Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice

Data on acute toxicity and safety of fenugreek still not sufficient. Hence the objective of this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) in vivo. Twelve male Swiss albino mice, were randomly divided into control (C), and three treatment (T1, T2, and T3)...

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Main Authors:	Hamad Al-Farisi, Hamad Abdulsalam, Allow, Ahmed Kaid Naji, Hamdan, Asmah Hanim, Hamad Mohamed, Zenab B.
Format:	Article
Language:	English
Published:	Elixir Publishers 2017
Subjects:	RA0421 Public health. Hygiene. Preventive Medicine
Online Access:	http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/1/ASSESSMENT%20OF%20ACUTE%20LIVER%20TOXICITY%20OF%20TRIGONELLA%20FOENUM-GRAECUM%20My%20second%20published%20paper%201495453727_ELIXIR2017025583B%20%281%29.pdf

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spelling	iium-577042018-01-12T10:23:47Z http://irep.iium.edu.my/57704/ Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice Hamad Al-Farisi, Hamad Abdulsalam Allow, Ahmed Kaid Naji Hamdan, Asmah Hanim Hamad Mohamed, Zenab B. RA0421 Public health. Hygiene. Preventive Medicine Data on acute toxicity and safety of fenugreek still not sufficient. Hence the objective of this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) in vivo. Twelve male Swiss albino mice, were randomly divided into control (C), and three treatment (T1, T2, and T3) groups (n = 3 each). T1, T2, and T3 were given 3gm, 6gm, and 9gm/kg body weight FSA respectively. Intragastric divided doses of FSA were given as per OECD guidelines 425. Continuous observation of signs of acute toxicity and survival set up. Body weight was measured every 3 days, blood glucose level was measured after 6 hours, then on days 3, 7, and 14. Liver function test was measured on days 3 and 14 which is the day of sacrificing the mice. Liver was dissected and processed for histopathological examination. Administration of 9g/kg body weight of FSA showed 66.7% survival rate, while the lower FSA doses showed 100% survival. 3, 6, and 9g/kg body weight FSA failed to induce any signs of acute toxicity. Furthermore, no significant effect shown on mice body weight, blood glucose level and liver enzymes. Histologically, all FSA administered doses showed mild portal inflammation, mild mononuclear cell infiltration in hepatic parenchyma, in addition to mild bile stasis induced only in mice received 9g/kg of FSA. Conclusion: FSA showed minimum lethal oral dose and mild liver histopathological inflammatory changes Elixir Publishers 2017-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57704/1/ASSESSMENT%20OF%20ACUTE%20LIVER%20TOXICITY%20OF%20TRIGONELLA%20FOENUM-GRAECUM%20My%20second%20published%20paper%201495453727_ELIXIR2017025583B%20%281%29.pdf Hamad Al-Farisi, Hamad Abdulsalam and Allow, Ahmed Kaid Naji and Hamdan, Asmah Hanim and Hamad Mohamed, Zenab B. (2017) Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice. Elixir International Journal : Hormones and Signaling, May 2017. pp. 46697-46703. ISSN 2229-712X http://www.elixirpublishers.com/index.php?route=articles/archives&month=May&year=2017&limit=100
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RA0421 Public health. Hygiene. Preventive Medicine
spellingShingle	RA0421 Public health. Hygiene. Preventive Medicine Hamad Al-Farisi, Hamad Abdulsalam Allow, Ahmed Kaid Naji Hamdan, Asmah Hanim Hamad Mohamed, Zenab B. Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
description	Data on acute toxicity and safety of fenugreek still not sufficient. Hence the objective of this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) in vivo. Twelve male Swiss albino mice, were randomly divided into control (C), and three treatment (T1, T2, and T3) groups (n = 3 each). T1, T2, and T3 were given 3gm, 6gm, and 9gm/kg body weight FSA respectively. Intragastric divided doses of FSA were given as per OECD guidelines 425. Continuous observation of signs of acute toxicity and survival set up. Body weight was measured every 3 days, blood glucose level was measured after 6 hours, then on days 3, 7, and 14. Liver function test was measured on days 3 and 14 which is the day of sacrificing the mice. Liver was dissected and processed for histopathological examination. Administration of 9g/kg body weight of FSA showed 66.7% survival rate, while the lower FSA doses showed 100% survival. 3, 6, and 9g/kg body weight FSA failed to induce any signs of acute toxicity. Furthermore, no significant effect shown on mice body weight, blood glucose level and liver enzymes. Histologically, all FSA administered doses showed mild portal inflammation, mild mononuclear cell infiltration in hepatic parenchyma, in addition to mild bile stasis induced only in mice received 9g/kg of FSA. Conclusion: FSA showed minimum lethal oral dose and mild liver histopathological inflammatory changes
format	Article
author	Hamad Al-Farisi, Hamad Abdulsalam Allow, Ahmed Kaid Naji Hamdan, Asmah Hanim Hamad Mohamed, Zenab B.
author_facet	Hamad Al-Farisi, Hamad Abdulsalam Allow, Ahmed Kaid Naji Hamdan, Asmah Hanim Hamad Mohamed, Zenab B.
author_sort	Hamad Al-Farisi, Hamad Abdulsalam
title	Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
title_short	Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
title_full	Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
title_fullStr	Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
title_full_unstemmed	Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
title_sort	assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
publisher	Elixir Publishers
publishDate	2017
url	http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/1/ASSESSMENT%20OF%20ACUTE%20LIVER%20TOXICITY%20OF%20TRIGONELLA%20FOENUM-GRAECUM%20My%20second%20published%20paper%201495453727_ELIXIR2017025583B%20%281%29.pdf
first_indexed	2023-09-18T21:21:35Z
last_indexed	2023-09-18T21:21:35Z
_version_	1777411917917192192

Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice

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