size, initial pH of the medium, and the agitation speed were observed for maximum production of LiP. The statistical optimization of the physicochemical conditions by polynomial regression model through the effect of linear, quadratic and polynomial interaction were carried out for the enzyme production. The experimental design and optimization was done by using the fractional factorial design formulated by a statistical optimization software MINITAB. The lignin peroxidase activity was observed periodically by the enzyme assaying and other responses such as COD, TSS, as well as reducing sugar determination. The results showed that temperature of 40°C, substrate concentration of 2%, co-substrate concentration of 2%, pH 6, inoculum size of 2%, and agitation of 250 rpm maximize the LiP production in liquid state bioconversion of POME.
format	Conference or Workshop Item
author	Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Mespuan, M T
author_facet	Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Mespuan, M T
author_sort	Alam, Md. Zahangir
title	Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
title_short	Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
title_full	Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
title_fullStr	Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
title_full_unstemmed	Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
title_sort	statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate
publishDate	2006
url	http://irep.iium.edu.my/5851/ http://irep.iium.edu.my/5851/1/Somche-2.pdf
first_indexed	2023-09-18T20:14:38Z
last_indexed	2023-09-18T20:14:38Z
_version_	1777407705589219328

Statistical optimization of physicochemical factors for lignin peroxidase production using pome as substrate

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