Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum

Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin,...

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Main Authors:	Aminudin, Nurul Iman, Ahmad, Farediah, Bakhtiar, M. Taher, Mohamed Zulkifli, Razauden
Format:	Conference or Workshop Item
Language:	English English
Published:	2018
Subjects:	RS192 Materia Medica-Pharmaceutical Technology
Online Access:	http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf http://irep.iium.edu.my/57430/7/69556.pdf

id	iium-57430
recordtype	eprints
spelling	iium-574302019-01-30T04:24:23Z http://irep.iium.edu.my/57430/ Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden RS192 Materia Medica-Pharmaceutical Technology Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin, chromanone carboxylic acid and flavonoid which showed anti-HIV, cytotoxicity and antimicrobial properties. In this study, phytochemicals studies on the barks and leaves of Calophyllum incrassatum M.R. Henderson & Wyatt-Smith were carried out. Cold extraction method was employed by using dichloromethane, ethyl acetate and methanol to give the respective crude extracts. Four new 4-substituted coumarins, namely, incrassamarin A, B, C and D were successfully isolated from the dichloromethane extracts of the barks and leaves by size-exclusion recycling HPLC and column chromatography techniques. The structures of the compounds were elucidated by spectral analysis including NMR, IR, MS and X-ray crystallography. The compounds were tested for their cytotoxic activity towards human breast adenocarcinoma (MCF-7) and human non-small cell lung carcinoma (A-549) cell lines by MTT assay and α-glucosidase enzymatic inhibitory activity. Incrassamarin A displayed cytotoxic activity against A-549 cell lines with IC50 87.71 mg/ml and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 mM. 2018 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf application/pdf en http://irep.iium.edu.my/57430/7/69556.pdf Aminudin, Nurul Iman and Ahmad, Farediah and Bakhtiar, M. Taher and Mohamed Zulkifli, Razauden (2018) Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum. In: 34th International Conference on Natural Products 2018 (ICNP 2018), 19th-21st March 2018, Penang, Malaysia. (Unpublished) http://www.icnp2018.usm.my/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RS192 Materia Medica-Pharmaceutical Technology
spellingShingle	RS192 Materia Medica-Pharmaceutical Technology Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
description	Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin, chromanone carboxylic acid and flavonoid which showed anti-HIV, cytotoxicity and antimicrobial properties. In this study, phytochemicals studies on the barks and leaves of Calophyllum incrassatum M.R. Henderson & Wyatt-Smith were carried out. Cold extraction method was employed by using dichloromethane, ethyl acetate and methanol to give the respective crude extracts. Four new 4-substituted coumarins, namely, incrassamarin A, B, C and D were successfully isolated from the dichloromethane extracts of the barks and leaves by size-exclusion recycling HPLC and column chromatography techniques. The structures of the compounds were elucidated by spectral analysis including NMR, IR, MS and X-ray crystallography. The compounds were tested for their cytotoxic activity towards human breast adenocarcinoma (MCF-7) and human non-small cell lung carcinoma (A-549) cell lines by MTT assay and α-glucosidase enzymatic inhibitory activity. Incrassamarin A displayed cytotoxic activity against A-549 cell lines with IC50 87.71 mg/ml and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 mM.
format	Conference or Workshop Item
author	Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden
author_facet	Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden
author_sort	Aminudin, Nurul Iman
title	Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
title_short	Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
title_full	Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
title_fullStr	Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
title_full_unstemmed	Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
title_sort	isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
publishDate	2018
url	http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf http://irep.iium.edu.my/57430/7/69556.pdf
first_indexed	2023-09-18T21:21:10Z
last_indexed	2023-09-18T21:21:10Z
_version_	1777411891692306432

Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum

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