First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR

First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR

Objective: To identify 18S ribosomal RNA partial sequences from three different points, namely downstream, midstream and upstream of two water rivers in Kuantan, Pahang, Malaysia Methods: In this study, a total of six water samples were collected from three different points of both Kuantan River and...

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Main Authors: Barudin, Mohd Aiman, Md Isa, Muhammad Lokman, Othman, Noratikah, Mat Yusof, Afzan
Format: Article
Language:English
English
Published: Hainan Medical University 2017
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/7/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers.pdf
http://irep.iium.edu.my/58354/8/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers_SCOPUS.pdf
id iium-58354
recordtype eprints
spelling iium-583542018-04-25T09:14:38Z http://irep.iium.edu.my/58354/ First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR Barudin, Mohd Aiman Md Isa, Muhammad Lokman Othman, Noratikah Mat Yusof, Afzan Q Science (General) Objective: To identify 18S ribosomal RNA partial sequences from three different points, namely downstream, midstream and upstream of two water rivers in Kuantan, Pahang, Malaysia Methods: In this study, a total of six water samples were collected from three different points of both Kuantan River and Balok River. Each water sample was processed and concentrated using continuous flow centrifuge (CFC) machine and purified using immunomagnetic separation (IMS) technique. Nested Polymerase Chain Reaction (nPCR) was applied to detect 18S ribosomal RNA sequence in those samples after DNA extraction. Genotyping and phylogenetic analysis were performed on the basis of 18S rDNA gene sequence of Cryptosporidium. Results: A total of five samples from six different points of both Kuantan River and Balok River were contaminated with Cryptosporidium. Only river water sample from the upstream point of Kuantan River was negative for Cryptosporidium. In this study, the sequencing results of five samples from both Kuantan River and Balok River belonged to Cryptosporidium parvum (C. parvum). Conclusions: To the best of our knowledge, this is the first report of C. parvum genotyping in both Kuantan River and Balok River by using molecular approach nested polymerase chain reaction (nPCR) on 18S ribosomal RNA gene. Hainan Medical University 2017-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58354/7/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers.pdf application/pdf en http://irep.iium.edu.my/58354/8/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers_SCOPUS.pdf Barudin, Mohd Aiman and Md Isa, Muhammad Lokman and Othman, Noratikah and Mat Yusof, Afzan (2017) First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR. Asian Pacific Journal of Tropical Disease, 7 (9). pp. 548-552. ISSN 2222-1808 http://oaji.net/articles/2017/3556-1503906951.pdf 10.12980/apjtd.7.2017D7-136
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Barudin, Mohd Aiman
Md Isa, Muhammad Lokman
Othman, Noratikah
Mat Yusof, Afzan
First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
description Objective: To identify 18S ribosomal RNA partial sequences from three different points, namely downstream, midstream and upstream of two water rivers in Kuantan, Pahang, Malaysia Methods: In this study, a total of six water samples were collected from three different points of both Kuantan River and Balok River. Each water sample was processed and concentrated using continuous flow centrifuge (CFC) machine and purified using immunomagnetic separation (IMS) technique. Nested Polymerase Chain Reaction (nPCR) was applied to detect 18S ribosomal RNA sequence in those samples after DNA extraction. Genotyping and phylogenetic analysis were performed on the basis of 18S rDNA gene sequence of Cryptosporidium. Results: A total of five samples from six different points of both Kuantan River and Balok River were contaminated with Cryptosporidium. Only river water sample from the upstream point of Kuantan River was negative for Cryptosporidium. In this study, the sequencing results of five samples from both Kuantan River and Balok River belonged to Cryptosporidium parvum (C. parvum). Conclusions: To the best of our knowledge, this is the first report of C. parvum genotyping in both Kuantan River and Balok River by using molecular approach nested polymerase chain reaction (nPCR) on 18S ribosomal RNA gene.
format Article
author Barudin, Mohd Aiman
Md Isa, Muhammad Lokman
Othman, Noratikah
Mat Yusof, Afzan
author_facet Barudin, Mohd Aiman
Md Isa, Muhammad Lokman
Othman, Noratikah
Mat Yusof, Afzan
author_sort Barudin, Mohd Aiman
title First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
title_short First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
title_full First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
title_fullStr First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
title_full_unstemmed First molecular characterization of Cryptosporidium from three different points of two main rivers in Kuantan, Malaysia using 18S rRNA gene nested PCR
title_sort first molecular characterization of cryptosporidium from three different points of two main rivers in kuantan, malaysia using 18s rrna gene nested pcr
publisher Hainan Medical University
publishDate 2017
url http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/
http://irep.iium.edu.my/58354/7/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers.pdf
http://irep.iium.edu.my/58354/8/58354-First%20molecular%20characterization%20of%20Cryptosporidium%20from%20three%20different%20points%20of%20two%20main%20rivers_SCOPUS.pdf
first_indexed 2023-09-18T21:22:31Z
last_indexed 2023-09-18T21:22:31Z
_version_ 1777411975879327744

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