Screening of effective markers for Mesophilic bacterium growth using factorial experimental design

Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mesophilic bacterium, Bacillus cereu...

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Main Authors:	Ahmad, Mani Malam, Mohd Azoddien, Abd. Aziz, Mohd Zahari, Mior Ahmad Khusairi, Abu Seman, Mazrul Nizam, Jami, Mohammed Saedi
Format:	Article
Language:	English
Published:	Science & Engineering Research Support soCiety 2017
Subjects:	TP155 Chemical engineering
Online Access:	http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf

id	iium-57550
recordtype	eprints
spelling	iium-575502018-01-31T17:35:52Z http://irep.iium.edu.my/57550/ Screening of effective markers for Mesophilic bacterium growth using factorial experimental design Ahmad, Mani Malam Mohd Azoddien, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi TP155 Chemical engineering Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mesophilic bacterium, Bacillus cereus (ATCC 14579) in a batch type medium of orbital shake flasks. Screening of linear and interactions effects of parametric markers was evaluated by two level (24) factorial design of experiment (DOE). Growth was found to respond significantly to nutrient concentration coupled with other independent variables. The factorial models established from experimental design to investigate the individual and interactions effects toward the response within the selected variables of nutrient concentration (4-16 g l-1), temperature (30 oC – 42 oC), agitation (140 rpm-200 rpm) and acclimatization time (24 hours-72 hours). These were statistically validated using analysis of variance (ANOVA). The results revealed that the model terms were all significant with F-value of 251.07 at (p =0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the influence is in the ascending order A > B > AB > C > AC. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the models is maintained. It could, therefore, be concluded that nutrient concentration, temperature and acclimatization time were variables that greatly limit growth at a specific range. Science & Engineering Research Support soCiety 2017-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57550/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf Ahmad, Mani Malam and Mohd Azoddien, Abd. Aziz and Mohd Zahari, Mior Ahmad Khusairi and Abu Seman, Mazrul Nizam and Jami, Mohammed Saedi (2017) Screening of effective markers for Mesophilic bacterium growth using factorial experimental design. International Journal of Bio-Science and Bio-Technology, 9 (3). pp. 59-74. ISSN 2233-7849 http://www.sersc.org/journals/IJBSBT/vol9_no3.php 10.14257/ibsbt.2017.9.3.06
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TP155 Chemical engineering
spellingShingle	TP155 Chemical engineering Ahmad, Mani Malam Mohd Azoddien, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
description	Attempts to gain an insight and present bacterial growth pattern from OVAT perspectives are quite defective and strenuous. The present study aimed at evaluating this concept from the different perspective by evaluating factors with more impacts on the growth of a mesophilic bacterium, Bacillus cereus (ATCC 14579) in a batch type medium of orbital shake flasks. Screening of linear and interactions effects of parametric markers was evaluated by two level (24) factorial design of experiment (DOE). Growth was found to respond significantly to nutrient concentration coupled with other independent variables. The factorial models established from experimental design to investigate the individual and interactions effects toward the response within the selected variables of nutrient concentration (4-16 g l-1), temperature (30 oC – 42 oC), agitation (140 rpm-200 rpm) and acclimatization time (24 hours-72 hours). These were statistically validated using analysis of variance (ANOVA). The results revealed that the model terms were all significant with F-value of 251.07 at (p =0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the influence is in the ascending order A > B > AB > C > AC. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the models is maintained. It could, therefore, be concluded that nutrient concentration, temperature and acclimatization time were variables that greatly limit growth at a specific range.
format	Article
author	Ahmad, Mani Malam Mohd Azoddien, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi
author_facet	Ahmad, Mani Malam Mohd Azoddien, Abd. Aziz Mohd Zahari, Mior Ahmad Khusairi Abu Seman, Mazrul Nizam Jami, Mohammed Saedi
author_sort	Ahmad, Mani Malam
title	Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
title_short	Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
title_full	Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
title_fullStr	Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
title_full_unstemmed	Screening of effective markers for Mesophilic bacterium growth using factorial experimental design
title_sort	screening of effective markers for mesophilic bacterium growth using factorial experimental design
publisher	Science & Engineering Research Support soCiety
publishDate	2017
url	http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/ http://irep.iium.edu.my/57550/1/Screening%20of%20Effective%20Markers%20for%20Mesophilic%20Bacterium%20Growth.pdf
first_indexed	2023-09-18T21:21:21Z
last_indexed	2023-09-18T21:21:21Z
_version_	1777411903222448128

Screening of effective markers for Mesophilic bacterium growth using factorial experimental design

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