Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result

INTRODUCTION: The prevalence of implant associated with infection remains high. The management of this incidence poses a major challenge in orthopaedic field since these scenarios are chronic and biofilm based. Colonization of bacterial and biofilm adhesion on implant may lead to infection at under...

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Main Authors:	Mohd Jan, Nurul Hafiza, Zulkifly, Ahmad Hafi, Ibrahim, Mohd Zulfadzli, Shaban, Munirah
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	RD701 Orthopedics
Online Access:	http://irep.iium.edu.my/57853/ http://irep.iium.edu.my/57853/ http://irep.iium.edu.my/57853/21/57853.pdf

id	iium-57853
recordtype	eprints
spelling	iium-578532017-08-04T01:31:27Z http://irep.iium.edu.my/57853/ Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafi Ibrahim, Mohd Zulfadzli Shaban, Munirah RD701 Orthopedics INTRODUCTION: The prevalence of implant associated with infection remains high. The management of this incidence poses a major challenge in orthopaedic field since these scenarios are chronic and biofilm based. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Surgically device removal and addition with prolonged used of antimicrobial theraphy are leads to patient suffering, substantial morbidity and even mortality. Hence, to overcome this, alternative treatment strategy using orthopaedic metallic implant coated with 3% silver was introduced. The 3% silver composite exhibits good bactericidal properties. It inhibits biofilm formation particularly, at the adhesion stage of the relevant bacteria. Hence, the aim of this study is to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing Staphylococcus aureus through in vitro experimental setting. METHODS: The antibacterial property of orthopaedic metal implants was investigated using Staphylococcus aureus ATCC 25923. The sterile nutrient agar media was prepared. The bacterial colony then was smeared on the surface of the agar media. The coated six holes mini plate and non coated six holes plate which served as control were placed on the surface of agar media. The samples then were incubated for culture under aerobic condition with temperature 37ºC for 24 hours and continued incubated up to 48 hours for assessment. In this study, the antibacterial effect was evaluated based on the diameter of inhibition zone using in situ inhibitory test. RESULTS: The in situ test showed that the colony was noted after 24 hours of incubation. After 48 hours of incubation, the inhibition zone was noted and the number colony did not increase compared to control sample. DISCUSSIONS: Considering the prolonged used of antibiotic as theraphy which leads to resistance, silver coating on orthopaedic implant become very attractive alternative as antibacterial agent. According to Chen et al., (2006) silver is the well known agent compared to other dopants as antibacterial since its character not only can inhibit the bacterial adhesion with broad antibacterial spectrum, but also consists long lasting antibacterial effect. This was proved from the outcome of this study which revealed positive response of the silver as antibacterial agent. The experiment works is still ongoing. CONCLUSION: This result suggested that test material of orthopaedic metal implant coated with silver composite has the intended potential as antibacterial and provide protection against medical device-related infection. 2017 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/57853/21/57853.pdf Mohd Jan, Nurul Hafiza and Zulkifly, Ahmad Hafi and Ibrahim, Mohd Zulfadzli and Shaban, Munirah (2017) Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result. In: Asia Pacific Orthopaedic Association Trauma and Infection Meeting 2017, 31st March - 2nd April 2017, Putrajaya. (Unpublished) http://www.apoatrauma.com/eposters.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RD701 Orthopedics
spellingShingle	RD701 Orthopedics Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafi Ibrahim, Mohd Zulfadzli Shaban, Munirah Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
description	INTRODUCTION: The prevalence of implant associated with infection remains high. The management of this incidence poses a major challenge in orthopaedic field since these scenarios are chronic and biofilm based. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Surgically device removal and addition with prolonged used of antimicrobial theraphy are leads to patient suffering, substantial morbidity and even mortality. Hence, to overcome this, alternative treatment strategy using orthopaedic metallic implant coated with 3% silver was introduced. The 3% silver composite exhibits good bactericidal properties. It inhibits biofilm formation particularly, at the adhesion stage of the relevant bacteria. Hence, the aim of this study is to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing Staphylococcus aureus through in vitro experimental setting. METHODS: The antibacterial property of orthopaedic metal implants was investigated using Staphylococcus aureus ATCC 25923. The sterile nutrient agar media was prepared. The bacterial colony then was smeared on the surface of the agar media. The coated six holes mini plate and non coated six holes plate which served as control were placed on the surface of agar media. The samples then were incubated for culture under aerobic condition with temperature 37ºC for 24 hours and continued incubated up to 48 hours for assessment. In this study, the antibacterial effect was evaluated based on the diameter of inhibition zone using in situ inhibitory test. RESULTS: The in situ test showed that the colony was noted after 24 hours of incubation. After 48 hours of incubation, the inhibition zone was noted and the number colony did not increase compared to control sample. DISCUSSIONS: Considering the prolonged used of antibiotic as theraphy which leads to resistance, silver coating on orthopaedic implant become very attractive alternative as antibacterial agent. According to Chen et al., (2006) silver is the well known agent compared to other dopants as antibacterial since its character not only can inhibit the bacterial adhesion with broad antibacterial spectrum, but also consists long lasting antibacterial effect. This was proved from the outcome of this study which revealed positive response of the silver as antibacterial agent. The experiment works is still ongoing. CONCLUSION: This result suggested that test material of orthopaedic metal implant coated with silver composite has the intended potential as antibacterial and provide protection against medical device-related infection.
format	Conference or Workshop Item
author	Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafi Ibrahim, Mohd Zulfadzli Shaban, Munirah
author_facet	Mohd Jan, Nurul Hafiza Zulkifly, Ahmad Hafi Ibrahim, Mohd Zulfadzli Shaban, Munirah
author_sort	Mohd Jan, Nurul Hafiza
title	Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
title_short	Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
title_full	Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
title_fullStr	Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
title_full_unstemmed	Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result
title_sort	early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – preliminary result
publishDate	2017
url	http://irep.iium.edu.my/57853/ http://irep.iium.edu.my/57853/ http://irep.iium.edu.my/57853/21/57853.pdf
first_indexed	2023-09-18T21:21:47Z
last_indexed	2023-09-18T21:21:47Z
_version_	1777411930162462720

Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result

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