Assessment of acute liver toxicity of trigonella foenum-graecum (fenugreek) seeds aqueous extract in male mice
Data on acute toxicity and safety of fenugreek still not sufficient. Hence the objective of this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) in vivo. Twelve male Swiss albino mice, were randomly divided into control (C), and three treatment (T1, T2, and T3)...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elixir Publishers
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/ http://irep.iium.edu.my/57704/1/ASSESSMENT%20OF%20ACUTE%20LIVER%20TOXICITY%20OF%20TRIGONELLA%20FOENUM-GRAECUM%20My%20second%20published%20paper%201495453727_ELIXIR2017025583B%20%281%29.pdf |
| Summary: | Data on acute toxicity and safety of fenugreek still not sufficient. Hence the objective of
this study is to investigate the acute toxicity of fenugreek seeds aqueous extract (FSA) in
vivo. Twelve male Swiss albino mice, were randomly divided into control (C), and three
treatment (T1, T2, and T3) groups (n = 3 each). T1, T2, and T3 were given 3gm, 6gm, and
9gm/kg body weight FSA respectively. Intragastric divided doses of FSA were given as
per OECD guidelines 425. Continuous observation of signs of acute toxicity and survival
set up. Body weight was measured every 3 days, blood glucose level was measured after
6 hours, then on days 3, 7, and 14. Liver function test was measured on days 3 and 14
which is the day of sacrificing the mice. Liver was dissected and processed for
histopathological examination. Administration of 9g/kg body weight of FSA showed
66.7% survival rate, while the lower FSA doses showed 100% survival. 3, 6, and 9g/kg
body weight FSA failed to induce any signs of acute toxicity. Furthermore, no significant
effect shown on mice body weight, blood glucose level and liver enzymes.
Histologically, all FSA administered doses showed mild portal inflammation, mild
mononuclear cell infiltration in hepatic parenchyma, in addition to mild bile stasis
induced only in mice received 9g/kg of FSA. Conclusion: FSA showed minimum lethal
oral dose and mild liver histopathological inflammatory changes |
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