Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan

Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S...

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Main Authors:	Mat Yusof, Afzan, Md Isa, Muhammad Lokman
Format:	Article
Language:	English English
Published:	Malaysian Society of Applied Biology 2017
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/57533/ http://irep.iium.edu.my/57533/ http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf

id	iium-57533
recordtype	eprints
spelling	iium-575332017-09-29T03:52:00Z http://irep.iium.edu.my/57533/ Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan Mat Yusof, Afzan Md Isa, Muhammad Lokman Q Science (General) Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S rRNA gene. Besides, this study aims to investigate the association between Cryptosporidium infection and the age of dairy cattle. 375 stool specimens of dairy cattle were collected and concentrated with formal-ether concentration technique. The Cryptosporidium oocysts were detected with modified Ziehl Neelsen staining. Cryptosporidium species was identified by nested PCR amplification of 18S rRNA gene. Based on microscopic examination, 16.3% (61/375) dairy cattle were positive for Cryptosporidium infection. This research has shown that the highest prevalence of Cryptosporidium was recorded in calves with the percentage of 17.4% (12/69), followed by adult cattle and yearling with the percentage of 16.1% (29/180) and 15.9% (20/126), respectively. The findings demonstrated that there was no significant difference (p > 0.05) in Cryptosporidium infection rates by age. Molecular characterization revealed that the species of Cryptosporidium found in dairy cattle was C. ryanae. The present study suggested that proper hygiene practices must be practiced by farmers in order to control the Cryptosporidium infection. Malaysian Society of Applied Biology 2017-06 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf application/pdf en http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf Mat Yusof, Afzan and Md Isa, Muhammad Lokman (2017) Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan. Malaysian Applied Biology, 46 (2). pp. 21-27. ISSN 0126-8643 http://mabjournal.com/images/46_2_June_2017/46_02_03.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Mat Yusof, Afzan Md Isa, Muhammad Lokman Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
description	Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S rRNA gene. Besides, this study aims to investigate the association between Cryptosporidium infection and the age of dairy cattle. 375 stool specimens of dairy cattle were collected and concentrated with formal-ether concentration technique. The Cryptosporidium oocysts were detected with modified Ziehl Neelsen staining. Cryptosporidium species was identified by nested PCR amplification of 18S rRNA gene. Based on microscopic examination, 16.3% (61/375) dairy cattle were positive for Cryptosporidium infection. This research has shown that the highest prevalence of Cryptosporidium was recorded in calves with the percentage of 17.4% (12/69), followed by adult cattle and yearling with the percentage of 16.1% (29/180) and 15.9% (20/126), respectively. The findings demonstrated that there was no significant difference (p > 0.05) in Cryptosporidium infection rates by age. Molecular characterization revealed that the species of Cryptosporidium found in dairy cattle was C. ryanae. The present study suggested that proper hygiene practices must be practiced by farmers in order to control the Cryptosporidium infection.
format	Article
author	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_facet	Mat Yusof, Afzan Md Isa, Muhammad Lokman
author_sort	Mat Yusof, Afzan
title	Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_short	Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_full	Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_fullStr	Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_full_unstemmed	Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_sort	prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in kuantan
publisher	Malaysian Society of Applied Biology
publishDate	2017
url	http://irep.iium.edu.my/57533/ http://irep.iium.edu.my/57533/ http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf
first_indexed	2023-09-18T21:21:20Z
last_indexed	2023-09-18T21:21:20Z
_version_	1777411901935845376

Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan

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