Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses

The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Mur...

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Main Authors:	Shahidan, Norshazila, Othman, Rashidi, Jaswir, Irwandi, Hashim, Yumi Zuhanis Has-Yun
Format:	Article
Language:	English
Published:	Journal of Fundamental and Applied Sciences 2017
Subjects:	RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/1/JFAS%201.pdf

id	iium-58018
recordtype	eprints
spelling	iium-580182018-03-20T02:46:48Z http://irep.iium.edu.my/58018/ Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Murashige and Skoog Salt for control and half strength nutrients. Carotneoid content was determined using High Performance Liquid Chromatography. Under light condition, the value of lutein was in range 0.554 + 0.107 µg/g to 0.169+ 0.003 µg/g and β-carotene was in range of not detected up to 8.133 + 0.088 µg/g. Differ to dark condition, a trace amount of lutein was detected in each treatments and the highest amount of β-carotene was detected in Polyethylene glycol treatment (5.313 + 0.051 µg/g). Carotenoid content varies in each treatment due to adaptation of pumpkin calluses with abiotic stress induced to them. Journal of Fundamental and Applied Sciences 2017-08-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58018/1/JFAS%201.pdf Shahidan, Norshazila and Othman, Rashidi and Jaswir, Irwandi and Hashim, Yumi Zuhanis Has-Yun (2017) Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses. Journal of Fundamental and Applied Sciences, 9 (2S (Special issue)). pp. 861-875. ISSN 1112-9867 http://www.jfas.info/index.php/jfas/article/view/2708/1348 10.4314/jfas.v9i2s.56
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology
spellingShingle	RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
description	The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Murashige and Skoog Salt for control and half strength nutrients. Carotneoid content was determined using High Performance Liquid Chromatography. Under light condition, the value of lutein was in range 0.554 + 0.107 µg/g to 0.169+ 0.003 µg/g and β-carotene was in range of not detected up to 8.133 + 0.088 µg/g. Differ to dark condition, a trace amount of lutein was detected in each treatments and the highest amount of β-carotene was detected in Polyethylene glycol treatment (5.313 + 0.051 µg/g). Carotenoid content varies in each treatment due to adaptation of pumpkin calluses with abiotic stress induced to them.
format	Article
author	Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun
author_facet	Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun
author_sort	Shahidan, Norshazila
title	Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
title_short	Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
title_full	Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
title_fullStr	Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
title_full_unstemmed	Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
title_sort	effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (cucurbita moshata) calluses
publisher	Journal of Fundamental and Applied Sciences
publishDate	2017
url	http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/1/JFAS%201.pdf
first_indexed	2023-09-18T21:22:01Z
last_indexed	2023-09-18T21:22:01Z
_version_	1777411945274540032

Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses

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