Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy

Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimen...

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Main Authors:	Ahmad Azam, Amalina, Pariyani, Raghunath, Ismail, Intan Safinar, Ismail, Amin, Khatib, Alfi, Abas, Faridah, Khozirah, Shaar
Format:	Article
Language:	English English
Published:	BioMed Central 2017
Subjects:	RC Internal medicine RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf

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spelling	iium-574132018-03-09T07:56:23Z http://irep.iium.edu.my/57413/ Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar RC Internal medicine RS Pharmacy and materia medica Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods: Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results: OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions: The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved. BioMed Central 2017-05-25 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf application/pdf en http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf Ahmad Azam, Amalina and Pariyani, Raghunath and Ismail, Intan Safinar and Ismail, Amin and Khatib, Alfi and Abas, Faridah and Khozirah, Shaar (2017) Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy. BMC Complementary and Alternative Medicine, 17 (1). pp. 1-13. ISSN 1472-6882 https://bmccomplementalternmed.biomedcentral.com/track/pdf/10.1186/s12906-017-1777-1?site=bmccomplementalternmed.biomedcentral.com 10.1186/s12906-017-1777-1
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	RC Internal medicine RS Pharmacy and materia medica
spellingShingle	RC Internal medicine RS Pharmacy and materia medica Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
description	Background: Orthosiphon stamineus (OS) is a herb known in ethnomedicine for treating diabetes mellitus (DM). In this study, a H NMR based urine metabolomics tool has been used for the first time to identify the metabolic protective mechanism of OS in DM using Streptozotocin (STZ) induced experimental model in rats. Methods: Four different solvent extracts of OS, namely aqueous, ethanolic, 50% aqueous ethanolic and methanolic, at a dose of 500 mg/kg body weight (bw) were orally administered for 14 days to diabetic rats induced via intraperitoneal injection of 60 mg/kg bw STZ. NMR metabolomics approach using pattern recognition combined with multivariate statistical analysis was applied in the rat urine to study the resulted metabolic perturbations. Results: OS aqueous extract (OSAE) caused a reversal of DM comparable to that of 10 mg/kg bw glibenclamide. A total of 15 urinary metabolites, which levels changed significantly upon treatment were identified as the biomarkers of OSAE in diabetes. A systematic metabolic pathways analysis identified that OSAE contributed to the antidiabetic activity mainly through regulating the tricarboxylic acid cycle, glycolysis/gluconeogenesis, lipid and amino acid metabolism. Conclusions: The results of this study validated the ethnopharmacological use of OS in diabetes and unveiled the biochemical and metabolic mechanisms involved.
format	Article
author	Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar
author_facet	Ahmad Azam, Amalina Pariyani, Raghunath Ismail, Intan Safinar Ismail, Amin Khatib, Alfi Abas, Faridah Khozirah, Shaar
author_sort	Ahmad Azam, Amalina
title	Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
title_short	Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
title_full	Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
title_fullStr	Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
title_full_unstemmed	Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy
title_sort	urinary metabolomics study on the protective role of orthosiphon stamineus in streptozotocin induced diabetes mellitus in rats via 1h nmr spectroscopy
publisher	BioMed Central
publishDate	2017
url	http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/ http://irep.iium.edu.my/57413/1/57413_Urinary%20metabolomics%20_article.pdf http://irep.iium.edu.my/57413/2/57413_Urinary%20metabolomics%20_SCOPUS.pdf
first_indexed	2023-09-18T21:21:09Z
last_indexed	2023-09-18T21:21:09Z
_version_	1777411889906581504

Urinary metabolomics study on the protective role of Orthosiphon stamineus in Streptozotocin induced diabetes mellitus in rats via 1H NMR spectroscopy

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