The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan

Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba m...

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Main Authors:	Aznan, Anis Amira, Mat Yusof, Afzan, Md Isa, Muhammad Lokman, Mat Amin, Nakisah
Format:	Conference or Workshop Item
Language:	English
Published:	2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/57566/ http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf

id	iium-57566
recordtype	eprints
spelling	iium-575662017-07-13T02:00:10Z http://irep.iium.edu.my/57566/ The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan Aznan, Anis Amira Mat Yusof, Afzan Md Isa, Muhammad Lokman Mat Amin, Nakisah Q Science (General) Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba may be deposited in air ventilation systems with maintaining practices. This could predispose discomfort and diseases to human health especially towards immunosuppressed individuals. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. By using a sterile swab, the dust samples were collected from filters of the air ventilation systems. The dust samples were cultured on the non-nutrient agar plates with inactivate Escherichia coli and incubated in 37ºC incubator. The plates were examined daily for any presence of Acanthamoeba cysts up to 14 days of incubation. The results showed that all 75 dust samples collected were negative. The microscopic examination showed that all non-nutrient agar (NNA) plates were negative for Acantamoeba from day 1 until day 14 of the incubation period. However, fungal growth was observed on the non-nutrient agar (NNA) plates after day 4 of incubation. The negative findings of this study may have been influenced by the environmental factor in Kuantan. Besides that, there are also limitations in the method employed in this study where direct swabs from air ventilation systems was were used instead of using a more specific method such as establishing proper sampling points with air quality instruments to collect air samples. Thus, further studies should consider in order to address these limitations and conditions. 2017-05-12 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf Aznan, Anis Amira and Mat Yusof, Afzan and Md Isa, Muhammad Lokman and Mat Amin, Nakisah (2017) The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan. In: 8th Malaysian Symposium of Biomedical Science, 12th-14th May 2017, Kuantan, Pahang Darul Makmur. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Aznan, Anis Amira Mat Yusof, Afzan Md Isa, Muhammad Lokman Mat Amin, Nakisah The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
description	Acanthamoeba, a free-living amoeba (FLA) which is abundant in a wide range of the environmental conditions including water, soil and air. Since Acanthamoeba is opportunistic pathogen, some of its species are found to be responsible for serious human diseases. Cysts and trophozoites of Acanthamoeba may be deposited in air ventilation systems with maintaining practices. This could predispose discomfort and diseases to human health especially towards immunosuppressed individuals. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. Acanthamoeba was isolated from dust samples taken from air ventilation systems in selected buildings in Kuantan. By using a sterile swab, the dust samples were collected from filters of the air ventilation systems. The dust samples were cultured on the non-nutrient agar plates with inactivate Escherichia coli and incubated in 37ºC incubator. The plates were examined daily for any presence of Acanthamoeba cysts up to 14 days of incubation. The results showed that all 75 dust samples collected were negative. The microscopic examination showed that all non-nutrient agar (NNA) plates were negative for Acantamoeba from day 1 until day 14 of the incubation period. However, fungal growth was observed on the non-nutrient agar (NNA) plates after day 4 of incubation. The negative findings of this study may have been influenced by the environmental factor in Kuantan. Besides that, there are also limitations in the method employed in this study where direct swabs from air ventilation systems was were used instead of using a more specific method such as establishing proper sampling points with air quality instruments to collect air samples. Thus, further studies should consider in order to address these limitations and conditions.
format	Conference or Workshop Item
author	Aznan, Anis Amira Mat Yusof, Afzan Md Isa, Muhammad Lokman Mat Amin, Nakisah
author_facet	Aznan, Anis Amira Mat Yusof, Afzan Md Isa, Muhammad Lokman Mat Amin, Nakisah
author_sort	Aznan, Anis Amira
title	The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_short	The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_full	The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_fullStr	The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_full_unstemmed	The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan
title_sort	isolation and identification of acanthamoeba from air ventilation system samples in selected buildings in kuantan
publishDate	2017
url	http://irep.iium.edu.my/57566/ http://irep.iium.edu.my/57566/1/Conference%20Malaysian%20Symposium%202017%20a.pdf
first_indexed	2023-09-18T21:21:23Z
last_indexed	2023-09-18T21:21:23Z
_version_	1777411904521633792

The isolation and identification of Acanthamoeba from air ventilation system samples in selected buildings in Kuantan

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