Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed

This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three signifcant factors (hydrolysis time, enzyme concentration and extraction sta...

Full description

Bibliographic Details
Main Authors: Hammed, Ademola Monsur, Jaswir, Irwandi, Senay, Simsek, Alam, Md Zahangir, Amid, Azura
Format: Article
Language:English
English
Published: Universiti Putra Malaysia 2017
Subjects:
Online Access:http://irep.iium.edu.my/58457/
http://irep.iium.edu.my/58457/
http://irep.iium.edu.my/58457/1/58457_Enzyme-aided%20extraction%20of%20sulfated.pdf
http://irep.iium.edu.my/58457/2/58457_Enzyme-aided%20extraction%20of%20sulfated_SCOPUS.pdf
id iium-58457
recordtype eprints
spelling iium-584572018-03-20T05:11:09Z http://irep.iium.edu.my/58457/ Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed Hammed, Ademola Monsur Jaswir, Irwandi Senay, Simsek Alam, Md Zahangir Amid, Azura TP248.13 Biotechnology This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three signifcant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused an increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2, hydrolysis time of 19.5 h and enzyme concentration of 1.5 μl/ml to produce 25.13% yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications. Universiti Putra Malaysia 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58457/1/58457_Enzyme-aided%20extraction%20of%20sulfated.pdf application/pdf en http://irep.iium.edu.my/58457/2/58457_Enzyme-aided%20extraction%20of%20sulfated_SCOPUS.pdf Hammed, Ademola Monsur and Jaswir, Irwandi and Senay, Simsek and Alam, Md Zahangir and Amid, Azura (2017) Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed. International Food Research Journal, 24 (4). pp. 1660-1666. ISSN 1985 4668 E-ISSN 2231 7546 http://www.ifrj.upm.edu.my/24%20(04)%202017/(42).pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Hammed, Ademola Monsur
Jaswir, Irwandi
Senay, Simsek
Alam, Md Zahangir
Amid, Azura
Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
description This study involves extraction of sulfated polysaccahride (SP) from brown seaweed (Turbinaria turbinata). Eight processing conditions affecting enzyme aided extraction (EAE) were screened using Plackett-Burman design. Three signifcant factors (hydrolysis time, enzyme concentration and extraction stage) were optimized using Faced Centred Central Composite Design in Random Surface Methods. Micrograph obtained using Field Emission Scanning Electron Microscopy revealed that cellulase degradation ruptured the seaweed cell matrix thus caused an increase in the release of SP. The optimum conditions for extraction of SP from T. turbinata are: extraction stage of 2, hydrolysis time of 19.5 h and enzyme concentration of 1.5 μl/ml to produce 25.13% yield. The SP obtained from cellulase treated T. turbinata is a suitable anti-inflammatory agent for pharmaceutical applications.
format Article
author Hammed, Ademola Monsur
Jaswir, Irwandi
Senay, Simsek
Alam, Md Zahangir
Amid, Azura
author_facet Hammed, Ademola Monsur
Jaswir, Irwandi
Senay, Simsek
Alam, Md Zahangir
Amid, Azura
author_sort Hammed, Ademola Monsur
title Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
title_short Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
title_full Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
title_fullStr Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
title_full_unstemmed Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
title_sort enzyme-aided extraction of sulfated polysaccharides from turbinaria turbinata brown seaweed
publisher Universiti Putra Malaysia
publishDate 2017
url http://irep.iium.edu.my/58457/
http://irep.iium.edu.my/58457/
http://irep.iium.edu.my/58457/1/58457_Enzyme-aided%20extraction%20of%20sulfated.pdf
http://irep.iium.edu.my/58457/2/58457_Enzyme-aided%20extraction%20of%20sulfated_SCOPUS.pdf
first_indexed 2023-09-18T21:22:40Z
last_indexed 2023-09-18T21:22:40Z
_version_ 1777411985736990720