Modification and characterization of phytase for animal feed production

Phytases catalyze the hydrolysis of inorganic phosphate from phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contribute to elimination of beneficial nutrient for...

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Main Authors:	Noorbatcha, Ibrahim Ali, Samsudin, Nurhusna, Mohd. Salleh, Hamzah
Format:	Article
Language:	English
Published:	Asian Network for Scientific Information 2009
Subjects:	SB Plant culture
Online Access:	http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/1/Modification_and_characterization_of_phytase_for_animal_feed_production.pdf

id	iium-577
recordtype	eprints
spelling	iium-5772019-11-07T07:04:39Z http://irep.iium.edu.my/577/ Modification and characterization of phytase for animal feed production Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Mohd. Salleh, Hamzah SB Plant culture Phytases catalyze the hydrolysis of inorganic phosphate from phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contribute to elimination of beneficial nutrient for growth therefore contribute to land pollution, eutrophication of ground water and aquatic environment. Besides, it leads to the negative effect on vitamin utilization that lead to the emaciation, retarded growth and reproductive failure to animal. Due to the importance of, microbial sources for the commercial production of phytases, we have selected waste water bacterium phytase as the subject of interest in this study. In silico experiment is used to identify and examine the active site of waste water bacterium phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength. Multiple mutations of M216R/E219R/H17A, M216R/E219R/F254E and some other multiple mutations showed improvement in the binding strength, primarily due to the addition of hydrogen bond with the adjacent residues. Automated docking based on genetic algorithm is used to dock the phytate in the active site and Partial Mean Force (PMF) scoring is used to calculate the strength of the binding before and after mutation. Asian Network for Scientific Information 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/577/1/Modification_and_characterization_of_phytase_for_animal_feed_production.pdf Noorbatcha, Ibrahim Ali and Samsudin, Nurhusna and Mohd. Salleh, Hamzah (2009) Modification and characterization of phytase for animal feed production. Journal of Applied Sciences, 9 (17). pp. 3080-3085. ISSN 1812-5654 E-ISSN 1812-5662 http://scialert.net/qredirect.php?doi=jas.2009.3080.3085&linkid=pdf DOI: 10.3923/jas.2009.3080.3085
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Mohd. Salleh, Hamzah Modification and characterization of phytase for animal feed production
description	Phytases catalyze the hydrolysis of inorganic phosphate from phytic acid and are able to improve the nutritional quality of phytate rich diet. Monogastric animal such as poultry and fish have lack of significant activity to hydrolyze phytate that contribute to elimination of beneficial nutrient for growth therefore contribute to land pollution, eutrophication of ground water and aquatic environment. Besides, it leads to the negative effect on vitamin utilization that lead to the emaciation, retarded growth and reproductive failure to animal. Due to the importance of, microbial sources for the commercial production of phytases, we have selected waste water bacterium phytase as the subject of interest in this study. In silico experiment is used to identify and examine the active site of waste water bacterium phytase. The factors influencing the ligand binding strength in the active site is analyzed and computational site directed mutagenesis experiments were carried out to evaluate the effects of mutations on the binding strength. Multiple mutations of M216R/E219R/H17A, M216R/E219R/F254E and some other multiple mutations showed improvement in the binding strength, primarily due to the addition of hydrogen bond with the adjacent residues. Automated docking based on genetic algorithm is used to dock the phytate in the active site and Partial Mean Force (PMF) scoring is used to calculate the strength of the binding before and after mutation.
format	Article
author	Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Mohd. Salleh, Hamzah
author_facet	Noorbatcha, Ibrahim Ali Samsudin, Nurhusna Mohd. Salleh, Hamzah
author_sort	Noorbatcha, Ibrahim Ali
title	Modification and characterization of phytase for animal feed production
title_short	Modification and characterization of phytase for animal feed production
title_full	Modification and characterization of phytase for animal feed production
title_fullStr	Modification and characterization of phytase for animal feed production
title_full_unstemmed	Modification and characterization of phytase for animal feed production
title_sort	modification and characterization of phytase for animal feed production
publisher	Asian Network for Scientific Information
publishDate	2009
url	http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/ http://irep.iium.edu.my/577/1/Modification_and_characterization_of_phytase_for_animal_feed_production.pdf
first_indexed	2023-09-18T20:07:43Z
last_indexed	2023-09-18T20:07:43Z
_version_	1777407270408159232

Modification and characterization of phytase for animal feed production

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