Optimization of plant growth regulators for Citrus suhuiensis callus induction

Correct types and concentration of plant growth regulators (PGRs) will enhance and optimize the growth of callus cultures. This paper reported the effects of several types of cytokinins (2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA)) and auxins (6–Benzylaminopurine (BAP)...

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Main Authors:	M. Fathil, Nur Alia, Mohamad Puad, Noor Illi, Amid, Azura, Azmi, Azlin Suhaida, Ibrahim, Rusli
Format:	Article
Language:	English
Published:	Malaysian Society for Molecular Biology and Biotechnology 2017
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://irep.iium.edu.my/57698/ http://irep.iium.edu.my/57698/ http://irep.iium.edu.my/57698/13/57698_Asia%20Pacific%20_complete.pdf

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spelling	iium-576982018-01-31T17:31:57Z http://irep.iium.edu.my/57698/ Optimization of plant growth regulators for Citrus suhuiensis callus induction M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli Q Science (General) T Technology (General) Correct types and concentration of plant growth regulators (PGRs) will enhance and optimize the growth of callus cultures. This paper reported the effects of several types of cytokinins (2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA)) and auxins (6–Benzylaminopurine (BAP) and kinetin) on the callus induction of C. suhuiensis. The cotyledons from C. suhuiensis seeds were excised as the explant and cultured on Murashige and Skoog (MS) media, 3% (w/v) sugar, 0.05% (w/v) malt extract and 0.25% (w/v) agar under the continuous dark condition supplemented with the chosen PGRs at the concentration range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L. The growth of callus at each treatment was measured as gram (g) of fresh weight and percentage of callus induction. The results showed that 1.0 mg/L 2,4-D gave the highest growth of callus (0.15 g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from the Design Expert® software version 9.0 was used to obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus cultures. It was observed that the highest amount of callus culture induced was 0.218 g when the media was supplemented with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin. Malaysian Society for Molecular Biology and Biotechnology 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57698/13/57698_Asia%20Pacific%20_complete.pdf M. Fathil, Nur Alia and Mohamad Puad, Noor Illi and Amid, Azura and Azmi, Azlin Suhaida and Ibrahim, Rusli (2017) Optimization of plant growth regulators for Citrus suhuiensis callus induction. Asia-Pacific Journal of Molecular Biology and Biotechnology, 25 (1). pp. 1-7. ISSN 0128-7451 http://www.msmbb.org.my/apjmbb/html251/251cont.htm
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli Optimization of plant growth regulators for Citrus suhuiensis callus induction
description	Correct types and concentration of plant growth regulators (PGRs) will enhance and optimize the growth of callus cultures. This paper reported the effects of several types of cytokinins (2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA)) and auxins (6–Benzylaminopurine (BAP) and kinetin) on the callus induction of C. suhuiensis. The cotyledons from C. suhuiensis seeds were excised as the explant and cultured on Murashige and Skoog (MS) media, 3% (w/v) sugar, 0.05% (w/v) malt extract and 0.25% (w/v) agar under the continuous dark condition supplemented with the chosen PGRs at the concentration range of 0.5, 1.0, 2.0, 3.0 and 4.0 mg/L. The growth of callus at each treatment was measured as gram (g) of fresh weight and percentage of callus induction. The results showed that 1.0 mg/L 2,4-D gave the highest growth of callus (0.15 g and 100% callus percentage). After identifying the effective PGRs, Central Composite Design (CCD) from the Design Expert® software version 9.0 was used to obtain the optimum concentration of cytokinin and auxin on C. suhuiensis callus cultures. It was observed that the highest amount of callus culture induced was 0.218 g when the media was supplemented with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin.
format	Article
author	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli
author_facet	M. Fathil, Nur Alia Mohamad Puad, Noor Illi Amid, Azura Azmi, Azlin Suhaida Ibrahim, Rusli
author_sort	M. Fathil, Nur Alia
title	Optimization of plant growth regulators for Citrus suhuiensis callus induction
title_short	Optimization of plant growth regulators for Citrus suhuiensis callus induction
title_full	Optimization of plant growth regulators for Citrus suhuiensis callus induction
title_fullStr	Optimization of plant growth regulators for Citrus suhuiensis callus induction
title_full_unstemmed	Optimization of plant growth regulators for Citrus suhuiensis callus induction
title_sort	optimization of plant growth regulators for citrus suhuiensis callus induction
publisher	Malaysian Society for Molecular Biology and Biotechnology
publishDate	2017
url	http://irep.iium.edu.my/57698/ http://irep.iium.edu.my/57698/ http://irep.iium.edu.my/57698/13/57698_Asia%20Pacific%20_complete.pdf
first_indexed	2023-09-18T21:21:35Z
last_indexed	2023-09-18T21:21:35Z
_version_	1777411917626736640

Optimization of plant growth regulators for Citrus suhuiensis callus induction

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